John Robert Perfect, MD

Professor of Medicine
James B. Duke Distinguished Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan, Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., The University of Toledo, 1974


Perfect, J. R. “Cryptococcosis.” Infect Dis Clin North Am 3, no. 1 (March 1989): 77–102.


Kaplan, A. H., D. J. Weber, E. Z. Oddone, and J. R. Perfect. “Infection due to Actinobacillus actinomycetemcomitans: 15 cases and review.” Rev Infect Dis 11, no. 1 (January 1989): 46–63.

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Sane, A., S. Manzi, J. Perfect, A. J. Herzberg, and J. O. Moore. “Deferoxamine treatment as a risk factor for zygomycete infection.” J Infect Dis 159, no. 1 (January 1989): 151–52.

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Drew, R. H., J. R. Perfect, and H. A. Gallis. “Use of fluconazole in a patient with documented malabsorption of ketoconazole.” Clin Pharm 7, no. 8 (August 1988): 622–23.


Perfect, J. R., M. M. Hobbs, D. L. Granger, and D. T. Durack. “Cerebrospinal fluid macrophage response to experimental cryptococcal meningitis: relationship between in vivo and in vitro measurements of cytotoxicity.” Infect Immun 56, no. 4 (April 1988): 849–54.

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Granger, D. L., J. B. Hibbs, J. R. Perfect, and D. T. Durack. “Specific amino acid (L-arginine) requirement for the microbiostatic activity of murine macrophages.” J Clin Invest 81, no. 4 (April 1988): 1129–36.

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