Jonathan Allen Cohn, MD

Professor of Medicine
Associate Professor of Cell Biology
Professor in Pediatrics
Campus mail DUMC Box 3256, Durham, NC 27710
Phone (919) 684-6879
Email address jonathan.cohn@duke.edu

In many epithelial tissues, the protein mainly responsible for controlling transepithelial fluid movement is the the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR was originally identified as the protein product of the gene causing cystic fibrosis (CF). CFTR functions as a chloride channel regulated by protein kinase A (PKA). This laboratory is studying the role and regulation of CFTR. One project focuses on how PKA acts on CFTR. In this project, recombinant peptide models are being used to model the cytoplasmic domains of CFTR responsible for activating the protein's chloride's chloride channel function. Site-directed mutagenesis is being used to produce modified peptides to study how different individual serine phosphoryation contribute to CFTR regulation. A second project concerns DF508, the most common mutation among patients with CF. This mutation affects CFTR function by preventing normal folding and intracellular trafficking of the newly synthesized mutant protein. This project is examining the mislocalization of DF508-CFTR in tissues and cell lines with the long term goal of developing strategies to prevent the mutant protein from mislocalizing. A third project concerns the role of CFTR in chronic pancreatitic diseases. Emerging data about the role of CFTR during normal pancreatic secretion suggests that dysfunction of this protein may lead to pancreatic diseases such as chronic pancreatitis and pancreatic cancer. Patients with these chronic pancreatic diseases are being tested for CFTR mutations and for evidence of defective CFTR function.

Education and Training

  • Fellow in Gastroenterology, Medicine, Yale University, 1981 - 1984
  • Medical Resident, Medicine, University of California San Francisco, School of Medicine, 1980 - 1981
  • Medical Resident, Medicine, University of Alabama Birmingham, 1978 - 1980
  • M.D., Cornell University, 1978

Publications

Cohn, J. A., A. C. Nairn, C. R. Marino, O. Melhus, and J. Kole. “Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line.” Proc Natl Acad Sci U S A 89, no. 6 (March 15, 1992): 2340–44. https://doi.org/10.1073/pnas.89.6.2340.

PMID
1372442
Full Text

Cohn, J. A., O. Melhus, L. J. Page, K. L. Dittrich, and S. R. Vigna. “CFTR: development of high- affinity antibodies and localization in sweat gland.” Biochem Biophys Res Commun 181, no. 1 (November 27, 1991): 36–43. https://doi.org/10.1016/s0006-291x(05)81378-6.

PMID
1720311
Full Text

Marino, C. R., L. M. Matovcik, F. S. Gorelick, and J. A. Cohn. “Localization of the cystic fibrosis transmembrane conductance regulator in pancreas.” J Clin Invest 88, no. 2 (August 1991): 712–16. https://doi.org/10.1172/JCI115358.

PMID
1713921
Full Text

Nguyen, T. D., A. T. Canada, G. G. Heintz, T. W. Gettys, and J. A. Cohn. “Stimulation of secretion by the T84 colonic epithelial cell line with dietary flavonols.” Biochem Pharmacol 41, no. 12 (June 15, 1991): 1879–86. https://doi.org/10.1016/0006-2952(91)90127-q.

PMID
1645552
Full Text

COHN, J. A. “CAMP-DEPENDENT C1- TRANSPORT REQUIRES THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR IN T84 CELLS.” In Clinical Research, 39:A301–A301. SLACK INC, 1991.

Scholars@Duke

INGBAR, D. H., J. A. COHN, E. JACOBSON, and C. R. MARINO. “DEVELOPMENTAL REGULATION OF CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR CONTENT IN RAT LUNG.” In Clinical Research, 39:A149–A149. SLACK INC, 1991.

Scholars@Duke

Schwartz, R. D., G. Heuschneider, P. P. Edgar, and J. A. Cohn. “cAMP analogs inhibit gamma-aminobutyric acid-gated chloride flux and activate protein kinase A in brain synaptoneurosomes.” Mol Pharmacol 39, no. 3 (March 1991): 370–75.

PMID
1848658
Scholars@Duke

Marino, C. R., L. M. Matovcik, F. S. Gorelick, and J. A. Cohn. “Erratum: (The Journal of Clinical Investigation, Volume 88, No. 2, August 1991).” Journal of Clinical Investigation 88, no. 4 (January 1, 1991): 1433.

Scholars@Duke

BARRETT, K. E., J. A. COHN, P. A. HUOTT, S. I. WASSERMAN, and K. DHARMSATHAPHORN. “IMMUNE-RELATED INTESTINAL CHLORIDE SECRETION .2. EFFECT OF ADENOSINE ON T84 CELL-LINE.” American Journal of Physiology 258, no. 5 (May 1, 1990): C902–12.

Scholars@Duke

Barrett, K. E., J. A. Cohn, P. A. Huott, S. I. Wasserman, and K. Dharmsathaphorn. “Immune-related intestinal chloride secretion. II. Effect of adenosine on T84 cell line.” Am J Physiol 258, no. 5 Pt 1 (May 1990): C902–12. https://doi.org/10.1152/ajpcell.1990.258.5.C902.

PMID
2159233
Full Text

Pages