S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Moody, M Anthony, Ruijun Zhang, Emmanuel B. Walter, Christopher W. Woods, Geoffrey S. Ginsburg, Micah T. McClain, Thomas N. Denny, et al. “H3N2 influenza infection elicits more cross-reactive and less clonally expanded anti-hemagglutinin antibodies than influenza vaccination..” Plos One 6, no. 10 (2011). https://doi.org/10.1371/journal.pone.0025797.

PMID
22039424
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Dennison, S Moses, Laura L. Sutherland, Frederick H. Jaeger, Kara M. Anasti, Robert Parks, Shelley Stewart, Cindy Bowman, et al. “Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41..” Plos One 6, no. 11 (2011). https://doi.org/10.1371/journal.pone.0027824.

PMID
22140469
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Morris, Lynn, Xi Chen, Munir Alam, Georgia Tomaras, Ruijun Zhang, Dawn J. Marshall, Bing Chen, et al. “Isolation of a human anti-HIV gp41 membrane proximal region neutralizing antibody by antigen-specific single B cell sorting..” Plos One 6, no. 9 (2011). https://doi.org/10.1371/journal.pone.0023532.

PMID
21980336
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Nicely, Nathan I., S Moses Dennison, Leonard Spicer, Richard M. Scearce, Garnett Kelsoe, Yoshihiro Ueda, Haiyan Chen, Hua-Xin Liao, S Munir Alam, and Barton F. Haynes. “Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region..” Nat Struct Mol Biol 17, no. 12 (December 2010): 1492–94. https://doi.org/10.1038/nsmb.1944.

PMID
21076400
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Saaem, I., K. S. Ma, S. M. Alam, and J. Tian. “Fabrication of plastic biochips.” Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films 28, no. 4 (July 1, 2010): 963–68. https://doi.org/10.1116/1.3432480.

Full Text

Haynes, Barton F., Nathan I. Nicely, and S Munir Alam. “HIV-1 autoreactive antibodies: are they good or bad for HIV-1 prevention?.” Nat Struct Mol Biol 17, no. 5 (May 2010): 543–45. https://doi.org/10.1038/nsmb0510-543.

PMID
20442740
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Zhang, Mei-Yun, Andrew Rosa Borges, Roger G. Ptak, Yanping Wang, Antony S. Dimitrov, S Munir Alam, Lindsay Wieczorek, et al. “Potent and broad neutralizing activity of a single chain antibody fragment against cell-free and cell-associated HIV-1..” Mabs 2, no. 3 (May 2010): 266–74. https://doi.org/10.4161/mabs.2.3.11416.

PMID
20305395
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Moody, M Anthony, Hua-Xin Liao, S Munir Alam, Richard M. Scearce, M Kelly Plonk, Daniel M. Kozink, Mark S. Drinker, et al. “Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines..” J Exp Med 207, no. 4 (April 12, 2010): 763–76. https://doi.org/10.1084/jem.20091281.

PMID
20368576
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Shen, Xiaoying, S Moses Dennison, Pinghuang Liu, Feng Gao, Frederick Jaeger, David C. Montefiori, Laurent Verkoczy, Barton F. Haynes, S Munir Alam, and Georgia D. Tomaras. “Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution..” Proc Natl Acad Sci U S A 107, no. 13 (March 30, 2010): 5972–77. https://doi.org/10.1073/pnas.0912381107.

PMID
20231447
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Verkoczy, Laurent, Marilyn Diaz, T Matt Holl, Ying-Bin Ouyang, Hilary Bouton-Verville, S Munir Alam, Hua-Xin Liao, Garnett Kelsoe, and Barton F. Haynes. “Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance..” Proc Natl Acad Sci U S A 107, no. 1 (January 5, 2010): 181–86. https://doi.org/10.1073/pnas.0912914107.

PMID
20018688
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