John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

PERFECT, J. "The impact of culture isolation of Aspergillus species : a hospital-based survey of aspergillosis." Clin Infect Dis 33 (2001): 1824-1833.

Full Text

Cox, GM, McDade, HC, Chen, SC, Tucker, SC, Gottfredsson, M, Wright, LC, Sorrell, TC, Leidich, SD, Casadevall, A, Ghannoum, MA, and Perfect, JR. "Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans." Molecular Microbiology 39, no. 1 (January 2001): 166-175.

PMID
11123698
Full Text

Lengeler, KB, Wang, P, Cox, GM, Perfect, JR, and Heitman, J. "Identification of the MATa mating-type locus of Cryptococcus neoformans reveals a serotype A MATa strain thought to have been extinct." Proceedings of the National Academy of Sciences of the United States of America 97, no. 26 (December 2000): 14455-14460.

PMID
11121047
Full Text

Drew, RH, Perfect, JR, Srinath, L, Kurkimilis, E, Dowzicky, M, and Talbot, GH. "Treatment of methicillin-resistant staphylococcus aureus infections with quinupristin-dalfopristin in patients intolerant of or failing prior therapy. For the Synercid Emergency-Use Study Group." The Journal of Antimicrobial Chemotherapy 46, no. 5 (November 2000): 775-784.

PMID
11062197
Full Text

Dodds, ES, Drew, RH, and Perfect, JR. "Antifungal pharmacodynamics: review of the literature and clinical applications." Pharmacotherapy 20, no. 11 (November 2000): 1335-1355. (Review)

PMID
11079283
Full Text

Zallen, JA, Peckol, EL, Tobin, DM, and Bargmann, CI. "Neuronal cell shape and neurite initiation are regulated by the Ndr kinase SAX-1, a member of the Orb6/COT-1/warts serine/threonine kinase family." Molecular Biology of the Cell 11, no. 9 (September 2000): 3177-3190.

PMID
10982409
Full Text

Görlach, J, Fox, DS, Cutler, NS, Cox, GM, Perfect, JR, and Heitman, J. "Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans." The Embo Journal 19, no. 14 (July 2000): 3618-3629.

PMID
10899116
Full Text

Omer, I, Perfect, JR, Warren, RM, Hamill, RJ, Kauffman, CA, and Pappas, PG. "Cryptococcosis without central nervous system or pulmonary involvement in HIV-negative patients." July 2000.

Scholars@Duke

Perfect, JR, Lutsar, I, and Gonzalez-Ruiz, A. "Voriconazole (VORI) for the treatment of resistant and rare fungal pathogens." July 2000.

Scholars@Duke

Schlamm, HT, Corey, L, Brown, J, and Perfect, JR. "Voriconazole for salvage treatment of invasive aspergillosis." July 2000.

Scholars@Duke

Pages