John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974


Mourad, A, Johnson, M, and Perfect, J. "A Decade of Voriconazole Use at Duke University Medical Center: What are the Challenges with Dosing?." New Orleans, LA,: American Society of Microbiology - Microbe, June 4, 2017.


Fraser, JA, Giles, SS, Wenink, EC, Geunes-Boyer, SG, Wright, JR, Diezmann, S, Allen, A, Stajich, JE, Dietrich, FS, Perfect, JR, and Heitman, J. "Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak." Nature 437, no. 7063 (October 9, 2005): 1360-1364.

Full Text

Drew, RH, Perfect, JR, Srinath, L, Kurkimilis, E, Dowzicky, M, and Talbot, GH. "Treatment of methicillin-resistant Staphylococcus aureus infections with quinupristin-dalfopristin in patients intolerant of or failing prior therapy." Journal of Antimicrobial Chemotherapy 46, no. 5 (November 29, 2000): 775-784.