S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Verkoczy, L, Diaz, M, Holl, TM, Ouyang, Y, Bouton-Verville, H, Alam, SM, Liao, H, Kelsoe, G, and Haynes, BF. "P04-26. Immunological tolerance prevents the expression of a broadly reactive neutralizing HIV-1 antibody." Retrovirology 6, no. S3 (October 2009).

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Go, EP, Chang, Q, Liao, H-X, Sutherland, LL, Alam, SM, Haynes, BF, and Desaire, H. "Glycosylation site-specific analysis of clade C HIV-1 envelope proteins." J Proteome Res 8, no. 9 (September 2009): 4231-4242.

PMID
19610667
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Desaire, H, Haynes, BF, Go, EP, Liao, H, Sutherland, LL, Chang, Q, Zhang, Y, Irungu, J, and Alam, SM. "Glycosylation: an important factor in Env diversity." RETROVIROLOGY 6 (2009).

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Holl, MT, Kuraoka, M, Liao, D, Verkoczy, L, Moody, MA, Alam, SM, Haynes, B, and Kelsoe, G. "Generation of Robust Antibody Responses to HIV-1 Membrane Proximal External Region (MPER) Antigens." October 2008.

Scholars@Duke

Moody, MA, Plonk, MK, Fuller, L, Liao, H, Xia, S, Drinker, MS, Gurley, TC, Scearce, RM, Tomaras, GD, Chang, C, King, S, Kavlie, A, Thorpe, PE, Alam, SM, Chen, PP, Montefiori, DC, and Haynes, BF. "Anti-lipid Human Monoclonal Antibodies Inhibit HIV-1 Infection of PBMC by Binding to Host Cells." October 2008.

Scholars@Duke

Alam, SM, Dennison, S, Morelli, M, Liao, H, Zhang, R, Haynes, BF, Harrison, SC, and Chen, B. "The Role of Lipid Reactivity of HIV-1 gp41 Membrane Proximal Antibodies in Neutralization of HIV-1: Novel Liposome Based Immunogen Design." October 2008.

Scholars@Duke

Zhang, M-Y, Vu, BK, Choudhary, A, Lu, H, Humbert, M, Ong, H, Alam, M, Ruprecht, RM, Quinnan, G, Jiang, S, Montefiori, DC, Mascola, JR, Broder, CC, Haynes, BF, and Dimitrov, DS. "Cross-reactive human immunodeficiency virus type 1-neutralizing human monoclonal antibody that recognizes a novel conformational epitope on gp41 and lacks reactivity against self-antigens." Journal of Virology 82, no. 14 (July 2008): 6869-6879.

PMID
18480433
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Go, EP, Irungu, J, Zhang, Y, Dalpathado, DS, Liao, H-X, Sutherland, LL, Alam, SM, Haynes, BF, and Desaire, H. "Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility." Journal of Proteome Research 7, no. 4 (April 2008): 1660-1674.

PMID
18330979
Full Text

Haynes, BF, and Alam, SM. "HIV-1 hides an Achilles' heel in virion lipids." Immunity 28, no. 1 (January 2008): 10-12.

PMID
18199412
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Alam, SM, Scearce, RM, Parks, RJ, Plonk, K, Plonk, SG, Sutherland, LL, Gorny, MK, Zolla-Pazner, S, Vanleeuwen, S, Moody, MA, Xia, S-M, Montefiori, DC, Tomaras, GD, Weinhold, KJ, Karim, SA, Hicks, CB, Liao, H-X, Robinson, J, Shaw, GM, and Haynes, BF. "Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection." Journal of Virology 82, no. 1 (January 2008): 115-125.

PMID
17942537
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