Jonathan Allen Cohn, MD

Professor of Medicine
Associate Professor of Cell Biology
Professor in Pediatrics
Campus mail DUMC Box 3256, Durham, NC 27710
Phone (919) 684-6879
Email address jonathan.cohn@duke.edu

In many epithelial tissues, the protein mainly responsible for controlling transepithelial fluid movement is the the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR was originally identified as the protein product of the gene causing cystic fibrosis (CF). CFTR functions as a chloride channel regulated by protein kinase A (PKA). This laboratory is studying the role and regulation of CFTR. One project focuses on how PKA acts on CFTR. In this project, recombinant peptide models are being used to model the cytoplasmic domains of CFTR responsible for activating the protein's chloride's chloride channel function. Site-directed mutagenesis is being used to produce modified peptides to study how different individual serine phosphoryation contribute to CFTR regulation. A second project concerns DF508, the most common mutation among patients with CF. This mutation affects CFTR function by preventing normal folding and intracellular trafficking of the newly synthesized mutant protein. This project is examining the mislocalization of DF508-CFTR in tissues and cell lines with the long term goal of developing strategies to prevent the mutant protein from mislocalizing. A third project concerns the role of CFTR in chronic pancreatitic diseases. Emerging data about the role of CFTR during normal pancreatic secretion suggests that dysfunction of this protein may lead to pancreatic diseases such as chronic pancreatitis and pancreatic cancer. Patients with these chronic pancreatic diseases are being tested for CFTR mutations and for evidence of defective CFTR function.

Education and Training

  • Fellow in Gastroenterology, Medicine, Yale University, 1981 - 1984
  • Medical Resident, Medicine, University of California San Francisco, School of Medicine, 1980 - 1981
  • Medical Resident, Medicine, University of Alabama Birmingham, 1978 - 1980
  • M.D., Cornell University, 1978

Publications

Cohn, J. A., K. J. Friedman, P. G. Noone, M. R. Knowles, L. M. Silverman, and P. S. Jowell. “Relation between mutations of the cystic fibrosis gene and idiopathic pancreatitis.” N Engl J Med 339, no. 10 (September 3, 1998): 653–58. https://doi.org/10.1056/NEJM199809033391002.

PMID
9725922
Full Text

Howell, L. D., L. Chien, and J. A. Cohn. “Protein kinase a regulates ATP hydrolysis by the CFTR NBD1/R domain.” Gastroenterology 114 (April 1998): A379–A379. https://doi.org/10.1016/s0016-5085(98)81536-9.

Full Text

Cohn, J. A., K. J. Friedman, L. M. Silverman, P. G. Noone, C. Q. Lee, M. R. Knowles, and P. S. Jowell. “Abnormalities of the CFTR gene predispose to idiopathic chronic pancreatitis.” Gastroenterology 114 (April 1998): A449–A449. https://doi.org/10.1016/s0016-5085(98)81815-5.

Full Text

Xiong, X., A. Bragin, J. H. Widdicombe, J. Cohn, and W. R. Skach. “Structural cues involved in endoplasmic reticulum degradation of G85E and G91R mutant cystic fibrosis transmembrane conductance regulator.” J Clin Invest 100, no. 5 (September 1, 1997): 1079–88. https://doi.org/10.1172/JCI119618.

PMID
9276724
Full Text

Abdullah, L. H., J. D. Conway, J. A. Cohn, and C. W. Davis. “Protein kinase C and Ca2+ activation of mucin secretion in airway goblet cells.” American Journal of Physiology Lung Cellular and Molecular Physiology 273, no. 1 (July 1, 1997): L201–10.

Scholars@Duke

Cohn, J. A., K. J. Friedman, L. M. Silverman, P. G. Noone, M. R. Knowles, and P. S. Jowell. “CFTR mutations predispose to chronic pancreatitis without cystic fibrosis lung disease.” In Gastroenterology, 112:A434–A434. W B SAUNDERS CO-ELSEVIER INC, 1997.

Scholars@Duke

Cohn, J. A., R. A. Borchardt, A. Y. R. Yu, and J. Kole. “Regulation of the nbd1/R domain of CFTR by ATP and by protein kinases.” In Gastroenterology, 112:A355–A355. W B SAUNDERS CO-ELSEVIER INC, 1997.

Scholars@Duke

Montrose-Rafizadeh, C., J. Kole, L. M. Bartkowski, L. H. Lee, D. L. Blackmon, S. E. Behnken, J. D. Gearhart, J. A. Cohn, and M. H. Montrose. “Gene targeting of a CFTR allele in HT29 human epithelial cells.” J Cell Physiol 170, no. 3 (March 1997): 299–308. https://doi.org/10.1002/(SICI)1097-4652(199703)170:3<299::AID-JCP11>3.0.CO;2-A.

PMID
9066787
Full Text

Torres, R. J., G. A. Altenberg, J. Cohn, and L. Reuss. “Polarized apical-membrane expression of cAMP-actlvated chloride channels in isolated epithelial cells.” Faseb Journal 10, no. 3 (December 1, 1996).

Scholars@Duke

Pages