Jonathan Allen Cohn, MD

Professor of Medicine
Associate Professor of Cell Biology
Professor in Pediatrics
Campus mail DUMC Box 3256, Durham, NC 27710
Phone (919) 684-6879
Email address jonathan.cohn@duke.edu

In many epithelial tissues, the protein mainly responsible for controlling transepithelial fluid movement is the the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR was originally identified as the protein product of the gene causing cystic fibrosis (CF). CFTR functions as a chloride channel regulated by protein kinase A (PKA). This laboratory is studying the role and regulation of CFTR. One project focuses on how PKA acts on CFTR. In this project, recombinant peptide models are being used to model the cytoplasmic domains of CFTR responsible for activating the protein's chloride's chloride channel function. Site-directed mutagenesis is being used to produce modified peptides to study how different individual serine phosphoryation contribute to CFTR regulation. A second project concerns DF508, the most common mutation among patients with CF. This mutation affects CFTR function by preventing normal folding and intracellular trafficking of the newly synthesized mutant protein. This project is examining the mislocalization of DF508-CFTR in tissues and cell lines with the long term goal of developing strategies to prevent the mutant protein from mislocalizing. A third project concerns the role of CFTR in chronic pancreatitic diseases. Emerging data about the role of CFTR during normal pancreatic secretion suggests that dysfunction of this protein may lead to pancreatic diseases such as chronic pancreatitis and pancreatic cancer. Patients with these chronic pancreatic diseases are being tested for CFTR mutations and for evidence of defective CFTR function.

Education and Training

  • Fellow in Gastroenterology, Medicine, Yale University, 1981 - 1984
  • Medical Resident, Medicine, University of California San Francisco, School of Medicine, 1980 - 1981
  • Medical Resident, Medicine, University of Alabama Birmingham, 1978 - 1980
  • M.D., Cornell University, 1978

Publications

Tietz, Pamela S., Raul A. Marinelli, Xian-Ming Chen, Bing Huang, Jonathan Cohn, Jolanta Kole, Mark A. McNiven, Seth Alper, and Nicholas F. LaRusso. “Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.” J Biol Chem 278, no. 22 (May 30, 2003): 20413–19. https://doi.org/10.1074/jbc.M302108200.

PMID
12660234
Full Text

Mansfield, S. G., R. Hawkins-Clark, J. A. Cohn, J. Kole, L. G. Mitchell, and M. A. Garcia-Blanco. “5 ' exon replacement by spliceosome mediated RNA trans-splicing.” In Molecular Therapy, 7:S301–2. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2003.

Scholars@Duke

Cohn, Jonathan A. “Motion--genetic testing is useful in the diagnosis of nonhereditary pancreatic conditions: arguments against the motion.” Can J Gastroenterol 17, no. 1 (January 2003): 53–55. https://doi.org/10.1155/2003/169280.

PMID
12560856
Full Text

Feng, J., J. A. Cohn, J. P. Neoptolemus, Z. Jiang, W. Greenhalf, I. Ellis, and S. S. Sommer. “Comprehensive detection of CFTR mutations in idiopathic chronic pancreatitis.” In American Journal of Human Genetics, 71:483–483. UNIV CHICAGO PRESS, 2002.

Scholars@Duke

Cohn, Jonathan A., Peadar G. Noone, and Paul S. Jowell. “Idiopathic pancreatitis related to CFTR: complex inheritance and identification of a modifier gene.” J Investig Med 50, no. 5 (September 2002): 247S-255S. https://doi.org/10.1136/jim-50-suppl5-01.

PMID
12227654
Full Text

Liu, Xiaoming, Qinshi Jiang, S Gary Mansfield, M. Puttaraju, Yulong Zhang, Weihong Zhou, Jonathan A. Cohn, Mariano A. Garcia-Blanco, Lloyd G. Mitchell, and John F. Engelhardt. “Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing.” Nat Biotechnol 20, no. 1 (January 2002): 47–52. https://doi.org/10.1038/nbt0102-47.

PMID
11753361
Full Text

Noone, P. G., Z. Zhou, L. M. Silverman, P. S. Jowell, M. R. Knowles, and J. A. Cohn. “Cystic fibrosis gene mutations and pancreatitis risk: relation to epithelial ion transport and trypsin inhibitor gene mutations.” Gastroenterology 121, no. 6 (December 2001): 1310–19. https://doi.org/10.1053/gast.2001.29673.

PMID
11729110
Full Text

Dérand, R., L. Bulteau-Pignoux, Y. Mettey, O. Zegarra-Moran, L. D. Howell, C. Randak, L. J. Galietta, et al. “Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation.” Am J Physiol Cell Physiol 281, no. 5 (November 2001): C1657–66. https://doi.org/10.1152/ajpcell.2001.281.5.C1657.

PMID
11600430
Full Text

Pages