John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Perfect, J. R., K. A. Wright, M. M. Hobbs, and D. T. Durack. “Treatment of experimental cryptococcal meningitis and disseminated candidiasis with SCH39304..” Antimicrob Agents Chemother 33, no. 10 (October 1989): 1735–40. https://doi.org/10.1128/aac.33.10.1735.

PMID
2556078
Full Text

Perfect, J. R., M. M. Hobbs, K. A. Wright, and D. T. Durack. “Treatment of experimental disseminated candidiasis with cilofungin..” Antimicrob Agents Chemother 33, no. 10 (October 1989): 1811–12. https://doi.org/10.1128/aac.33.10.1811.

PMID
2589848
Full Text

Ingram, C. W., J. Sennesh, J. N. Cooper, and J. R. Perfect. “Disseminated zygomycosis: report of four cases and review..” Rev Infect Dis 11, no. 5 (September 1989): 741–54. https://doi.org/10.1093/clinids/11.5.741.

PMID
2682947
Full Text

Perfect, J. R., B. B. Magee, and P. T. Magee. “Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis..” Infect Immun 57, no. 9 (September 1989): 2624–27.

PMID
2668180
Scholars@Duke

Perfect, J. R. “Cryptococcosis..” Infect Dis Clin North Am 3, no. 1 (March 1989): 77–102.

PMID
2647836
Scholars@Duke

Sane, A., S. Manzi, J. Perfect, A. J. Herzberg, and J. O. Moore. “Deferoxamine treatment as a risk factor for zygomycete infection..” J Infect Dis 159, no. 1 (January 1989): 151–52. https://doi.org/10.1093/infdis/159.1.151.

PMID
2909637
Full Text

Kaplan, A. H., D. J. Weber, E. Z. Oddone, and J. R. Perfect. “Infection due to Actinobacillus actinomycetemcomitans: 15 cases and review..” Rev Infect Dis 11, no. 1 (January 1989): 46–63. https://doi.org/10.1093/clinids/11.1.46.

PMID
2644690
Full Text

Pages