John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Wills, EA, Redinbo, MR, Perfect, JR, and Poeta, MD. "New potential targets for antifungal development." Expert Opinion on Therapeutic Targets 4, no. 3 (2000): 265-296.

Full Text

Perfect, JR, and Cox, GM. "Virulence mechanisms for fungi, Part II." Clinical Microbiology Newsletter 22, no. 16 (2000): 121-125.

Full Text

Perfect, JR, and Cox, GM. "Virulence mechanisms for fungi, Part I." Clinical Microbiology Newsletter 22, no. 15 (2000): 113-119.

Full Text

Klein, RS, Arnsten, JH, Sobel, JD, Gottfredsson, M, Cox, GM, and Perfect, JR. "Oropharyngeal Candida colonization and human immunodeficiency virus type 1 infection [8] (multiple letters)." Journal of Infectious Diseases 181, no. 2 (2000): 812-813.

PMID
10669393
Scholars@Duke

Dodds, ES, Drew, RH, and Perfect, JR. "Antifungal pharmacodynamics: Review of the literature and clinical applications." Pharmacotherapy 20, no. 11 I (2000): 1335-1355.

Scholars@Duke

Drew, RH, Perfect, JR, Srinath, L, Kurkimilis, E, Dowzicky, M, and Talbot, GH. "Treatment of methicillin-resistant Staphylococcus aureus infections with quinupristin-dalfopristin in patients intolerant of or failing prior therapy." Journal of Antimicrobial Chemotherapy 46, no. 5 (2000): 775-784.

Scholars@Duke

Nichols, WG, McCann, RL, and Perfect, JR. "Tuberculous pseudoaneurysm of the thoracoabdominal aorta: Case and review." Infectious Diseases in Clinical Practice 9, no. 1 (2000): 3-9.

Scholars@Duke

Deepe, GS, Romani, L, Calich, VL, Huffnagle, G, Arruda, C, Molinari-Madlum, EE, and Perfect, JR. "Knockout mice as experimental models of virulence." Med Mycol 38 Suppl 1 (2000): 87-98. (Review)

PMID
11204168
Scholars@Duke

Gottfredsson, M, and Perfect, JR. "Fungal meningitis." Semin Neurol 20, no. 3 (2000): 307-322. (Review)

PMID
11051295
Full Text

Zallen, JA, Peckol, EL, Tobin, DM, and Bargmann, CI. "Neuronal cell shape and neurite initiation are regulated by the Ndr kinase SAX-1, a member of the Orb6/COT-1/warts serine/threonine kinase family." Molecular Biology of the Cell 11, no. 9 (2000): 3177-3190.

PMID
10982409
Scholars@Duke

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