John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Thompson, GR, Rendon, A, Ribeiro Dos Santos, R, Queiroz-Telles, F, Ostrosky-Zeichner, L, Azie, N, Maher, R, Lee, M, Kovanda, L, Engelhardt, M, Vazquez, JA, Cornely, OA, and Perfect, JR. "Isavuconazole Treatment of Cryptococcosis and Dimorphic Mycoses." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 63, no. 3 (August 2016): 356-362.

PMID
27169478
Full Text

Marty, FM, Ostrosky-Zeichner, L, Cornely, OA, Mullane, KM, Perfect, JR, Thompson, GR, Alangaden, GJ, Brown, JM, Fredricks, DN, Heinz, WJ, Herbrecht, R, Klimko, N, Klyasova, G, Maertens, JA, Melinkeri, SR, Oren, I, Pappas, PG, Ráčil, Z, Rahav, G, Santos, R, Schwartz, S, Vehreschild, JJ, Young, J-AH, Chetchotisakd, P, Jaruratanasirikul, S, Kanj, SS, Engelhardt, M, Kaufhold, A, Ito, M, Lee, M, Sasse, C, Maher, RM, Zeiher, B, Vehreschild, MJGT, and VITAL and FungiScope Mucormycosis Investigators, . "Isavuconazole treatment for mucormycosis: a single-arm open-label trial and case-control analysis." The Lancet. Infectious diseases 16, no. 7 (July 2016): 828-837.

PMID
26969258
Full Text

Perfect, JR. ""Is there an emerging need for new antifungals?"." Expert opinion on emerging drugs 21, no. 2 (June 2016): 129-131.

PMID
26883732
Full Text

Perfect, JR. "Editorial Commentary: Life-saving Antimicrobial Drugs: What Are We Doing to Pricing and Availability?." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 62, no. 12 (June 2016): 1569-1570.

PMID
27009252
Full Text

Homer, CM, Summers, DK, Goranov, AI, Clarke, SC, Wiesner, DL, Diedrich, JK, Moresco, JJ, Toffaletti, D, Upadhya, R, Caradonna, I, Petnic, S, Pessino, V, Cuomo, CA, Lodge, JK, Perfect, J, Yates, JR, Nielsen, K, Craik, CS, and Madhani, HD. "Intracellular Action of a Secreted Peptide Required for Fungal Virulence." Cell host & microbe 19, no. 6 (June 2016): 849-864.

PMID
27212659
Full Text

Maziarz, EK, and Perfect, JR. "Cryptococcosis." Infectious disease clinics of North America 30, no. 1 (March 2016): 179-206. (Review)

PMID
26897067
Full Text

Swamydas, M, Gao, J-L, Break, TJ, Johnson, MD, Jaeger, M, Rodriguez, CA, Lim, JK, Green, NM, Collar, AL, Fischer, BG, Lee, C-CR, Perfect, JR, Alexander, BD, Kullberg, B-J, Netea, MG, Murphy, PM, and Lionakis, MS. "CXCR1-mediated neutrophil degranulation and fungal killing promote Candida clearance and host survival." Science translational medicine 8, no. 322 (January 2016): 322ra10-.

PMID
26791948
Full Text

Janout, V, Bienvenu, C, Schell, W, Perfect, JR, and Regen, SL. "Correction to Molecular Umbrella-Amphotericin B Conjugates." Bioconjugate chemistry 26, no. 10 (October 2015): 2168-.

PMID
26421479
Full Text

Janout, V, Schell, WA, Thévenin, D, Yu, Y, Perfect, JR, and Regen, SL. "Taming Amphotericin B." Bioconjugate chemistry 26, no. 10 (October 2015): 2021-2024.

PMID
26340430
Full Text

Tenor, JL, Oehlers, SH, Yang, JL, Tobin, DM, and Perfect, JR. "Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion." mBio 6, no. 5 (September 29, 2015): e01425-e01415.

PMID
26419880
Full Text

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