John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Perfect, JR, and Seaworth, BA. "Penile cryptococcosis with review of mycotic infections of penis." Urology 25, no. 5 (May 1985): 528-531.

PMID
3992781
Scholars@Duke

Perfect, JR, and Durack, DT. "Chemotactic activity of cerebrospinal fluid in experimental cryptococcal meningitis." Sabouraudia 23, no. 1 (February 1985): 37-45.

PMID
3992427
Scholars@Duke

Perfect, JR, and Durack, DT. "Chemotactic activity of cerebrospinal fluid in experimental cryptococcal meningitis." Medical Mycology 23, no. 1 (January 1, 1985): 37-45.

Full Text

LALLINGER, GJ, REINER, SL, PERFECT, JR, GRANGER, DL, and DURACK, DT. "SERUM THERAPY OF EXPERIMENTAL NAEGLERIA FOWLERI MENINGITIS." 1985.

Scholars@Duke

GRANGER, DL, PERFECT, JR, and DURACK, DT. "SERUM FACTOR(S) WHICH PROMOTES MACROPHAGE FUNGISTATIC ACTION." 1985.

Scholars@Duke

GRANGER, DL, PERFECT, JR, and DURACK, DT. "MACROPHAGES REQUIRE A SERUM FACTOR(S) FOR EFFICIENT FUNGISTASIS." 1985.

Scholars@Duke

Smego, RA, Perfect, JR, and Durack, DT. "Combined therapy with amphotericin B and 5-fluorocytosine for Candida meningitis." Reviews of Infectious Diseases 6, no. 6 (November 1984): 791-801.

PMID
6522917
Scholars@Duke

Smego, RA, Devoe, PW, Sampson, HA, Perfect, JR, Wilfert, CM, and Buckley, RH. "Candida meningitis in two children with severe combined immunodeficiency." J Pediatr 104, no. 6 (June 1984): 902-904.

PMID
6726524
Scholars@Duke

GRANGER, DL, PERFECT, JR, and DURACK, DT. "CARBON-DIOXIDE REGULATES CAPSULE PRODUCTION BY CRYPTOCOCCUS-NEOFORMANS." 1984.

Scholars@Duke

PERFECT, JR, GRANGER, DL, and DURACK, DT. "CYCLOSPORINE PREDISPOSES TO EXPERIMENTAL CRYPTOCOCCAL MENINGITIS." 1984.

Scholars@Duke

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