John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Durack, D, Perfect, J, Hess, J, and Dankert, J. "Tolerance in viridans streptococci exerts a major influence on efficacy of penicillin in vivo." Clinical Research 30, no. 2 (1982): 364A-.

Scholars@Duke

Perfect, JR, Lang, SD, and Durack, DT. "Influence of agglutinating antibody in experimental cryptococcal meningitis." Br J Exp Pathol 62, no. 6 (December 1981): 595-599.

PMID
7034765
Scholars@Duke

Perfect, JR, and Durack, DT. "Pharmacokinetics of cefoperazone, moxalactam, cefotaxime, trimethoprim and sulphamethoxazole in experimental meningitis." J Antimicrob Chemother 8, no. 1 (July 1981): 49-58.

PMID
6265429
Scholars@Duke

Durack, DT, and Perfect, JR. "Potential value of cefoperazone in bacterial meningitis: experimental studies." Drugs 22 Suppl 1 (1981): 60-64.

PMID
6269824
Scholars@Duke

Perfect, JR. "Mediastinitis complicating cellulitis of the head and neck." South Med J 73, no. 10 (October 1980): 1378-1380.

PMID
7434054
Scholars@Duke

Perfect, JR, Lang, SD, and Durack, DT. "Chronic cryptococcal meningitis: a new experimental model in rabbits." Am J Pathol 101, no. 1 (October 1980): 177-194.

PMID
7004196
Scholars@Duke

Perfect, JR, Lang, SD, and Durack, DT. "Comparison of cotrimoxazole, ampicillin, and chloramphenicol in treatment of experimental Haemophilus influenzae type B meningitis." Antimicrob Agents Chemother 17, no. 1 (January 1980): 43-48.

PMID
6965442
Scholars@Duke

Perfect, JR, Lang, SDR, and Durack, DT. "Comparison of cotrimoxazole (TMP/SMX), ampicillin (AMP) and chloramphenicol (CHLORO) in treatment of experimental Haemophilus influenzae type B (HITB) meningitis." Clinical Research 27, no. 2 (1979): 353A-.

Scholars@Duke

Pitman, SK, Drew, RH, and Perfect, JR. "Addressing current medical needs in invasive fungal infection prevention and treatment with new antifungal agents, strategies and formulations." Expert Opin Emerg Drugs 16, no. 3: 559-586.

PMID
21846302
Full Text

Pages