Michael Dee Gunn, MD

Professor of Medicine
Professor in Immunology
Associate Professor in Pathology
Campus mail 346 Sands Bldg, Durham, NC 27710
Phone (919) 681-0840
Email address michael.gunn@duke.edu

The focus of my work is on understanding how dendritic cells, monocytes, and macrophages regulate immune responses, contribute to specific disease pathologies, and can be manipulated to stimulate or inhibit specific immune responses. We are also using our knowledge of immunology to develop diagnostics and therapeutics for a variety of human diseases. 

Lab History 

The lab started with our discovery of the lymphoid chemokines, which regulate the migration of lymphocytes and dendritic cells to and within secondary lymphoid organs.  We identified the chemokine (CCL21) that mediates the entry of naïve T cells and activated dendritic cells into lymph nodes and the chemokine (CXCL13) that mediates the entry of B cells into lymphoid follicles.  Our focus then shifted to understanding how specific cell types, primarily dendritic cells, and cell migration events regulate immune responses.  We identified murine plasmacytoid dendritic cells, the cell type that causes pulmonary immune pathology during influenza infection, the dendritic cell type that stimulates Th1 immune responses, and the cell type that induces neuronal injury in Alzheimer's disease.  Our current work continues these basic studies while applying our findings to models of human disease. 

Current Research 

Identification and characterization of inflammatory cell populations in models of human disease – We have developed advanced methods of flow cytometric analysis that allows us to quantify and fully characterize all inflammatory cell types in murine and human tissues.  Using these methods, we are working to identify the cells that mediate a variety of immune pathologies.  Examples include the identification of immune-stimulatory and immune-suppressive cell types in brain tumors, identification of the cells that induce vascular changes pulmonary hypertension, and characterization of the inflammatory response to a variety of infectious pathogens.

Tumor immune therapeutics – We have developed a novel cellular vaccine strategy for the treatment of cancer.  This strategy is much simpler, more cost effective, more clinically feasible, and much more efficacious than classic dendritic cell vaccines.  We are now testing this vaccine in various preclinical tumor models including melanoma and glioblastoma and will soon be advancing it to initial human clinical trials.

Treatment of Acute Lung Injury – We have identified the first small molecule pharmacologic agent that is effective in reducing respiratory dysfunction, vascular leak, tissue injury, and mortality during Acute Lung Injury.  We are currently validating this agent in animal models of chemical-induced ALI and testing its efficacy in reducing ALI caused by other agents such as influenza and smoke inhalation.  We hope to develop this agent as the first effective pharmacologic treatment for ALI in humans.

Development of recombinant antibodies as diagnostic reagents – Our lab has developed novel methods to generate recombinant single chain antibodies using phage display technology.  We are currently using these methods to generate pathogen-specific antibodies for use in diagnostic tests for a variety of human bacterial, viral, and fungal infections.  In collaboration with Duke Biomedical Engineering, we are testing the use of our antibodies in a novel diagnostic assay platform to develop point-of-care assays for the diagnosis of infections by agents such as Zika virus, Dengue virus, Salmonella typhi, and Aspergillus fumigatus.

Education and Training

  • Fellowship in Cardiology, Cardiology, University of California - San Francisco, 1197
  • Internship and Residency, Internal Medicine, Parkland Health & Hospital System, 1197
  • M.D., UT Southwestern Medical School, 1983

Publications

Gunn, MD, Yanagita, M, and Nakano, H. "Identification and characterization of murine plasmacytoid dendritic cells." FASEB JOURNAL 16, no. 5 (March 22, 2002): A1230-A1231.

Scholars@Duke

Red-Horse, K, Drake, PM, Gunn, MD, and Fisher, SJ. "Chemokine ligand and receptor expression in the pregnant uterus: reciprocal patterns in complementary cell subsets suggest functional roles." Am J Pathol 159, no. 6 (December 2001): 2199-2213.

PMID
11733370
Full Text

Nakano, H, Yanagita, M, and Gunn, MD. "CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells." J Exp Med 194, no. 8 (October 15, 2001): 1171-1178.

PMID
11602645
Scholars@Duke

Drake, PM, Gunn, MD, Charo, IF, Tsou, CL, Zhou, Y, Huang, L, and Fisher, SJ. "Human placental cytotrophoblasts attract monocytes and CD56(bright) natural killer cells via the actions of monocyte inflammatory protein 1alpha." J Exp Med 193, no. 10 (May 21, 2001): 1199-1212.

PMID
11369791
Scholars@Duke

Mori, S, Nakano, H, Aritomi, K, Wang, CR, Gunn, MD, and Kakiuchi, T. "Mice lacking expression of the chemokines CCL21-ser and CCL19 (plt mice) demonstrate delayed but enhanced T cell immune responses." J Exp Med 193, no. 2 (January 15, 2001): 207-218.

PMID
11148224
Scholars@Duke

Nakano, H, and Gunn, MD. "Gene duplications at the chemokine locus on mouse chromosome 4: multiple strain-specific haplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mutation." J Immunol 166, no. 1 (January 1, 2001): 361-369.

PMID
11123313
Scholars@Duke

Bromley, SK, Peterson, DA, Gunn, MD, and Dustin, ML. "Cutting edge: hierarchy of chemokine receptor and TCR signals regulating T cell migration and proliferation." J Immunol 165, no. 1 (July 1, 2000): 15-19.

PMID
10861029
Scholars@Duke

Bromley, SK, Peterson, DA, Gunn, MD, and Dustin, ML. "Hierarchy of chemokine and antigen signals regulating T cell migration and proliferation." FASEB JOURNAL 14, no. 6 (April 20, 2000): A1149-A1149.

Scholars@Duke

Stein, JV, Rot, A, Luo, Y, Narasimhaswamy, M, Nakano, H, Gunn, MD, Matsuzawa, A, Quackenbush, EJ, Dorf, ME, and von Andrian, UH. "The CC chemokine thymus-derived chemotactic agent 4 (TCA-4, secondary lymphoid tissue chemokine, 6Ckine, exodus-2) triggers lymphocyte function-associated antigen 1-mediated arrest of rolling T lymphocytes in peripheral lymph node high endothelial venules." J Exp Med 191, no. 1 (January 3, 2000): 61-76.

PMID
10620605
Scholars@Duke

Bistrup, A, Bhakta, S, Lee, JK, Belov, YY, Gunn, MD, Zuo, FR, Huang, CC, Kannagi, R, Rosen, SD, and Hemmerich, S. "Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin." J Cell Biol 145, no. 4 (May 17, 1999): 899-910.

PMID
10330415
Scholars@Duke

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