John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Wills, EA, Roberts, IS, Del Poeta, M, Rivera, J, Casadevall, A, Cox, GM, and Perfect, JR. "Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity." Molecular Microbiology 40, no. 3 (May 2001): 610-620.

PMID
11359567
Full Text

D'Souza, CA, Alspaugh, JA, Yue, C, Harashima, T, Cox, GM, Perfect, JR, and Heitman, J. "Cyclic AMP-dependent protein kinase controls virulence of the fungal pathogen Cryptococcus neoformans." Molecular and Cellular Biology 21, no. 9 (May 2001): 3179-3191.

PMID
11287622
Full Text

Stephens, CE, Tanious, F, Kim, S, Wilson, WD, Schell, WA, Perfect, JR, Franzblau, SG, and Boykin, DW. "Diguanidino and "reversed" diamidino 2,5-diarylfurans as antimicrobial agents." Journal of Medicinal Chemistry 44, no. 11 (May 2001): 1741-1748.

PMID
11356109
Full Text

Hu, G, Yi, S, Roberts, BC, Hayes, CE, Schell, WA, Perfect, JR, and Fowler, WC. "In vitro susceptibilities of ocular isolates in fungal keratitis to posaconazole, a new triazole antifungal agent." INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 42, no. 4 (March 15, 2001): S580-S580.

Scholars@Duke

PERFECT, J. "The impact of culture isolation of Aspergillus species : a hospital-based survey of aspergillosis." Clin Infect Dis 33 (2001): 1824-1833.

Full Text

Cox, GM, McDade, HC, Chen, SC, Tucker, SC, Gottfredsson, M, Wright, LC, Sorrell, TC, Leidich, SD, Casadevall, A, Ghannoum, MA, and Perfect, JR. "Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans." Molecular Microbiology 39, no. 1 (January 2001): 166-175.

PMID
11123698
Full Text

Luberto, C, Toffaletti, DL, Wills, EA, Tucker, SC, Casadevall, A, Perfect, JR, Hannun, YA, and Del Poeta, M. "Roles for inositol-phosphoryl ceramide synthase 1 (IPC1) in pathogenesis of C. neoformans." Genes & Development 15, no. 2 (January 2001): 201-212.

PMID
11157776
Full Text

Lengeler, KB, Wang, P, Cox, GM, Perfect, JR, and Heitman, J. "Identification of the MATa mating-type locus of Cryptococcus neoformans reveals a serotype A MATa strain thought to have been extinct." Proceedings of the National Academy of Sciences of the United States of America 97, no. 26 (December 2000): 14455-14460.

PMID
11121047
Full Text

Drew, RH, Perfect, JR, Srinath, L, Kurkimilis, E, Dowzicky, M, and Talbot, GH. "Treatment of methicillin-resistant staphylococcus aureus infections with quinupristin-dalfopristin in patients intolerant of or failing prior therapy. For the Synercid Emergency-Use Study Group." The Journal of Antimicrobial Chemotherapy 46, no. 5 (November 2000): 775-784.

PMID
11062197
Full Text

Dodds, ES, Drew, RH, and Perfect, JR. "Antifungal pharmacodynamics: review of the literature and clinical applications." Pharmacotherapy 20, no. 11 (November 2000): 1335-1355. (Review)

PMID
11079283
Full Text

Pages