S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Moody, M. A., M. K. Plonk, L. Fuller, H. Liao, S. Xia, M. S. Drinker, T. C. Gurley, et al. “Anti-lipid Human Monoclonal Antibodies Inhibit HIV-1 Infection of PBMC by Binding to Host Cells.” In Aids Research and Human Retroviruses, 24:13–13. MARY ANN LIEBERT INC, 2008.

Scholars@Duke

Alam, S. M., S. Dennison, M. Morelli, H. Liao, R. Zhang, B. F. Haynes, S. C. Harrison, and B. Chen. “The Role of Lipid Reactivity of HIV-1 gp41 Membrane Proximal Antibodies in Neutralization of HIV-1: Novel Liposome Based Immunogen Design.” In Aids Research and Human Retroviruses, 24:23–24. MARY ANN LIEBERT INC, 2008.

Scholars@Duke

Zhang, Mei-Yun, Bang K. Vu, Anil Choudhary, Hong Lu, Michael Humbert, Helena Ong, Munir Alam, et al. “Cross-reactive human immunodeficiency virus type 1-neutralizing human monoclonal antibody that recognizes a novel conformational epitope on gp41 and lacks reactivity against self-antigens..” J Virol 82, no. 14 (July 2008): 6869–79. https://doi.org/10.1128/JVI.00033-08.

PMID
18480433
Full Text

Go, Eden P., Janet Irungu, Ying Zhang, Dilusha S. Dalpathado, Hua-Xin Liao, Laura L. Sutherland, S Munir Alam, Barton F. Haynes, and Heather Desaire. “Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility..” J Proteome Res 7, no. 4 (April 2008): 1660–74. https://doi.org/10.1021/pr7006957.

PMID
18330979
Full Text

Alam, S Munir, Richard M. Scearce, Robert J. Parks, Kelly Plonk, Steven G. Plonk, Laura L. Sutherland, Miroslaw K. Gorny, et al. “Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection..” J Virol 82, no. 1 (January 2008): 115–25. https://doi.org/10.1128/JVI.00927-07.

PMID
17942537
Full Text

Lam, Y., A. Valiaev, D. W. Lim, A. Chilkoti, S. M. Alam, and S. Zauscher. “Single molecule nanomechanics of HIV-1 envelope glycoproteins and elastin-like polypeptides.” Proceedings of the Sem Annual Conference and Exposition on Experimental and Applied Mechanics 2007 1 (November 19, 2007): 624–25.

Scholars@Duke

Staats, Herman F., S Munir Alam, Richard M. Scearce, Shaun M. Kirwan, Julia Xianzhi Zhang, William M. Gwinn, and Barton F. Haynes. “In vitro and in vivo characterization of anthrax anti-protective antigen and anti-lethal factor monoclonal antibodies after passive transfer in a mouse lethal toxin challenge model to define correlates of immunity..” Infect Immun 75, no. 11 (November 2007): 5443–52. https://doi.org/10.1128/IAI.00529-07.

PMID
17709410
Full Text

Wang, Bao-Zhong, Weimin Liu, Sang-Moo Kang, Munir Alam, Chunzi Huang, Ling Ye, Yuliang Sun, et al. “Incorporation of high levels of chimeric human immunodeficiency virus envelope glycoproteins into virus-like particles..” J Virol 81, no. 20 (October 2007): 10869–78. https://doi.org/10.1128/JVI.00542-07.

PMID
17670815
Full Text

Alam, S Munir, Mildred McAdams, David Boren, Michael Rak, Richard M. Scearce, Feng Gao, Zenaido T. Camacho, et al. “The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes..” J Immunol 178, no. 7 (April 1, 2007): 4424–35. https://doi.org/10.4049/jimmunol.178.7.4424.

PMID
17372000
Full Text

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