John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Asai, K, Tsuchimori, N, Okonogi, K, Perfect, JR, Gotoh, O, and Yoshida, Y. "Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450." Antimicrob Agents Chemother 43, no. 5 (May 1999): 1163-1169.

PMID
10223930
Scholars@Duke

del Poeta, M, Toffaletti, DL, Rude, TH, Sparks, SD, Heitman, J, and Perfect, JR. "Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein." Infect Immun 67, no. 4 (April 1999): 1812-1820.

PMID
10085022
Scholars@Duke

Thompson, JR, Douglas, CM, Li, W, Jue, CK, Pramanik, B, Yuan, X, Rude, TH, Toffaletti, DL, Perfect, JR, and Kurtz, M. "A glucan synthase FKS1 homolog in cryptococcus neoformans is single copy and encodes an essential function." J Bacteriol 181, no. 2 (January 1999): 444-453.

PMID
9882657
Scholars@Duke

Heitman, J, Casadevall, A, Lodge, JK, and Perfect, JR. "The Cryptococcus neoformans genome sequencing project." Mycopathologia 148, no. 1 (1999): 1-7. (Review)

PMID
11086479
Scholars@Duke

Gottfredsson, M, and Perfect, JR. "Use of antifungal agents in the intensive care unit." Current Opinion in Critical Care 5, no. 5 (1999): 381-390.

Scholars@Duke

Gottfredsson, M, Cox, GM, and Perfect, JR. "Molecular methods for epidemiological and diagnostic studies of fungal infections." Pathology 30, no. 4 (November 1998): 405-418. (Review)

PMID
9839319
Scholars@Duke

Schell, WA, De Almeida, GM, Dodge, RK, Okonogi, K, and Perfect, JR. "In vitro and in vivo efficacy of the triazole TAK-187 against Cryptococcus neoformans." Antimicrob Agents Chemother 42, no. 10 (October 1998): 2630-2632.

PMID
9756767
Scholars@Duke

Del Poeta, M, Schell, WA, Dykstra, CC, Jones, S, Tidwell, RR, Czarny, A, Bajic, M, Kumar, A, Boykin, D, and Perfect, JR. "Structure-in vitro activity relationships of pentamidine analogues and dication-substituted bis-benzimidazoles as new antifungal agents." Antimicrob Agents Chemother 42, no. 10 (October 1998): 2495-2502.

PMID
9756747
Scholars@Duke

Del Poeta, M, Schell, WA, Dykstra, CC, Jones, SK, Tidwell, RR, Kumar, A, Boykin, DW, and Perfect, JR. "In vitro antifungal activities of a series of dication-substituted carbazoles, furans, and benzimidazoles." Antimicrob Agents Chemother 42, no. 10 (October 1998): 2503-2510.

PMID
9756748
Scholars@Duke

Alspaugh, JA, Perfect, JR, and Heitman, J. "Signal transduction pathways regulating differentiation and pathogenicity of Cryptococcus neoformans." Fungal Genet Biol 25, no. 1 (October 1998): 1-14. (Review)

PMID
9806801
Full Text

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