Richard Thomas Premont, PhD

Associate Professor in Medicine
Faculty Network Member of the Duke Institute for Brain Sciences
Campus mail 905 S. Lasalle St, Snyderman GSRB1 Room 1073D, Durham, NC 27710
Phone (919) 684-5620
Email address richard.premont@duke.edu

Critical physiological events throughout the body are controlled by extracellular signals from neurotransmitters and hormones acting on cell surface receptors. Receptors transduce these signals to alter intracellular metabolism and cellular responsiveness through heterotrimeric G protein/second messenger pathways or through small GTP-binding protein/protein kinase cascades.

The mechanisms that control the responsiveness of target organ G protein-coupled receptors include receptor phosphorylation and desensitization by G protein-coupled receptor kinases (GRKs). We have created mice lacking each of the three members of the GRK4 subfamily of GRKs (GRK4, GRK5 and GRK6), and are examining the functional effects of this loss of function on the physiology of receptors controlling gastrointestinal, heart, lung, immune and brain functions.

An important open question in the study of cellular regulation is understanding how cells coordinate the activity of multiple receptor pathways into a coherent cellular response. Our recent discovery that large multidomain ARF GTPase-activating proteins (GAPs) of the GIT family can serve as oligomeric scaffolding proteins for numerous signaling molecules and help coordinate heterotrimeric G protein and multiple small GTP-binding protein pathways, suggests that other multidomain ARF GAPs may also function in analogous manner as coordination centers. We are examining the functional roles of GIT proteins and related ARF GAPs in crosstalk among cellular signaling pathways, with particular emphasis on gastrointestinal and behavioral roles using GIT1 and GIT2 knockout mice.

Education and Training

  • Ph.D., City University of New York, 1992

Publications

Chen, W, Mook, RA, Premont, RT, and Wang, J. "Niclosamide: Beyond an antihelminthic drug." Cellular signalling 41 (January 2018): 89-96. (Review)

PMID
28389414
Full Text

Jewell, M, de Castro, GC, Premont, RT, and Diehl, AM. "Fn14 and Wnt Signaling Pathway Crosstalk Controls Liver Progenitor Expansion in 3D Organoid Culture." October 2017.

Scholars@Duke

Oh, S-H, Swiderska-Syn, M, Jewell, M, Premont, RT, and Diehl, AM. "Yap1 Activation Induces TGF-beta Signaling and Epithelial-to-Mesenchymal Transition (EMT) in Regenerating Hepatocytes After Partial Hepatectomy." October 2017.

Scholars@Duke

Lu, D, Cai, H, Park, S-S, Siddiqui, S, Premont, RT, Schmalzigaug, R, Paramasivam, M, Seidman, M, Bodogai, I, Biragyn, A, Daimon, CM, Martin, B, and Maudsley, S. "Correction for Lu et al., "Nuclear GIT2 Is an ATM Substrate and Promotes DNA Repair"." Molecular and Cellular Biology 37, no. 17 (September 2017).

PMID
28801458
Full Text

Bhattacharyya, J, Ren, X-R, Mook, RA, Wang, J, Spasojevic, I, Premont, RT, Li, X, Chilkoti, A, and Chen, W. "Niclosamide-conjugated polypeptide nanoparticles inhibit Wnt signaling and colon cancer growth." Nanoscale 9, no. 34 (August 22, 2017): 12709-12717.

PMID
28828438
Full Text

Xie, G, Swiderska-Syn, M, Jewell, ML, Machado, MV, Michelotti, GA, Premont, RT, and Diehl, AM. "Loss of pericyte smoothened activity in mice with genetic deficiency of leptin." BMC cell biology 18, no. 1 (April 20, 2017): 20-.

PMID
28427343
Full Text

Liu, S, Premont, RT, Singh, S, and Rockey, DC. "Caveolin 1 and G-Protein-Coupled Receptor Kinase-2 Coregulate Endothelial Nitric Oxide Synthase Activity in Sinusoidal Endothelial Cells." The American journal of pathology 187, no. 4 (April 2017): 896-907.

PMID
28162981
Full Text

Jung, Y, Wang, S, Friedersdorf, M, Premont, R, Oh, SH, Keene, J, and Diehl, AM. "y RNA-BINDING PROTEIN IGF2BP3 REGULATES CELL FATE DECISIONS THAT DRIVE CIRRHOSIS PATHOGENESIS." April 2017.

Scholars@Duke

Jung, Y, Wang, S, Friedersdorf, M, Premont, R, Oh, SH, Keene, J, and Diehl, AM. "RNA-Binding Protein IGF2BP3 Regulates Cell Fate Decisions that Drive Cirrhosis Pathogenesis." Gastroenterology 152, no. 5 (April 2017): S1073-S1073.

Full Text

Siddiqui, S, Lustig, A, Carter, A, Sankar, M, Daimon, CM, Premont, RT, Etienne, H, van Gastel, J, Azmi, A, Janssens, J, Becker, KG, Zhang, Y, Wood, W, Lehrmann, E, Martin, JG, Martin, B, Taub, DD, and Maudsley, S. "Genomic deletion of GIT2 induces a premature age-related thymic dysfunction and systemic immune system disruption." Aging 9, no. 3 (March 4, 2017): 706-740.

PMID
28260693
Full Text

Pages