John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Granger, D. L., J. R. Perfect, and D. T. Durack. “Virulence of Cryptococcus neoformans. Regulation of capsule synthesis by carbon dioxide..” J Clin Invest 76, no. 2 (August 1985): 508–16. https://doi.org/10.1172/JCI112000.

PMID
3928681
Full Text

Perfect, J. R., and D. T. Durack. “Penetration of imidazoles and triazoles into cerebrospinal fluid of rabbits..” J Antimicrob Chemother 16, no. 1 (July 1985): 81–86. https://doi.org/10.1093/jac/16.1.81.

PMID
2995303
Full Text

Perfect, J. R., and B. A. Seaworth. “Penile cryptococcosis with review of mycotic infections of penis..” Urology 25, no. 5 (May 1985): 528–31. https://doi.org/10.1016/0090-4295(85)90469-8.

PMID
3992781
Full Text

Perfect, J. R., and D. T. Durack. “Chemotactic activity of cerebrospinal fluid in experimental cryptococcal meningitis..” Sabouraudia 23, no. 1 (February 1985): 37–45.

PMID
3992427
Scholars@Duke

LALLINGER, G. J., S. L. REINER, J. R. PERFECT, D. L. GRANGER, and D. T. DURACK. “SERUM THERAPY OF EXPERIMENTAL NAEGLERIA FOWLERI MENINGITIS.” In Clinical Research, 33:A408–A408. SLACK INC, 1985.

Scholars@Duke

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “SERUM FACTOR(S) WHICH PROMOTES MACROPHAGE FUNGISTATIC ACTION.” In Journal of Leukocyte Biology, 38:133–133. FEDERATION AMER SOC EXP BIOL, 1985.

Scholars@Duke

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “MACROPHAGES REQUIRE A SERUM FACTOR(S) FOR EFFICIENT FUNGISTASIS.” In Clinical Research, 33:A565–A565. SLACK INC, 1985.

Scholars@Duke

Smego, R. A., J. R. Perfect, and D. T. Durack. “Combined therapy with amphotericin B and 5-fluorocytosine for Candida meningitis..” Rev Infect Dis 6, no. 6 (November 1984): 791–801.

PMID
6522917
Scholars@Duke

Smego, R. A., P. W. Devoe, H. A. Sampson, J. R. Perfect, C. M. Wilfert, and R. H. Buckley. “Candida meningitis in two children with severe combined immunodeficiency..” J Pediatr 104, no. 6 (June 1984): 902–4. https://doi.org/10.1016/s0022-3476(84)80493-x.

PMID
6726524
Full Text

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