John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Hamilton, J. D., J. F. Fitzwilliam, K. S. Cheung, J. Shelburne, D. J. Lang, and D. B. Amos. “Viral infection-homograft interactions in a murine model..” J Clin Invest 62, no. 6 (December 1978): 1303–12. https://doi.org/10.1172/JCI109251.

PMID
219027
Full Text

Marshall, J. G., and J. D. Hamilton. “The clinical librarian and the patient: report of a project at McMaster University Medical Centre..” Bull Med Libr Assoc 66, no. 4 (October 1978): 420–25.

PMID
708957
Scholars@Duke

Collins, S. M., J. D. Hamilton, T. D. Lewis, and I. Laufer. “Small-bowel malabsorption and gastrointestinal malignancy..” Radiology 126, no. 3 (March 1978): 603–9. https://doi.org/10.1148/126.3.603.

PMID
628726
Full Text

Goodacre, R. L., M. A. Ali, B. Vanderlinden, J. D. Hamilton, M. Castelli, and T. Seaton. “Hemolytic anemia in patients receiving sulfasalazine..” Digestion 17, no. 6 (1978): 503–8. https://doi.org/10.1159/000198156.

PMID
30666
Full Text

Ferrier, B. M., and J. D. Hamilton. “A preparatory course for medical students who lack a conventional academic background..” J Med Educ 52, no. 5 (May 1977): 390–95.

PMID
850255
Scholars@Duke

Hamilton, J. D., and E. W. Pfeiffer. “Effects of cold exposure and dehydration on renal function in black-tailed prairie dogs..” J Appl Physiol Respir Environ Exerc Physiol 42, no. 2 (February 1977): 295–99. https://doi.org/10.1152/jappl.1977.42.2.295.

PMID
838653
Full Text

Ali, M. A., E. J. Thomas, J. D. Hamilton, and M. C. Brain. “"Blood and guts": one component of an integrated program in biologic sciences as applied to medicine..” Can Med Assoc J 116, no. 1 (January 8, 1977): 59–62.

PMID
832221
Scholars@Duke

Goodacre, R. L., J. D. Hamilton, J. E. Mullens, and A. Qizilbash. “Persistence of proctitis in 2 cases of clindamycin-associated colitis..” Gastroenterology 72, no. 1 (January 1977): 149–52.

PMID
830228
Scholars@Duke

Hamilton, J. D., B. Larke, and A. Qizilbash. “Transmission of hepatitis B by a human bite: an occupational hazard..” Can Med Assoc J 115, no. 5 (September 4, 1976): 439–40.

PMID
953919
Scholars@Duke

Hamilton, J. D., R. A. Chambers, and A. Wynn-Williams. “Role of gluten, prednisone, and azathioprine in non-responsive coeliac disease..” Lancet 1, no. 7971 (June 5, 1976): 1213–16. https://doi.org/10.1016/s0140-6736(76)92162-0.

PMID
58260
Full Text

Pages