John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Duarte, R. F., J. López-Jiménez, O. A. Comely, M. Laverdiere, D. Helfgott, S. Haider, P. Chandrasekar, et al. “Phase 1b study of new posaconazole tablet for prevention of invasive fungal infections in high-risk patients with neutropenia.” Antimicrobial Agents and Chemotherapy 58, no. 10 (October 1, 2014): 5758–65. https://doi.org/10.1128/AAC03050-14.

Full Text

Duarte, Rafael F., Javier López-Jiménez, Oliver A. Cornely, Michel Laverdiere, David Helfgott, Shariq Haider, Pranatharthi Chandrasekar, et al. “Phase 1b study of new posaconazole tablet for prevention of invasive fungal infections in high-risk patients with neutropenia..” Antimicrob Agents Chemother 58, no. 10 (October 2014): 5758–65. https://doi.org/10.1128/AAC.03050-14.

PMID
25049247
Full Text

Kumar, Vinod, Shih-Chin Cheng, Melissa D. Johnson, Sanne P. Smeekens, Agnieszka Wojtowicz, Evangelos Giamarellos-Bourboulis, Juha Karjalainen, et al. “Immunochip SNP array identifies novel genetic variants conferring susceptibility to candidaemia..” Nat Commun 5 (September 8, 2014). https://doi.org/10.1038/ncomms5675.

PMID
25197941
Full Text

Janout, Vaclav, Celine Bienvenu, Wiley Schell, John R. Perfect, and Steven L. Regen. “Molecular umbrella-amphotericin B conjugates..” Bioconjug Chem 25, no. 8 (August 20, 2014): 1408–11. https://doi.org/10.1021/bc500277v.

PMID
25068916
Full Text

Jarvis, Joseph N., Tihana Bicanic, Angela Loyse, Graeme Meintjes, Louise Hogan, Chrissy H. Roberts, Shmuel Shoham, John R. Perfect, Nelesh P. Govender, and Thomas S. Harrison. “Very low levels of 25-hydroxyvitamin D are not associated with immunologic changes or clinical outcome in South African patients with HIV-associated cryptococcal meningitis..” Clin Infect Dis 59, no. 4 (August 15, 2014): 493–500. https://doi.org/10.1093/cid/ciu349.

PMID
24825871
Full Text

Paul DiMondi, V., Mary L. Townsend, Melissa Johnson, and Michael Durkin. “Antifungal catheter lock therapy for the management of a persistent Candida albicans bloodstream infection in an adult receiving hemodialysis..” Pharmacotherapy 34, no. 7 (July 2014): e120–27. https://doi.org/10.1002/phar.1433.

PMID
24782326
Full Text

Kung, Hsiang-Chi, Melissa D. Johnson, Richard H. Drew, Paramita Saha-Chaudhuri, and John R. Perfect. “Clinical effectiveness of posaconazole versus fluconazole as antifungal prophylaxis in hematology-oncology patients: a retrospective cohort study..” Cancer Med 3, no. 3 (June 2014): 667–73. https://doi.org/10.1002/cam4.225.

PMID
24644249
Full Text

Pages