John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Lodge, J. K., E. Jackson-Machelski, D. L. Toffaletti, J. R. Perfect, and J. I. Gordon. “Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans..” Proc Natl Acad Sci U S A 91, no. 25 (December 6, 1994): 12008–12. https://doi.org/10.1073/pnas.91.25.12008.

PMID
7991574
Full Text

DENNING, D. W., J. Y. LEE, J. S. HOSTETLER, P. PAPPAS, C. A. KAUFFMAN, D. H. DEWSNUP, J. N. GALGIANI, et al. “NIAID MYCOSES STUDY-GROUP MULTICENTER TRIAL OF ORAL ITRACONAZOLE THERAPY FOR INVASIVE ASPERGILLOSIS (VOL 97, PG 135, 1994).” American Journal of Medicine 97, no. 5 (November 1, 1994): 497–497.

Scholars@Duke

Casadevall, A., J. Mukherjee, R. Yuan, and J. Perfect. “Management of injuries caused by Cryptococcus neoformans--contaminated needles..” Clin Infect Dis 19, no. 5 (November 1994): 951–53. https://doi.org/10.1093/clinids/19.5.951.

PMID
7893887
Full Text

Cox, G. M., W. A. Schell, R. L. Scher, and J. R. Perfect. “First report of involvement of Nodulisporium species in human disease..” J Clin Microbiol 32, no. 9 (September 1994): 2301–4.

PMID
7814564
Scholars@Duke

Denning, D. W., J. Y. Lee, J. S. Hostetler, P. Pappas, C. A. Kauffman, D. H. Dewsnup, J. N. Galgiani, J. R. Graybill, A. M. Sugar, and A. Catanzaro. “NIAID Mycoses Study Group Multicenter Trial of Oral Itraconazole Therapy for Invasive Aspergillosis..” Am J Med 97, no. 2 (August 1994): 135–44. https://doi.org/10.1016/0002-9343(94)90023-x.

PMID
8059779
Full Text

Gilbert, C., B. Meisenberg, J. Vredenburgh, M. Ross, A. Hussein, J. Perfect, and W. P. Peters. “Sequential prophylactic oral and empiric once-daily parenteral antibiotics for neutropenia and fever after high-dose chemotherapy and autologous bone marrow support..” J Clin Oncol 12, no. 5 (May 1994): 1005–11. https://doi.org/10.1200/JCO.1994.12.5.1005.

PMID
8164024
Full Text

Denning, D. W., J. Y. Lee, J. S. Hostetler, P. Pappas, C. A. Kauffman, D. H. Dewsnup, J. N. Galgiani, et al. “Erratum: NIAID mycoses study group multicenter trial of oral itraconazole therapy for invasive aspergillosis (American Journal of Medicine (August 1994) 97 (135-144)).” American Journal of Medicine 97, no. 5 (January 1, 1994). https://doi.org/10.1016/0002-9343(94)90341-7.

Full Text

Perfect, J. R., and K. A. Wright. “Amphotericin B lipid complex in the treatment of experimental cryptococcal meningitis and disseminated candidosis..” J Antimicrob Chemother 33, no. 1 (January 1994): 73–81. https://doi.org/10.1093/jac/33.1.73.

PMID
8157577
Full Text

Lortholary, O., J. Robert, and J. Le Bras. “[Epidemiology of chemoresistant Plasmodium falciparum]..” Ann Med Interne (Paris) 145, no. 6 (1994): 429–32.

PMID
7864505
Scholars@Duke

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