John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Perfect, JR, Wong, B, Chang, YC, Kwon-Chung, KJ, and Williamson, PR. "Cryptococcus neoformans: Virulence and host defences." Medical Mycology, Supplement 36, no. 1 (1998): 79-86.

Scholars@Duke

Alspaugh, JA, Perfect, JR, and Heitman, J. "Cryptococcus neoformans mating and virulence are regulated by the G-protein alpha subunit GPA1 and cAMP." Genes Dev 11, no. 23 (December 1, 1997): 3206-3217.

PMID
9389652
Scholars@Duke

Alexander, BD, and Perfect, JR. "Antifungal resistance trends towards the year 2000. Implications for therapy and new approaches." Drugs 54, no. 5 (November 1997): 657-678. (Review)

PMID
9360056
Scholars@Duke

Toffaletti, DL, and Perfect, JR. "Study of Cryptococcus neoformans actin gene regulation with a beta-galactosidase-actin fusion." J Med Vet Mycol 35, no. 5 (September 1997): 313-320.

PMID
9402523
Scholars@Duke

Del Poeta, M, Schell, WA, and Perfect, JR. "In vitro antifungal activity of pneumocandin L-743,872 against a variety of clinically important molds." Antimicrob Agents Chemother 41, no. 8 (August 1997): 1835-1836.

PMID
9257774
Scholars@Duke

Odom, A, Muir, S, Lim, E, Toffaletti, DL, Perfect, J, and Heitman, J. "Calcineurin is required for virulence of Cryptococcus neoformans." The Embo Journal 16, no. 10 (May 1997): 2576-2589.

PMID
9184205
Full Text

Odom, A, Del Poeta, M, Perfect, J, and Heitman, J. "The immunosuppressant FK506 and its nonimmunosuppressive analog L-685,818 are toxic to Cryptococcus neoformans by inhibition of a common target protein." Antimicrob Agents Chemother 41, no. 1 (January 1997): 156-161.

PMID
8980772
Scholars@Duke

Pappas, P, Perfect, J, Henderson, H, Kauffman, C, Saccente, M, Haas, D, Pankey, G, Lancaster, D, Holloway, M, Cloud, G, and Dismukes, W. "Cryptococcosis in non-HIV infected patients: A multicemer survey." Clinical Infectious Diseases 25, no. 2 (1997): 379--.

Scholars@Duke

Alspaugh, JA, and Perfect, JR. "Infections due to zygomycetes and other rare fungal opportunists." Seminars in Respiratory and Critical Care Medicine 18, no. 3 (1997): 265-279.

Scholars@Duke

Alspaugh, JA, Perfect, JR, and Heitman, J. "GPA1 regulates capsule formation, melanin production, mating, and virulence in Cryptococcus neoformans." Clinical Infectious Diseases 25, no. 2 (1997): 356--.

Scholars@Duke

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