John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Lu, I, Dodds, E, and Perfect, J. "New antifungal agents." Seminars in Respiratory Infections 17, no. 2 (2002): 140-150.

PMID
12070833
Full Text

Gorlach, JM, McDade, HC, Perfect, JR, and Cox, GM. "Antisense repression in Cryptococcus neoformans as a laboratory tool and potential antifungal strategy." Microbiology 148, no. 1 (2002): 213-219.

Scholars@Duke

Loeffler, AM, Drew, RH, Perfect, JR, Grethe, NI, Stephens, JW, Gray, SL, and Talbot, GH. "Safety and efficacy of quinupristin/dalfopristin for treatment of invasive Gram-positive infections in pediatric patients." Pediatric Infectious Disease Journal 21, no. 10 (2002): 950-956.

Full Text

Perfect, JR, Cox, GM, Lee, JY, Kauffman, CA, de Repentigny, L, Chapman, SW, Morrison, VA, Pappas, P, Hiemenz, JW, Stevens, DA, and Mycoses Study Group, . "The impact of culture isolation of Aspergillus species: a hospital-based survey of aspergillosis." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America 33, no. 11 (December 2001): 1824-1833.

PMID
11692293
Full Text

Cruz, MC, Goldstein, AL, Blankenship, J, Del Poeta, M, Perfect, JR, McCusker, JH, Bennani, YL, Cardenas, ME, and Heitman, J. "Rapamycin and less immunosuppressive analogs are toxic to Candida albicans and Cryptococcus neoformans via FKBP12-dependent inhibition of TOR." Antimicrobial Agents and Chemotherapy 45, no. 11 (November 2001): 3162-3170.

PMID
11600372
Full Text

Gottfredsson, M, Jessup, CJ, Cox, GM, Perfect, JR, and Ghannoum, MA. "Fungal phospholipase activity and susceptibility to lipid preparations of amphotericin B." Antimicrobial Agents and Chemotherapy 45, no. 11 (November 2001): 3231-3233.

PMID
11600388
Full Text

Johnson, MD, and Perfect, JR. "Fungal Infections of the Bones and Joints." Curr Infect Dis Rep 3, no. 5 (October 2001): 450-460.

PMID
11559466
Scholars@Duke

Pappas, PG, Perfect, JR, Cloud, GA, Larsen, RA, Pankey, GA, Lancaster, DJ, Henderson, H, Kauffman, CA, Haas, DW, Saccente, M, Hamill, RJ, Holloway, MS, Warren, RM, and Dismukes, WE. "Cryptococcosis in human immunodeficiency virus-negative patients in the era of effective azole therapy." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America 33, no. 5 (September 2001): 690-699.

PMID
11477526
Full Text

Palmer, SM, Drew, RH, Whitehouse, JD, Tapson, VF, Davis, RD, McConnell, RR, Kanj, SS, and Perfect, JR. "Safety of aerosolized amphotericin B lipid complex in lung transplant recipients." Transplantation 72, no. 3 (August 15, 2001): 545-548.

PMID
11502995
Scholars@Duke

Wang, P, Cardenas, ME, Cox, GM, Perfect, JR, and Heitman, J. "Two cyclophilin A homologs with shared and distinct functions important for growth and virulence of Cryptococcus neoformans." Embo Reports 2, no. 6 (June 2001): 511-518.

PMID
11415984
Full Text

Pages