Michael Dee Gunn, MD

Professor of Medicine
Professor in Immunology
Associate Professor in Pathology
Member of the Duke Cancer Institute
Campus mail CARL Building Room 180B, Durham, NC 27703
Phone (919) 681-0840
Email address michael.gunn@duke.edu

The focus of my work is on understanding how dendritic cells, monocytes, and macrophages regulate immune responses, contribute to specific disease pathologies, and can be manipulated to stimulate or inhibit specific immune responses. We are also using our knowledge of immunology to develop diagnostics and therapeutics for a variety of human diseases. 

Lab History 

The lab started with our discovery of the lymphoid chemokines, which regulate the migration of lymphocytes and dendritic cells to and within secondary lymphoid organs.  We identified the chemokine (CCL21) that mediates the entry of naïve T cells and activated dendritic cells into lymph nodes and the chemokine (CXCL13) that mediates the entry of B cells into lymphoid follicles.  Our focus then shifted to understanding how specific cell types, primarily dendritic cells, and cell migration events regulate immune responses.  We identified murine plasmacytoid dendritic cells, the cell type that causes pulmonary immune pathology during influenza infection, the dendritic cell type that stimulates Th1 immune responses, and the cell type that induces neuronal injury in Alzheimer's disease.  Our current work continues these basic studies while applying our findings to models of human disease. 

Current Research 

Identification and characterization of inflammatory cell populations in models of human disease – We have developed advanced methods of flow cytometric analysis that allows us to quantify and fully characterize all inflammatory cell types in murine and human tissues.  Using these methods, we are working to identify the cells that mediate a variety of immune pathologies.  Examples include the identification of immune-stimulatory and immune-suppressive cell types in brain tumors, identification of the cells that induce vascular changes pulmonary hypertension, and characterization of the inflammatory response to a variety of infectious pathogens.

Tumor immune therapeutics – We have developed a novel cellular vaccine strategy for the treatment of cancer.  This strategy is much simpler, more cost effective, more clinically feasible, and much more efficacious than classic dendritic cell vaccines.  We are now testing this vaccine in various preclinical tumor models including melanoma and glioblastoma and will soon be advancing it to initial human clinical trials.

Treatment of Acute Lung Injury – We have identified the first small molecule pharmacologic agent that is effective in reducing respiratory dysfunction, vascular leak, tissue injury, and mortality during Acute Lung Injury.  We are currently validating this agent in animal models of chemical-induced ALI and testing its efficacy in reducing ALI caused by other agents such as influenza and smoke inhalation.  We hope to develop this agent as the first effective pharmacologic treatment for ALI in humans.

Development of recombinant antibodies as diagnostic reagents – Our lab has developed novel methods to generate recombinant single chain antibodies using phage display technology.  We are currently using these methods to generate pathogen-specific antibodies for use in diagnostic tests for a variety of human bacterial, viral, and fungal infections.  In collaboration with Duke Biomedical Engineering, we are testing the use of our antibodies in a novel diagnostic assay platform to develop point-of-care assays for the diagnosis of infections by agents such as Zika virus, Dengue virus, Salmonella typhi, and Aspergillus fumigatus.

Education and Training

  • Fellowship in Cardiology, Cardiology, University of California, San Francisco, 0018
  • Internship and Residency, Internal Medicine, Parkland Health & Hospital System, 0018
  • M.D., UT Southwestern Medical School, 1983
Weight
-20

Publications

Mastria, Eric M., Leon Y. Cai, Matthew J. Kan, Xinghai Li, Jeffrey L. Schaal, Steven Fiering, Michael D. Gunn, Mark W. Dewhirst, Smita K. Nair, and Ashutosh Chilkoti. “Nanoparticle formulation improves doxorubicin efficacy by enhancing host antitumor immunity..” J Control Release 269 (January 10, 2018): 364–73. https://doi.org/10.1016/j.jconrel.2017.11.021.

PMID
29146246
Full Text

Baek, Amy E., Yen-Rei A. Yu, Sisi He, Suzanne E. Wardell, Ching-Yi Chang, Sanghoon Kwon, Ruchita V. Pillai, et al. “The cholesterol metabolite 27 hydroxycholesterol facilitates breast cancer metastasis through its actions on immune cells..” Nat Commun 8, no. 1 (October 11, 2017). https://doi.org/10.1038/s41467-017-00910-z.

PMID
29021522
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Smith, J. S., L. Nicholson, J. Suwanpradid, R. Glenn, J. Gundry, P. Alagesan, N. Knape, et al. “678 Biased CXCR3 ligands differentially alter allergic contact hypersensitivity and chemotaxis.” In Journal of Investigative Dermatology, 137:S117–S117. Elsevier BV, 2017. https://doi.org/10.1016/j.jid.2017.02.701.

Full Text

Rudemiller, Nathan P., Mehul B. Patel, Jian-Dong Zhang, Alexander D. Jeffs, Norah S. Karlovich, Robert Griffiths, Matthew J. Kan, Anne F. Buckley, Michael D. Gunn, and Steven D. Crowley. “C-C Motif Chemokine 5 Attenuates Angiotensin II-Dependent Kidney Injury by Limiting Renal Macrophage Infiltration..” Am J Pathol 186, no. 11 (November 2016): 2846–56. https://doi.org/10.1016/j.ajpath.2016.07.015.

PMID
27640148
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Izykowski, Nicole, Mark Kuehnel, Kais Hussein, Kristin Mitschke, Michael Gunn, Sabina Janciauskiene, Axel Haverich, et al. “Organizing pneumonia in mice and men..” J Transl Med 14, no. 1 (June 10, 2016). https://doi.org/10.1186/s12967-016-0933-6.

PMID
27282780
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Tighe, R. M., Z. Li, E. N. Potts, S. Frush, N. Liu, M. D. Gunn, W. M. Foster, P. W. Noble, and J. W. Hollingsworth. “Erratum: Ozone inhalation promotes CX3CR1-dependent maturation of resident lung macrophages that limit oxidative stress and inflammation (Journal of Immunology (2011) 187 (4800-4808)).” Journal of Immunology 196, no. 5 (March 1, 2016). https://doi.org/10.4049/jimmunol.1600002.

Full Text

Yu, Yen-Rei A., Danielle F. Hotten, Yuryi Malakhau, Ellen Volker, Andrew J. Ghio, Paul W. Noble, Monica Kraft, John W. Hollingsworth, Michael D. Gunn, and Robert M. Tighe. “Flow Cytometric Analysis of Myeloid Cells in Human Blood, Bronchoalveolar Lavage, and Lung Tissues..” Am J Respir Cell Mol Biol 54, no. 1 (January 2016): 13–24. https://doi.org/10.1165/rcmb.2015-0146OC.

PMID
26267148
Full Text

Yu, Yen-Rei A., Emily G. O’Koren, Danielle F. Hotten, Matthew J. Kan, David Kopin, Erik R. Nelson, Loretta Que, and Michael D. Gunn. “A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues..” Plos One 11, no. 3 (2016). https://doi.org/10.1371/journal.pone.0150606.

PMID
26938654
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Kan, Matthew J., Jennifer E. Lee, Joan G. Wilson, Angela L. Everhart, Candice M. Brown, Andrew N. Hoofnagle, Marilyn Jansen, Michael P. Vitek, Michael D. Gunn, and Carol A. Colton. “Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease..” J Neurosci 35, no. 15 (April 15, 2015): 5969–82. https://doi.org/10.1523/JNEUROSCI.4668-14.2015.

PMID
25878270
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Mitchell, Duane A., Kristen A. Batich, Michael D. Gunn, Min-Nung Huang, Luis Sanchez-Perez, Smita K. Nair, Kendra L. Congdon, et al. “Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients..” Nature 519, no. 7543 (March 19, 2015): 366–69. https://doi.org/10.1038/nature14320.

PMID
25762141
Full Text

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