John Robert Perfect, MD

Professor of Medicine
James B. Duke Distinguished Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Kauffman, C. A., P. G. Pappas, D. S. McKinsey, R. A. Greenfield, J. R. Perfect, G. A. Cloud, C. J. Thomas, and W. E. Dismukes. “Treatment of lymphocutaneous and visceral sporotrichosis with fluconazole.” Clin Infect Dis 22, no. 1 (January 1996): 46–50. https://doi.org/10.1093/clinids/22.1.46.

PMID
8824965
Full Text

Morris, A., W. A. Schell, D. McDonagh, S. Chaffee, and J. R. Perfect. “Pneumonia due to Fonsecaea pedrosoi and cerebral abscesses due to Emericella nidulans in a bone marrow transplant recipient.” Clin Infect Dis 21, no. 5 (November 1995): 1346–48. https://doi.org/10.1093/clinids/21.5.1346.

PMID
8589181
Full Text

Mitchell, T. G., and J. R. Perfect. “Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans.” Clin Microbiol Rev 8, no. 4 (October 1995): 515–48.

PMID
8665468
Scholars@Duke

Pappas, P. G., C. A. Kauffman, J. Perfect, P. C. Johnson, D. S. McKinsey, D. M. Bamberger, R. Hamill, P. K. Sharkey, S. W. Chapman, and J. D. Sobel. “Alopecia associated with fluconazole therapy.” Ann Intern Med 123, no. 5 (September 1, 1995): 354–57. https://doi.org/10.7326/0003-4819-123-5-199509010-00006.

PMID
7625624
Full Text

Cox, G. M., T. H. Rude, C. C. Dykstra, and J. R. Perfect. “The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.” J Med Vet Mycol 33, no. 4 (July 1995): 261–66. https://doi.org/10.1080/02681219580000521.

PMID
8531025
Full Text

Berg, D., J. A. Garcia, W. A. Schell, J. R. Perfect, and J. C. Murray. “Cutaneous infection caused by Curvularia pallescens: a case report and review of the spectrum of disease.” J Am Acad Dermatol 32, no. 2 Pt 2 (February 1995): 375–78. https://doi.org/10.1016/0190-9622(95)90408-5.

PMID
7829744
Full Text

Cox, G. M., T. H. Rude, C. C. Dykstra, and J. R. Perfect. “The actin gene from cryptococcus neoformans: Structure and phylogenetic analysis.” Medical Mycology 33, no. 4 (January 1, 1995): 261–66. https://doi.org/10.1080/02681219580000521.

Full Text

Lodge, J. K., E. Jackson-Machelski, D. L. Toffaletti, J. R. Perfect, and J. I. Gordon. “Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans.” Proc Natl Acad Sci U S A 91, no. 25 (December 6, 1994): 12008–12. https://doi.org/10.1073/pnas.91.25.12008.

PMID
7991574
Full Text

DENNING, D. W., J. Y. LEE, J. S. HOSTETLER, P. PAPPAS, C. A. KAUFFMAN, D. H. DEWSNUP, J. N. GALGIANI, et al. “NIAID MYCOSES STUDY-GROUP MULTICENTER TRIAL OF ORAL ITRACONAZOLE THERAPY FOR INVASIVE ASPERGILLOSIS (VOL 97, PG 135, 1994).” American Journal of Medicine 97, no. 5 (November 1, 1994): 497–497.

Scholars@Duke

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