Hua-Xin Liao, MD, PhD

Adjunct Professor in the Department of Medicine
Member of the Duke Human Vaccine Institute
Campus mail 4076/msrb-2, 2 Genome Court, Durham, NC 27710
Phone (919) 684-5858
Email address hliao@duke.edu

Dr. Liao is a Professor of Medicine and Research Director of Duke Human Vaccine Institute. Dr. Liao is a MD virologistt rained in China. In early 1980’s, Dr. Liao made major contributions to the first isolation of epidemic hemorrhagic fever virus (hataanvirus) from Apodemus agraius using tissue culture in China. The successful identification and isolation of Hataanvirus enabled the early diagnosis and treatment of the disease, and advancement of HFRS research towards prevention by development of a killed vaccine that has been currently used in China. Dr. Liao received his PhD from the UNC-CH in biochemistry in 1991, and his postdoctoral training on HIV-1 molecular biology and pathogenesis of HIV-1 at Duke University between 1991-1994, and joined the faculty at Duke University in 1994. Dr. Liao is an expert in medical virology, molecular biology and HIV-1 vaccine development.

Current research in Liao Lab focuses in the areas of: 1) HIV-1 vaccine immunogen design and development for induction of broadly reactive antibody and T cell responses; 2) host immune responses to HIV-1 infection; 3) host cellular factors that modulate HIV-host interaction, and 4) development of vaccines and detections for viral hemorrhagic fever and other emerging infectious diseases. Dr. Liao and his colleagues has recently developed a strategy by combining the isolation of Ig VH and VL genes from sorted single cells by RT-PCR and novel linear Ig heavy- and light-chain gene expression cassettes for high throughput sequence analysis and functional testing of Ig VH and VL genes expressed as recombinant antibodies. This novel approach would greatly facilitate study of B cell response and repertoires in acute HIV-1 infection as well as in other disease setting and development of therapeutic human monoclonal antibodies.

Key words: HIV-1, AIDS, HIV-1 vaccine, neutralizing antibody and viral hemorrhagic fever.

Education and Training

  • Ph.D., University of North Carolina at Chapel Hill, 1991
  • M.D., Shanghai Medical College Fudan University (China), 1977

Publications

Zhang, R, Alam, SM, Yu, JS, Scearce, R, Lockwood, B, Hwang, KK, Parks, R, Permar, S, Brandtzaeg, P, Haynes, BF, and Liao, HX. "Novel Monoclonal Antibodies for Studies of Human and Rhesus Macaque Secretory Component and Human J-Chain." Monoclonal antibodies in immunodiagnosis and immunotherapy 35, no. 4 (August 2016): 217-226.

PMID
27386924
Scholars@Duke

Li, H, Wang, S, Kong, R, Ding, W, Lee, FH, Parker, Z, Kim, E, Learn, GH, Hahn, P, Policicchio, B, Brocca-Cofano, E, Deleage, C, Hao, X, Chuang, GY, Gorman, J, Gardner, M, Lewis, MG, Hatziioannou, T, Santra, S, Apetrei, C, Pandrea, I, Alam, SM, Liao, HX, Shen, X, Tomaras, GD, Farzan, M, Chertova, E, Keele, BF, Estes, JD, Lifson, JD, Doms, RW, Montefiori, DC, Haynes, BF, Sodroski, JG, Kwong, PD, Hahn, BH, and Shaw, GM. "Envelope residue 375 substitutions in simian-human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques." Proceedings of the National Academy of Sciences of the United States of America 113, no. 24 (June 2016): E3413-E3422.

PMID
27247400
Scholars@Duke

Bushey, RT, Moody, MA, Nicely, NL, Haynes, BF, Alam, SM, Keir, ST, Bentley, RC, Roy Choudhury, K, Gottlin, EB, Campa, MJ, Liao, HX, and Patz, EF. "A Therapeutic Antibody for Cancer, Derived from Single Human B Cells." Cell reports 15, no. 7 (May 4, 2016): 1505-1513.

PMID
27160908
Scholars@Duke

Nelson, CS, Pollara, J, Kunz, EL, Jeffries, TL, Duffy, R, Beck, C, Stamper, L, Wang, M, Shen, X, Pickup, DJ, Staats, HF, Hudgens, MG, Kepler, TB, Montefiori, DC, Moody, MA, Tomaras, GD, Liao, HX, Haynes, BF, Ferrari, G, Fouda, GG, and Permar, SR. "Combined HIV-1 Envelope Systemic and Mucosal Immunization of Lactating Rhesus Monkeys Induces a Robust Immunoglobulin A Isotype B Cell Response in Breast Milk." Journal of virology 90, no. 10 (May 2016): 4951-4965.

PMID
26937027
Scholars@Duke

Madani, N, Princiotto, AM, Easterhoff, D, Bradley, T, Luo, K, Williams, WB, Liao, HX, Moody, MA, Phad, GE, Vázquez Bernat, N, Melillo, B, Santra, S, Smith, AB, Karlsson Hedestam, GB, Haynes, B, and Sodroski, J. "Antibodies Elicited by Multiple Envelope Glycoprotein Immunogens in Primates Neutralize Primary Human Immunodeficiency Viruses (HIV-1) Sensitized by CD4-Mimetic Compounds." Journal of virology 90, no. 10 (May 2016): 5031-5046.

PMID
26962221
Scholars@Duke

Bonsignori, M, Zhou, T, Sheng, Z, Chen, L, Gao, F, Joyce, MG, Ozorowski, G, Chuang, GY, Schramm, CA, Wiehe, K, Alam, SM, Bradley, T, Gladden, MA, Hwang, KK, Iyengar, S, Kumar, A, Lu, X, Luo, K, Mangiapani, MC, Parks, RJ, Song, H, Acharya, P, Bailer, RT, Cao, A, Druz, A, Georgiev, IS, Kwon, YD, Louder, MK, Zhang, B, Zheng, A, Hill, BJ, Kong, R, Soto, C, Mullikin, JC, Douek, DC, Montefiori, DC, Moody, MA, Shaw, GM, Hahn, BH, Kelsoe, G, Hraber, PT, Korber, BT, Boyd, SD, Fire, AZ, and Kepler, TB et al. "Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody." Cell 165, no. 2 (April 2016): 449-463.

PMID
26949186
Scholars@Duke

Zhang, R, Verkoczy, L, Wiehe, K, Munir Alam, S, Nicely, NI, Santra, S, Bradley, T, Pemble, CW, Zhang, J, Gao, F, Montefiori, DC, Bouton-Verville, H, Kelsoe, G, Larimore, K, Greenberg, PD, Parks, R, Foulger, A, Peel, JN, Luo, K, Lu, X, Trama, AM, Vandergrift, N, Tomaras, GD, Kepler, TB, Moody, MA, Liao, HX, and Haynes, BF. "Initiation of immune tolerance-controlled HIV gp41 neutralizing B cell lineages." Science translational medicine 8, no. 336 (April 2016): 336ra62-.

PMID
27122615
Scholars@Duke

Jeffries, TL, Sacha, CR, Pollara, J, Himes, J, Jaeger, FH, Dennison, SM, McGuire, E, Kunz, E, Eudailey, JA, Trama, AM, LaBranche, C, Fouda, GG, Wiehe, K, Montefiori, DC, Haynes, BF, Liao, HX, Ferrari, G, Alam, SM, Moody, MA, and Permar, SR. "The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells." Mucosal immunology 9, no. 2 (March 2016): 414-427.

PMID
26242599
Scholars@Duke

Wang, Y, Kapoor, P, Parks, R, Silva-Sanchez, A, Alam, SM, Verkoczy, L, Liao, HX, Zhuang, Y, Burrows, P, Levinson, M, Elgavish, A, Cui, X, Haynes, BF, and Schroeder, H. "HIV-1 gp140 epitope recognition is influenced by immunoglobulin DH gene segment sequence." Immunogenetics 68, no. 2 (February 2016): 145-155.

PMID
26687685
Scholars@Duke

Campa, MJ, Moody, MA, Zhang, R, Liao, HX, Gottlin, EB, and Patz, EF. "Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery." Cancer immunology, immunotherapy : CII 65, no. 2 (February 2016): 171-180.

PMID
26739486
Scholars@Duke

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