John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

PERFECT, JR, and DURACK, DT. "THE ANTIFUNGAL ACTIVITY OF THE TRIANGLE UK-49,858 IN THE TREATMENT OF EXPERIMENTAL CANDIDA PYELONEPHRITIS AND CRYPTOCOCCAL MENINGITIS." July 1986.

Scholars@Duke

Perfect, JR, Savani, DV, and Durack, DT. "Comparison of itraconazole and fluconazole in treatment of cryptococcal meningitis and candida pyelonephritis in rabbits." Antimicrob Agents Chemother 29, no. 4 (April 1986): 579-583.

PMID
3010846
Scholars@Duke

GRANGER, DL, PERFECT, JR, and DURACK, DT. "ROLE OF IRON AND HEME BINDING SERUM-PROTEINS IN MACROPHAGE FUNGISTASIS." April 1986.

Scholars@Duke

PERFECT, JR, HOBBS, MM, GRANGER, DL, and DURACK, DT. "DYNAMICS OF CEREBROSPINAL-FLUID MACROPHAGE ACTIVATION IN EXPERIMENTAL CRYPTOCOCCAL MENINGITIS." April 1986.

Scholars@Duke

Granger, DL, Perfect, JR, and Durack, DT. "Macrophage-mediated fungistasis in vitro: requirements for intracellular and extracellular cytotoxicity." J Immunol 136, no. 2 (January 1986): 672-680.

PMID
3510251
Scholars@Duke

PERFECT, J. "Comparison of itraconazole and flucanazole in the treatment of cryptococcal meningitis and Candida pyelonephritis in rabbits." Antimicr Agents Chemoth 29 (1986): 579-583.

Scholars@Duke

Perfect, JR, and Durack, DT. "Comparison of amphotericin B and N-D-ornithyl amphotericin B methyl ester in experimental cryptococcal meningitis and Candida albicans endocarditis with pyelonephritis." Antimicrob Agents Chemother 28, no. 6 (December 1985): 751-755.

PMID
4083860
Scholars@Duke

Perfect, JR, and Durack, DT. "Effects of cyclosporine in experimental cryptococcal meningitis." Infect Immun 50, no. 1 (October 1985): 22-26.

PMID
3899932
Scholars@Duke

Granger, DL, Perfect, JR, and Durack, DT. "Virulence of Cryptococcus neoformans. Regulation of capsule synthesis by carbon dioxide." The Journal of Clinical Investigation 76, no. 2 (August 1985): 508-516.

PMID
3928681
Full Text

Perfect, JR, and Durack, DT. "Penetration of imidazoles and triazoles into cerebrospinal fluid of rabbits." J Antimicrob Chemother 16, no. 1 (July 1985): 81-86.

PMID
2995303
Scholars@Duke

Pages