John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Champion, R., J. D. Hamilton, and M. Rodriguez-Garcia. “Clinicians' effectiveness in detecting patients' requests during an initial screening..” Hosp Community Psychiatry 40, no. 4 (April 1989): 413–15.

PMID
2714758
Scholars@Duke

Wray, N., B. Brody, T. Bayer, E. Boisaubin, F. Davila, R. Dresser, J. K. Dunn, H. T. Engelhardt, H. Haley, and J. D. Hamilton. “Withholding medical treatment from the severely demented patient. Decisional processes and cost implications..” Arch Intern Med 148, no. 9 (September 1988): 1980–84.

PMID
3137906
Scholars@Duke

Kaufman, A., J. D. Hamilton, and J. W. Peabody. “Medical education in China for the 21st century: the context for change..” Med Educ 22, no. 4 (July 1988): 253–60.

PMID
3173151
Scholars@Duke

Klotman, M. E., S. C. Henry, and J. D. Hamilton. “Determinants of the source of cytomegalovirus in murine renal allograft recipients..” Transplantation 44, no. 5 (November 1987): 636–39.

PMID
2825382
Scholars@Duke

Klotman, M. E., and J. D. Hamilton. “Cytomegalovirus pneumonia..” Semin Respir Infect 2, no. 2 (June 1987): 95–103.

PMID
2827281
Scholars@Duke

Hamilton, J. D., and W. Canteen. “Posttraumatic stress disorder in World War II naval veterans..” Hosp Community Psychiatry 38, no. 2 (February 1987): 197–99.

PMID
3557346
Scholars@Duke

Zimmerman, R. S., and J. D. Hamilton. “Yersinia enterocolitica inguinal lymphadenitis..” Diagn Microbiol Infect Dis 5, no. 3 (September 1986): 265–68.

PMID
3757476
Scholars@Duke

Drucker, J. L., D. J. Jacob, and J. D. Hamilton. “Delta hepatitis in North Carolina: learning from a patient..” N C Med J 47, no. 7 (July 1986): 339–40.

PMID
3461289
Scholars@Duke

Porter, K. R., D. M. Starnes, and J. D. Hamilton. “Reactivation of latent murine cytomegalovirus from kidney..” Kidney Int 28, no. 6 (December 1985): 922–25.

PMID
3003444
Scholars@Duke

Pages