John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Granger, D. L., J. R. Perfect, and D. T. Durack. “Macrophage-mediated fungistasis in vitro: requirements for intracellular and extracellular cytotoxicity..” J Immunol 136, no. 2 (January 1986): 672–80.

PMID
3510251
Scholars@Duke

PERFECT, J. R. “Comparison of itraconazole and flucanazole in the treatment of cryptococcal meningitis and Candida pyelonephritis in rabbits.” Antimicr Agents Chemoth 29 (1986): 579–83.

Scholars@Duke

Perfect, J. R., and D. T. Durack. “Comparison of amphotericin B and N-D-ornithyl amphotericin B methyl ester in experimental cryptococcal meningitis and Candida albicans endocarditis with pyelonephritis..” Antimicrob Agents Chemother 28, no. 6 (December 1985): 751–55. https://doi.org/10.1128/aac.28.6.751.

PMID
4083860
Full Text

Perfect, J. R., and D. T. Durack. “Effects of cyclosporine in experimental cryptococcal meningitis..” Infect Immun 50, no. 1 (October 1985): 22–26.

PMID
3899932
Scholars@Duke

Granger, D. L., J. R. Perfect, and D. T. Durack. “Virulence of Cryptococcus neoformans. Regulation of capsule synthesis by carbon dioxide..” J Clin Invest 76, no. 2 (August 1985): 508–16. https://doi.org/10.1172/JCI112000.

PMID
3928681
Full Text

Perfect, J. R., and D. T. Durack. “Penetration of imidazoles and triazoles into cerebrospinal fluid of rabbits..” J Antimicrob Chemother 16, no. 1 (July 1985): 81–86. https://doi.org/10.1093/jac/16.1.81.

PMID
2995303
Full Text

Perfect, J. R., and B. A. Seaworth. “Penile cryptococcosis with review of mycotic infections of penis..” Urology 25, no. 5 (May 1985): 528–31.

PMID
3992781
Scholars@Duke

Perfect, J. R., and D. T. Durack. “Chemotactic activity of cerebrospinal fluid in experimental cryptococcal meningitis..” Sabouraudia 23, no. 1 (February 1985): 37–45.

PMID
3992427
Scholars@Duke

GRANGER, D. L., J. R. PERFECT, and D. T. DURACK. “MACROPHAGES REQUIRE A SERUM FACTOR(S) FOR EFFICIENT FUNGISTASIS.” In Clinical Research, 33:A565–A565. SLACK INC, 1985.

Scholars@Duke

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