John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Pound, MW, Drew, RH, and Perfect, JR. "Recent advances in the epidemiology, prevention, diagnosis, and treatment of fungal pneumonia." Curr Opin Infect Dis 15, no. 2 (April 2002): 183-194. (Review)

PMID
11964921
Scholars@Duke

Wang, P, Nichols, CB, Lengeler, KB, Cardenas, ME, Cox, GM, Perfect, JR, and Heitman, J. "Mating-type-specific and nonspecific PAK kinases play shared and divergent roles in Cryptococcus neoformans." Eukaryotic Cell 1, no. 2 (April 2002): 257-272.

PMID
12455960
Full Text

Walsh, TJ, Lutsar, I, Driscoll, T, Dupont, B, Roden, M, Ghahramani, P, Hodges, M, Groll, AH, and Perfect, JR. "Voriconazole in the treatment of aspergillosis, scedosporiosis and other invasive fungal infections in children." Pediatr Infect Dis J 21, no. 3 (March 2002): 240-248.

PMID
12005089
Scholars@Duke

Cruz, MC, Goldstein, AL, Blankenship, JR, Del Poeta, M, Davis, D, Cardenas, ME, Perfect, JR, McCusker, JH, and Heitman, J. "Calcineurin is essential for survival during membrane stress in Candida albicans." The Embo Journal 21, no. 4 (February 2002): 546-559.

PMID
11847103
Full Text

Schiappa, D, Gueyikian, A, Kakar, S, Alspaugh, JA, Perfect, JR, and Williamson, PR. "An auxotrophic pigmented Cryptococcus neoformans strain causing infection of the bone marrow." Med Mycol 40, no. 1 (February 2002): 1-5.

PMID
11860008
Scholars@Duke

Alspaugh, JA, Pukkila-Worley, R, Harashima, T, Cavallo, LM, Funnell, D, Cox, GM, Perfect, JR, Kronstad, JW, and Heitman, J. "Adenylyl cyclase functions downstream of the Galpha protein Gpa1 and controls mating and pathogenicity of Cryptococcus neoformans." Eukaryotic Cell 1, no. 1 (February 2002): 75-84.

PMID
12455973
Full Text

Hadjiliadis, D, Sporn, TA, Perfect, JR, Tapson, VF, Davis, RD, and Palmer, SM. "Outcome of lung transplantation in patients with mycetomas." Chest 121, no. 1 (January 2002): 128-134.

PMID
11796441
Scholars@Duke

Gorlach, JM, McDade, HC, Perfect, JR, and Cox, GM. "Antisense repression in Cryptococcus neoformans as a laboratory tool and potential antifungal strategy." Microbiology (Reading, England) 148, no. Pt 1 (January 2002): 213-219.

PMID
11782513
Full Text

Walsh, TJ, Pappas, P, Winston, DJ, Lazarus, HM, Petersen, F, Raffalli, J, Yanovich, S, Stiff, P, Greenberg, R, Donowitz, G, Schuster, M, Reboli, A, Wingard, J, Arndt, C, Reinhardt, J, Hadley, S, Finberg, R, Laverdière, M, Perfect, J, Garber, G, Fioritoni, G, Anaissie, E, Lee, J, and National Institute of Allergy and Infectious Diseases Mycoses Study Group, . "Voriconazole compared with liposomal amphotericin B for empirical antifungal therapy in patients with neutropenia and persistent fever." The New England Journal of Medicine 346, no. 4 (January 2002): 225-234.

PMID
11807146
Full Text

Perfect, JR. "New antifungal agents." Transpl Infect Dis 4 Suppl 3 (2002): 52-61. (Review)

PMID
12486793
Scholars@Duke

Pages