John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Perfect, J. R. “Mediastinitis complicating cellulitis of the head and neck..” Southern Medical Journal 73, no. 10 (October 1980): 1378–80.

PMID
7434054
Scholars@Duke

Perfect, J. R., S. D. Lang, and D. T. Durack. “Chronic cryptococcal meningitis: a new experimental model in rabbits..” The American Journal of Pathology 101, no. 1 (October 1980): 177–94.

PMID
7004196
Scholars@Duke

PERFECT, J. R., S. D. R. LANG, and D. T. DURACK. “INFLUENCE OF HUMORAL IMMUNITY ON CRYPTOCOCCAL MENINGITIS IN RABBITS.” In Clinical Research, 28:A376–A376. SLACK INC, 1980.

Scholars@Duke

Perfect, J. R., S. D. Lang, and D. T. Durack. “Comparison of cotrimoxazole, ampicillin, and chloramphenicol in treatment of experimental Haemophilus influenzae type B meningitis..” Antimicrobial Agents and Chemotherapy 17, no. 1 (January 1980): 43–48. https://doi.org/10.1128/aac.17.1.43.

PMID
6965442
Full Text

Perfect, J. R., S. D. R. Lang, and D. T. Durack. “Comparison of cotrimoxazole (TMP/SMX), ampicillin (AMP) and chloramphenicol (CHLORO) in treatment of experimental Haemophilus influenzae type B (HITB) meningitis.” Clinical Research 27, no. 2 (January 1, 1979).

Scholars@Duke

Mourad, A., Melissa Johnson, and John Perfect. “A Decade of Voriconazole Use at Duke University Medical Center: What are the Challenges with Dosing?.” New Orleans, LA: American Society of Microbiology - Microbe, 2017.

Scholars@Duke

Fraser, James A., Steven S. Giles, Emily C. Wenink, Scarlett G. Geunes-Boyer, Jo Rae Wright, Stephanie Diezmann, Andria Allen, et al. “Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak..” Nature 437, no. 7063 (October 9, 2005): 1360–64. https://doi.org/10.1038/nature04220.

PMID
16222245
Full Text

Drew, R. H., J. R. Perfect, L. Srinath, E. Kurkimilis, M. Dowzicky, and G. H. Talbot. “Treatment of methicillin-resistant Staphylococcus aureus infections with quinupristin-dalfopristin in patients intolerant of or failing prior therapy.” Journal of Antimicrobial Chemotherapy 46, no. 5 (November 29, 2000): 775–84.

Scholars@Duke

Pages