John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Cruz, MC, Cavallo, LM, Görlach, JM, Cox, G, Perfect, JR, Cardenas, ME, and Heitman, J. "Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans." Molecular and Cellular Biology 19, no. 6 (June 1999): 4101-4112.

PMID
10330150
Full Text

Del Poeta, M, Toffaletti, DL, Rude, TH, Dykstra, CC, Heitman, J, and Perfect, JR. "Topoisomerase I is essential in Cryptococcus neoformans: role In pathobiology and as an antifungal target." Genetics 152, no. 1 (May 1999): 167-178.

PMID
10224251
Scholars@Duke

Asai, K, Tsuchimori, N, Okonogi, K, Perfect, JR, Gotoh, O, and Yoshida, Y. "Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450." Antimicrobial Agents and Chemotherapy 43, no. 5 (May 1999): 1163-1169.

PMID
10223930
Full Text

del Poeta, M, Toffaletti, DL, Rude, TH, Sparks, SD, Heitman, J, and Perfect, JR. "Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein." Infection and Immunity 67, no. 4 (April 1999): 1812-1820.

PMID
10085022
Scholars@Duke

Gottfredsson, M, and Perfect, JR. "Use of antifungal agents in the intensive care unit." Current Opinion in Critical Care 5, no. 5 (January 1, 1999): 381-390.

Full Text

Heitman, J, Casadevall, A, Lodge, JK, and Perfect, JR. "The Cryptococcus neoformans genome sequencing project." Mycopathologia 148, no. 1 (January 1999): 1-7. (Review)

PMID
11086479
Full Text

Thompson, JR, Douglas, CM, Li, W, Jue, CK, Pramanik, B, Yuan, X, Rude, TH, Toffaletti, DL, Perfect, JR, and Kurtz, M. "A glucan synthase FKS1 homolog in cryptococcus neoformans is single copy and encodes an essential function." Journal of Bacteriology 181, no. 2 (January 1999): 444-453.

PMID
9882657
Scholars@Duke

Perfect, JR, Wong, B, Chang, YC, Kwon-Chung, KJ, and Williamson, PR. "Cryptococcus neoformans: Virulence and host defences." Journal of Medical and Veterinary Mycology 3 (December 1, 1998): 79-86.

Scholars@Duke

Gottfredsson, M, Cox, GM, and Perfect, JR. "Molecular methods for epidemiological and diagnostic studies of fungal infections." Pathology 30, no. 4 (November 1998): 405-418. (Review)

PMID
9839319
Full Text

Alspaugh, JA, Perfect, JR, and Heitman, J. "Signal transduction pathways regulating differentiation and pathogenicity of Cryptococcus neoformans." Fungal Genet Biol 25, no. 1 (October 1998): 1-14. (Review)

PMID
9806801
Full Text

Pages