Richard Frothingham, MD

Associate Professor of Medicine
Assistant Professor in Molecular Genetics and Microbiology
Member of the Duke Human Vaccine Institute
Campus mail 2424 Erwin Rd, Hock Plaza Room 9089, Durham, NC 27710
Phone (919) 684-5455
Email address richard.frothingham@duke.edu

Dr. Frothingham is the principal investigator of a research laboratory which studies Mycobacterium tuberculosis, the cause of tuberculosis, and Mycobacterium avium, a closely related bacterium causing serious infections in AIDS patients. We are pursuing two current projects.

The first project aims to develop vaccines against M. avium and M. tuberculosis. We inject mice with candidate plasmid DNA vaccines which produce bacterial proteins in mouse muscle. We use a variety of DNA adjuvants to modify the immune response. We hope to use DNA vaccination to protect against new infections and to modify the course of existing infections. We also hope to identify correlates of vaccine-induced protective immunity.

The second project uses variations in bacterial DNA sequences to identify species and strains. Dr. Frothingham was part of a team of four Duke scientists who used DNA sequence analysis to identify the cause of Whipple's disease. He also identified used DNA sequence to identify a particular group of M. avium strains which cause disseminated infections in AIDS patients. We recently developed a new tuberculosis typing method using variable numbers of tandem DNA repeats. We are applying this new typing method in national and international collaborations.

Dr. Frothingham does not currently conduct clinical trials.

Special areas of expertise include tuberculosis, mycobacteria, strain differentiation, DNA vaccination, and pyrazinamide.

Key words: tuberculosis, mycobacteria, Mycobacterium tuberculosis, Mycobacterium avium, DNA vaccines, tandem repeat DNA, pyrazinamide, mouse

Education and Training

  • Medicine and Pediatrics Resident, Medicine, University of Rochester, 1982 - 1986
  • M.D., Duke University, 1981

Publications

Marsh, B. J., C. F. von Reyn, J. Edwards, M. A. Ristola, C. Bartholomew, R. J. Brindle, C. F. Gilks, et al. “The risks and benefits of childhood bacille Calmette-Guérin immunization among adults with AIDS. International MAC study groups..” Aids (London, England) 11, no. 5 (April 1997): 669–72. https://doi.org/10.1097/00002030-199705000-00015.

Full Text

Marsh, B. J., C. F. von Reyn, J. Edwards, M. A. Ristola, C. Bartholomew, R. J. Brindle, C. F. Gilks, et al. “The risks and benefits of childhood bacille Calmette-Guérin immunization among adults with AIDS. International MAC study groups..” Aids 11, no. 5 (April 1997): 669–72. https://doi.org/10.1097/00002030-199705000-00015.

PMID
9108949
Full Text

Talbot, E. A., D. L. Williams, and R. Frothingham. “PCR identification of Mycobacterium bovis BCG..” J Clin Microbiol 35, no. 3 (March 1997): 566–69.

PMID
9041390
Scholars@Duke

Talbot, E. A., and R. Frothingham. “Meningitis due to Mycobacterium bovis BCG--reactivation or accidental intrathecal inoculation?.” Clin Infect Dis 23, no. 6 (December 1996): 1335–36. https://doi.org/10.1093/clinids/23.6.1335.

PMID
8953095
Full Text

Frothingham, R. “Book review: Drugs used in parasitic diseases, second edition..” Ann Saudi Med 16, no. 4 (July 1996): 482–83.

PMID
17372534
Scholars@Duke

Frothingham, R., W. A. Meeker-O’Connell, E. A. Talbot, J. W. George, and K. N. Kreuzer. “Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA..” Antimicrob Agents Chemother 40, no. 6 (June 1996): 1426–31.

PMID
8726014
Scholars@Duke

Frothingham, R. “Discrimination of Mycobacterium tuberculosis strains by PCR..” J Clin Microbiol 33, no. 10 (October 1995): 2801–2.

PMID
8567933
Scholars@Duke

SWANSON, D. S., V. KAPUR, X. PAN, K. STOCKBAUER, R. FROTHINGHAM, and J. M. MUSSER. “DIFFERENTIATION AMONG MYCOBACTERIUM-AVIUM COMPLEX STRAINS BY AUTOMATED SEQUENCING OF THE HSP63-GENE.” Pediatric Research 37, no. 4 (April 1, 1995): A189–A189.

Scholars@Duke

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