Jonathan Allen Cohn, MD

Professor of Medicine
Associate Professor of Cell Biology
Professor in Pediatrics
Campus mail DUMC Box 3256, Durham, NC 27710
Phone (919) 684-6879
Email address jonathan.cohn@duke.edu

In many epithelial tissues, the protein mainly responsible for controlling transepithelial fluid movement is the the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR was originally identified as the protein product of the gene causing cystic fibrosis (CF). CFTR functions as a chloride channel regulated by protein kinase A (PKA). This laboratory is studying the role and regulation of CFTR. One project focuses on how PKA acts on CFTR. In this project, recombinant peptide models are being used to model the cytoplasmic domains of CFTR responsible for activating the protein's chloride's chloride channel function. Site-directed mutagenesis is being used to produce modified peptides to study how different individual serine phosphoryation contribute to CFTR regulation. A second project concerns DF508, the most common mutation among patients with CF. This mutation affects CFTR function by preventing normal folding and intracellular trafficking of the newly synthesized mutant protein. This project is examining the mislocalization of DF508-CFTR in tissues and cell lines with the long term goal of developing strategies to prevent the mutant protein from mislocalizing. A third project concerns the role of CFTR in chronic pancreatitic diseases. Emerging data about the role of CFTR during normal pancreatic secretion suggests that dysfunction of this protein may lead to pancreatic diseases such as chronic pancreatitis and pancreatic cancer. Patients with these chronic pancreatic diseases are being tested for CFTR mutations and for evidence of defective CFTR function.

Education and Training

  • Fellow in Gastroenterology, Medicine, Yale University, 1981 - 1984
  • Medical Resident, Medicine, University of California San Francisco, School of Medicine, 1980 - 1981
  • Medical Resident, Medicine, University of Alabama Birmingham, 1978 - 1980
  • M.D., Cornell University, 1978

Publications

Strong, T. V., D. J. Wilkinson, M. K. Mansoura, D. C. Devor, K. Henze, Y. Yang, J. M. Wilson, J. A. Cohn, D. C. Dawson, and R. A. Frizzell. “Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance.” Hum Mol Genet 2, no. 3 (March 1993): 225–30. https://doi.org/10.1093/hmg/2.3.225.

PMID
7684641
Full Text

BRADBURY, N. A., J. A. COHN, C. J. VENGLARIK, and R. J. BRIDGES. “THE CYSTIC-FIBROSIS GENE-PRODUCT (CFTR) IS IN ENDOCYTIC CLATHRIN COATED VESICLES.” In Faseb Journal, 7:A355–A355. FEDERATION AMER SOC EXP BIOL, 1993.

Scholars@Duke

Fitz, J. G., S. Basavappa, J. McGill, O. Melhus, and J. A. Cohn. “Regulation of membrane chloride currents in rat bile duct epithelial cells.” J Clin Invest 91, no. 1 (January 1993): 319–28. https://doi.org/10.1172/JCI116188.

PMID
7678606
Full Text

Basavappa, S., J. Middleton, A. W. Mangel, J. M. Mcgill, J. A. Cohn, and J. G. Fitz. “Cl- and K+ transport in human biliary cell lines.” Gastroenterology 104, no. 6 (1993): 1796–1805.

Scholars@Duke

Engelhardt, J. F., J. R. Yankaskas, S. A. Ernst, Y. Yang, C. R. Marino, R. C. Boucher, J. A. Cohn, and J. M. Wilson. “Submucosal glands are the predominant site of CFTR expression in the human bronchus.” Nat Genet 2, no. 3 (November 1992): 240–48. https://doi.org/10.1038/ng1192-240.

PMID
1285365
Full Text

Whitsett, J. A., C. R. Dey, B. R. Stripp, K. A. Wikenheiser, J. C. Clark, S. E. Wert, R. J. Gregory, A. E. Smith, J. A. Cohn, and J. M. Wilson. “Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.” Nat Genet 2, no. 1 (September 1992): 13–20. https://doi.org/10.1038/ng0992-13.

PMID
1284640
Full Text

Nguyen, T. D., G. G. Heintz, and J. A. Cohn. “Pituitary adenylate cyclase-activating polypeptide stimulates secretion in T84 cells.” Gastroenterology 103, no. 2 (August 1992): 539–44. https://doi.org/10.1016/0016-5085(92)90844-o.

PMID
1321772
Full Text

Picciotto, M. R., J. A. Cohn, G. Bertuzzi, P. Greengard, and A. C. Nairn. “Phosphorylation of the cystic fibrosis transmembrane conductance regulator.” J Biol Chem 267, no. 18 (June 25, 1992): 12742–52.

PMID
1377674
Scholars@Duke

FITZ, J. G., J. MCGILL, S. BASAVAPPA, and J. A. COHN. “BILE-DUCT EPITHELIAL-CELLS CONTAIN REGULATED CHLORIDE CHANNELS AND CFTR.” In Clinical Research, 40:A319–A319. SLACK INC, 1992.

Scholars@Duke

Kwatra, M. M., D. D. Bigner, and J. A. Cohn. “The ligand binding domain of the epidermal growth factor receptor is not required for receptor dimerization.” Biochim Biophys Acta 1134, no. 2 (March 16, 1992): 178–81. https://doi.org/10.1016/0167-4889(92)90042-a.

PMID
1554751
Full Text

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