John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Saag, MS, Graybill, RJ, Larsen, RA, Pappas, PG, Perfect, JR, Powderly, WG, Sobel, JD, and Dismukes, WE. "Practice guidelines for the management of cryptococcal disease. Infectious Diseases Society of America." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America 30, no. 4 (April 20, 2000): 710-718.

PMID
10770733
Full Text

Alspaugh, JA, Cavallo, LM, Perfect, JR, and Heitman, J. "RAS1 regulates filamentation, mating and growth at high temperature of Cryptococcus neoformans." Molecular Microbiology 36, no. 2 (April 2000): 352-365.

PMID
10792722
Full Text

Luna, B, Drew, RH, and Perfect, JR. "Agents for treatment of invasive fungal infections." Otolaryngol Clin North Am 33, no. 2 (April 2000): 277-299. (Review)

PMID
10736404
Scholars@Duke

Del Poeta, M, Cruz, MC, Cardenas, ME, Perfect, JR, and Heitman, J. "Synergistic antifungal activities of bafilomycin A(1), fluconazole, and the pneumocandin MK-0991/caspofungin acetate (L-743,873) with calcineurin inhibitors FK506 and L-685,818 against Cryptococcus neoformans." Antimicrobial Agents and Chemotherapy 44, no. 3 (March 2000): 739-746.

PMID
10681348
Full Text

Cox, GM, Mukherjee, J, Cole, GT, Casadevall, A, and Perfect, JR. "Urease as a virulence factor in experimental cryptococcosis." Infection and Immunity 68, no. 2 (February 2000): 443-448.

PMID
10639402
Full Text

Gottfredsson, M, Cox, GM, and Perfect, JR. "Reply." J Infect Dis 181, no. 2 (February 2000): 812-813.

PMID
10669394
Full Text

Cruz, MC, Del Poeta, M, Wang, P, Wenger, R, Zenke, G, Quesniaux, VF, Movva, NR, Perfect, JR, Cardenas, ME, and Heitman, J. "Immunosuppressive and nonimmunosuppressive cyclosporine analogs are toxic to the opportunistic fungal pathogen Cryptococcus neoformans via cyclophilin-dependent inhibition of calcineurin." Antimicrobial Agents and Chemotherapy 44, no. 1 (January 2000): 143-149.

PMID
10602736
Full Text

Wang, P, Perfect, JR, and Heitman, J. "The G-protein beta subunit GPB1 is required for mating and haploid fruiting in Cryptococcus neoformans." Molecular and Cellular Biology 20, no. 1 (January 2000): 352-362.

PMID
10594037
Full Text

Deepe, GS, Romani, L, Calich, VL, Huffnagle, G, Arruda, C, Molinari-Madlum, EE, and Perfect, JR. "Knockout mice as experimental models of virulence." Medical Mycology 38 Suppl 1 (January 2000): 87-98. (Review)

PMID
11204168
Full Text

Gottfredsson, M, and Perfect, JR. "Fungal meningitis." Seminars in Neurology 20, no. 3 (January 2000): 307-322. (Review)

PMID
11051295
Full Text

Pages