Christina Eleanor Barkauskas, MD

Assistant Professor of Medicine
Affiliate of the Duke Regeneration Center
Campus mail Box 2629 Med Ctr, Durham, NC 27710
Phone (919) 681-6845
Email address christina.barkauskas@duke.edu

I am a physician-scientist and stem cell biologist focused on understanding the fundamental mechanisms involved in lung injury and repair. I am particularly interested in determining the critical components of the alveolar epithelial stem cell niche and the cellular crosstalk signals that dictate cell identity and behavior during steady state maintenance and repair after injury and infection. I believe this work is fundamental to better understanding the biology of the human lung and the pathogenesis of idiopathic pulmonary fibrosis (IPF), a disease that is characterized by relentless scar accumulation in the lung with only minimally effective therapy short of lung transplant.

Through my work, I have developed several areas of expertise which include the following: 1) Development and use of mouse models to genetically manipulate and lineage trace specific cell populations; 2) Isolation and characterization of murine lung cell subpopulations by flow cytometry; 3) Development and use of an ex vivo 3-dimensional organoid assay to study the self-renewal and differentiation of murine alveolar epithelial stem cells and their niche components; 4) Isolation and 3-dimensional culture of alveolar epithelial cells from fresh human lung; 5) Development and analysis of models of experimental lung fibrosis.

Education and Training

  • Advanced Training, Pulmonary & Critical Care Medicine, Duke University School of Medicine, 2011 - 2012
  • Fellowship, Pulmonary& Critical Care Medicine, Duke University School of Medicine, 2009 - 2011
  • Chief Medical Resident, Department Of Medicine, Duke University School of Medicine, 2008 - 2009
  • Fellow in Pulmonary Medicine, Medicine, Duke University, 2007 - 2008
  • Resident, Medicine, Duke University, 2004 - 2007
  • M.D., Duke University School of Medicine, 2004

Publications

Jain, Rajan, Christina E. Barkauskas, Norifumi Takeda, Emily J. Bowie, Haig Aghajanian, Qiaohong Wang, Arun Padmanabhan, et al. “Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.” Nat Commun 6 (April 13, 2015): 6727. https://doi.org/10.1038/ncomms7727.

PMID
25865356
Full Text

Hogan, Brigid L. M., Christina E. Barkauskas, Harold A. Chapman, Jonathan A. Epstein, Rajan Jain, Connie C. W. Hsia, Laura Niklason, et al. “Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function.” Cell Stem Cell 15, no. 2 (August 7, 2014): 123–38. https://doi.org/10.1016/j.stem.2014.07.012.

PMID
25105578
Full Text

Barkauskas, Christina E., and Paul W. Noble. “Cellular mechanisms of tissue fibrosis. 7. New insights into the cellular mechanisms of pulmonary fibrosis.” Am J Physiol Cell Physiol 306, no. 11 (June 1, 2014): C987–96. https://doi.org/10.1152/ajpcell.00321.2013.

PMID
24740535
Full Text

Barkauskas, Christina E., Michael J. Cronce, Craig R. Rackley, Emily J. Bowie, Douglas R. Keene, Barry R. Stripp, Scott H. Randell, Paul W. Noble, and Brigid L. M. Hogan. “Type 2 alveolar cells are stem cells in adult lung.” J Clin Invest 123, no. 7 (July 2013): 3025–36. https://doi.org/10.1172/JCI68782.

PMID
23921127
Full Text

Wansleeben, Carolien, Christina E. Barkauskas, Jason R. Rock, and Brigid L. M. Hogan. “Stem cells of the adult lung: their development and role in homeostasis, regeneration, and disease.” Wiley Interdiscip Rev Dev Biol 2, no. 1 (January 2013): 131–48. https://doi.org/10.1002/wdev.58.

PMID
23799633
Full Text

Noble, Paul W., Christina E. Barkauskas, and Dianhua Jiang. “Pulmonary fibrosis: patterns and perpetrators.” J Clin Invest 122, no. 8 (August 2012): 2756–62. https://doi.org/10.1172/JCI60323.

PMID
22850886
Full Text

Rock, Jason R., Christina E. Barkauskas, Michael J. Cronce, Yan Xue, Jeffrey R. Harris, Jiurong Liang, Paul W. Noble, and Brigid L. M. Hogan. “Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition.” Proc Natl Acad Sci U S A 108, no. 52 (December 27, 2011): E1475–83. https://doi.org/10.1073/pnas.1117988108.

PMID
22123957
Full Text

Barkauskas, C. E., J. R. Rock, M. Cronce, C. Liang, P. W. Noble, and B. L. M. Hogan. “Lineage Tracing Of Mature Type 2 Alveolar Epithelial Cells Reveals New Insights Into Alveolar Maintenance And Repair.” American Journal of Respiratory and Critical Care Medicine 183 (January 1, 2011).

Scholars@Duke

Crowley, Matthew J., Christina E. Barkauskas, F Douglas Srygley, Evan P. Kransdorf, Thomas W. LeBlanc, David L. Simel, and Diana B. McNeill. “A comparative resident site visit project: a novel approach for implementing programmatic change in the duty hours era.” Acad Med 85, no. 7 (July 2010): 1140–46. https://doi.org/10.1097/ACM.0b013e3181e18cee.

PMID
20592509
Full Text

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