John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Arif, S, and Perfect, JR. "Emergence of the Molds Other than Aspergillus in Immunocompromised Patients." Clinics in chest medicine 38, no. 3 (September 2017): 555-573. (Review)

PMID
28797495
Full Text

Rhodes, J, Desjardins, CA, Sykes, SM, Beale, MA, Vanhove, M, Sakthikumar, S, Chen, Y, Gujja, S, Saif, S, Chowdhary, A, Lawson, DJ, Ponzio, V, Colombo, AL, Meyer, W, Engelthaler, DM, Hagen, F, Illnait-Zaragozi, MT, Alanio, A, Vreulink, J-M, Heitman, J, Perfect, JR, Litvintseva, AP, Bicanic, T, Harrison, TS, Fisher, MC, and Cuomo, CA. "Tracing Genetic Exchange and Biogeography of Cryptococcus neoformans var. grubii at the Global Population Level." Genetics 207, no. 1 (September 2017): 327-346.

PMID
28679543
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Alvarez, MI, Glover, LC, Luo, P, Wang, L, Theusch, E, Oehlers, SH, Walton, EM, Tram, TTB, Kuang, Y-L, Rotter, JI, McClean, CM, Chinh, NT, Medina, MW, Tobin, DM, Dunstan, SJ, and Ko, DC. "Human genetic variation in VAC14 regulates Salmonella invasion and typhoid fever through modulation of cholesterol." Proceedings of the National Academy of Sciences of the United States of America 114, no. 37 (September 2017): E7746-E7755.

PMID
28827342
Full Text

Perfect, JR. "The antifungal pipeline: a reality check." Nature Reviews. Drug Discovery 16, no. 9 (September 2017): 603-616. (Review)

PMID
28496146
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McCarthy, MW, Kontoyiannis, DP, Cornely, OA, Perfect, JR, and Walsh, TJ. "Novel Agents and Drug Targets to Meet the Challenges of Resistant Fungi." The Journal of Infectious Diseases 216, no. suppl_3 (August 2017): S474-S483. (Review)

PMID
28911042
Full Text

Miao, Y, Tenor, JL, Toffaletti, DL, Maskarinec, SA, Liu, J, Lee, RE, Perfect, JR, and Brennan, RG. "Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design." Mbio 8, no. 4 (July 25, 2017).

PMID
28743811
Full Text

Desjardins, CA, Giamberardino, C, Sykes, SM, Yu, C-H, Tenor, JL, Chen, Y, Yang, T, Jones, AM, Sun, S, Haverkamp, MR, Heitman, J, Litvintseva, AP, Perfect, JR, and Cuomo, CA. "Population genomics and the evolution of virulence in the fungal pathogen Cryptococcus neoformans." Genome Research 27, no. 7 (July 2017): 1207-1219.

PMID
28611159
Full Text

Thammahong, A, Puttikamonkul, S, Perfect, JR, Brennan, RG, and Cramer, RA. "Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development." Microbiology and molecular biology reviews : MMBR 81, no. 2 (June 2017). (Review)

PMID
28298477
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Lestner, J, McEntee, L, Johnson, A, Livermore, J, Whalley, S, Schwartz, J, Perfect, JR, Harrison, T, and Hope, W. "Experimental Models of Short Courses of Liposomal Amphotericin B for Induction Therapy for Cryptococcal Meningitis." Antimicrobial Agents and Chemotherapy 61, no. 6 (June 2017).

PMID
28320715
Full Text

Köhler, JR, Hube, B, Puccia, R, Casadevall, A, and Perfect, JR. "Fungi that Infect Humans." Microbiology Spectrum 5, no. 3 (June 2017). (Review)

PMID
28597822
Full Text

Pages