John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

McClellan, J. R., J. D. Hamilton, J. A. Alexander, W. G. Wolfe, and J. B. Reed. “Paecilomyces varioti endocarditis on a prosthetic aortic valve..” J Thorac Cardiovasc Surg 71, no. 3 (March 1976): 472–75.

PMID
943032
Scholars@Duke

Castelli, M. F., J. D. Hamilton, and A. Wynn-Williams. “Letter: Esophageal hemorrhage due to pancreatitis..” Can Med Assoc J 113, no. 7 (October 4, 1975): 605–6.

PMID
1181013
Scholars@Duke

Gallis, H. A., R. A. Berman, T. R. Cate, J. D. Hamilton, J. C. Gunnells, and D. L. Stickel. “Fungal infection following renal transplantation..” Arch Intern Med 135, no. 9 (September 1975): 1163–72.

PMID
1100007
Scholars@Duke

Hamilton, J. D., and D. M. Elliott. “Combined activity of amphotericin B and 5-fluorocytosine against Cryptococcus neoformans in vitro and in vivo in mice..” J Infect Dis 131, no. 2 (February 1975): 129–37. https://doi.org/10.1093/infdis/131.2.129.

PMID
1090685
Full Text

Hamilton, J. D., M. H. Hatch, and R. A. Gutman. “Serological evidence of cross infection in a dialysis unit hepatitis-B epidemic..” Kidney Int 6, no. 2 (August 1974): 118–21.

PMID
4214069
Scholars@Duke

Merchant, J. A., G. M. Halprin, A. R. Hudson, K. H. Kilburn, W. M. McKenzie, P. Bermanzohn, D. J. Hurst, J. D. Hamilton, and V. H. Germino. “Evaluation before and after exposure--the pattern of physiological response to cotton dust..” Ann N Y Acad Sci 221 (1974): 38–43. https://doi.org/10.1111/j.1749-6632.1974.tb28196.x.

PMID
4522561
Full Text

Feldman, H. A., J. D. Hamilton, and R. A. Gutman. “Amphotericin B therapy in an anephric patient..” Antimicrob Agents Chemother 4, no. 3 (September 1973): 302–5. https://doi.org/10.1128/aac.4.3.302.

PMID
4586143
Full Text

Merchant, J. A., J. C. Lumsden, K. H. Kilburn, V. H. Germino, J. D. Hamilton, W. S. Lynn, H. Byrd, and D. Baucom. “Preprocessing cotton to prevent byssinosis..” Br J Ind Med 30, no. 3 (July 1973): 237–47. https://doi.org/10.1136/oem.30.3.237.

PMID
4723787
Full Text

Merchant, J. A., J. C. Lumsden, K. H. Kilburn, W. M. O’Fallon, J. R. Ujda, V. H. Germino, and J. D. Hamilton. “Dose response studies in cotton textile workers..” J Occup Med 15, no. 3 (March 1973): 222–30.

PMID
4693165
Scholars@Duke

Pages