Gerard Conrad Blobe, MD, PhD

Professor of Medicine
Professor of Pharmacology and Cancer Biology
Associate of the Duke Initiative for Science & Society
Member of the Duke Cancer Institute
Campus mail B354 Levine Science Research Center, 450 Research Drive, Durham, NC 27708
Phone (919) 668-6688

Our laboratory focuses on transforming growth factor-ß (TGF-ß) superfamily signal transduction pathways, and specifically, the role of these pathways in cancer biology. The TGF-ß superfamily is comprised of a number of polypeptide growth factors, including TGF-βs, bone morphogenetic proteins (BMPs) and activin) that regulate growth, differentiation and morphogenesis in a cell and context specific manner. TGF-ß and the TGF-ß signaling pathway have a dichotomous role in cancer biology, as both tumor-suppressor genes (presumably as regulators of cellular proliferation, differentiation and apoptosis) and as tumor promoters (presumably as regulators of cellular motility, adhesion, angiogenesis and the immune system). This dichotomy of TGF-ß function remains a fundamental problem in the field both in terms of understanding the mechanism of action of the TGF-ß pathway, and directly impacting our ability to target this pathway for the chemoprevention or treatment of human cancers. Resistance to the tumor suppressor effects of TGF-ß is also a common feature of epithelial-derived human cancers (breast, colon, lung, pancreatic, prostate), however, mechanisms for TGF-ß resistance remain undefined in the majority of cases. TGF-ß regulates cellular processes by binding to three high affinity cell surface receptors, the type I, type II, and type III receptors. Recent studies by our laboratory and others have established the type III TGF-ß receptor as a critical mediator/regulator of TGF-ß signaling. Specifically we have demonstrated that regulating type III TGF-ß receptor expression levels is sufficient to regulate TGF-ß signaling, and that decreased type III receptor expression is a common phenomenon in human cancers, resulting in cancer progression. The role of the type III TGF-ß receptor and type III TGF-ß receptor-interacting proteins in TGF-ß signaling and cancer biology and the epithelial to mesenchymal transition that occurs in human breast, colon and pancreatic cancers are currently being investigated using a multidisciplinary approach.
TGF-ß and the TGF-ß superfamily signaling pathways also have an important role in vascular biology. Indeed, mutations in two endothelial specific TGF-ß superfamily receptors, endoglin and ALK-1 (a type I receptor in the TGF-ß family), are responsible for the human vascular disease, hereditary hemorrhagic telangiectasia (HHT), and mice which lack expression of these receptors are embryonic lethal due to defects in angiogenesis. In addition, endoglin expression is potently up regulated during tumor-induced angiogenesis. In endothelial cells, TGF-ß signals through the type I TGF-ß receptor (ALK-5) or through ALK-1, to mediate opposing effects on endothelial cell proliferation and migration. However, the role of endoglin in regulating the balance in signaling between these pathways is unknown. Our laboratory has identified the nuclear hormone receptor, LXR-ß, as a protein that binds to activated ALK-1, is phosphorylated by ALK-1 and modulates ALK-1 signaling,establishing a novel signaling pathway downstream of ALK-1. Investigations in our laboratory have also revealed important functions for the cytoplasmic domain of endoglin, which is highly homologous to the cytoplasmic domain of the type III TGF-ß receptor. Studies are currently underway to further elucidate the signal transduction pathway downstream from these receptors and to establish their role in regulating tumor-induced angiogenesis. The ultimate goal of these studies is the ability to target the TGF-ß pathway for the chemoprevention or treatment of human cancers.
As endoglin and the type III TGF-ß receptors are both "co-receptors," a class of poorly understood cell surface receptors that bind ligand but are not thought to signal directly, another focus for the laboratory is establishing the role of these co-receptors in orchestrating signaling in physiological and pathophysiological settings.

In Their Words

Education and Training

  • Adult Oncology Fellow, Medicine, Dana Farber Cancer Institute, 1997 - 2000
  • Medical Resident, Medicine, Brigham and Women's Hospital, 1995 - 1997
  • Ph.D., Duke University, 1995
  • M.D., Duke University, 1995

Publications

Lee, NY, Haney, JC, Sogani, J, and Blobe, GC. "Casein kinase 2beta as a novel enhancer of activin-like receptor-1 signaling." FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, no. 11 (November 2009): 3712-3721.

PMID
19592636
Full Text

Mythreye, K, and Blobe, GC. "Proteoglycan signaling co-receptors: roles in cell adhesion, migration and invasion." Cell Signal 21, no. 11 (November 2009): 1548-1558. (Review)

PMID
19427900
Full Text

Mythreye, K, and Blobe, GC. "The type III TGFbeta receptor regulates directional migration: new tricks for an old dog." Cell Cycle 8, no. 19 (October 1, 2009): 3069-3070.

PMID
19755845
Full Text

Lee, NY, Kirkbride, KC, Sheu, RD, and Blobe, GC. "The transforming growth factor-beta type III receptor mediates distinct subcellular trafficking and downstream signaling of activin-like kinase (ALK)3 and ALK6 receptors." Mol Biol Cell 20, no. 20 (October 2009): 4362-4370.

PMID
19726563
Full Text

You, HJ, How, T, and Blobe, GC. "The type III transforming growth factor-beta receptor negatively regulates nuclear factor kappa B signaling through its interaction with beta-arrestin2." Carcinogenesis 30, no. 8 (August 2009): 1281-1287.

PMID
19325136
Full Text

Bullock, KE, Hurwitz, HI, Uronis, HE, Morse, MA, Blobe, GC, Hsu, SD, Zafar, SY, Nixon, AB, Howard, LA, and Bendell, JC. "Bevacizumab (B) plus everolimus (E) in refractory metastatic colorectal cancer (mCRC)." May 20, 2009.

Scholars@Duke

Howard, LA, Bullock, KE, Bendell, JC, Uronis, HE, Vlahovic, G, Blobe, GC, Riedel, RF, Nixon, AB, Gockerman, JP, and Hurwitz, HI. "Bevacizumab (B) plus everolimus (E) and panitumumab (P) in refractory advanced solid tumors." May 20, 2009.

Scholars@Duke

Mythreye, K, and Blobe, GC. "The type III TGF-beta receptor regulates epithelial and cancer cell migration through beta-arrestin2-mediated activation of Cdc42." Proc Natl Acad Sci U S A 106, no. 20 (May 19, 2009): 8221-8226.

PMID
19416857
Full Text

Howard, LA, Bullock, KE, Bendell, JC, Uronis, HE, Vlahovic, G, Blobe, GC, Riedel, RF, Nixon, AB, Gockerman, JP, and Hurwitz, HI. "Bevacizumab (B) plus everolimus (E) and panitumumab (P) in refractory advanced solid tumors." Journal of clinical oncology : official journal of the American Society of Clinical Oncology 27, no. 15_suppl (May 2009): 3551-.

PMID
27961368
Scholars@Duke

Bullock, KE, Hurwitz, HI, Uronis, HE, Morse, MA, Blobe, GC, Hsu, SD, Zafar, SY, Nixon, AB, Howard, LA, and Bendell, JC. "Bevacizumab (B) plus everolimus (E) in refractory metastatic colorectal cancer (mCRC)." Journal of clinical oncology : official journal of the American Society of Clinical Oncology 27, no. 15_suppl (May 2009): 4080-.

PMID
27961646
Scholars@Duke

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