S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Lam, Yee, Nehal I. Abu-Lail, Munir S. Alam, and Stefan Zauscher. “Using microcantilever deflection to detect HIV-1 envelope glycoprotein gp120..” Nanomedicine 2, no. 4 (December 2006): 222–29. https://doi.org/10.1016/j.nano.2006.10.002.

PMID
17292147
Full Text

Yu, Jae-Sung, Hua-Xin Liao, Aren E. Gerdon, Brian Huffman, Richard M. Scearce, Mille McAdams, S Munir Alam, et al. “Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor..” J Virol Methods 137, no. 2 (November 2006): 219–28. https://doi.org/10.1016/j.jviromet.2006.06.014.

PMID
16857271
Full Text

Liao, Hua-Xin, Laura L. Sutherland, Shi-Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S Munir Alam, et al. “A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses..” Virology 353, no. 2 (September 30, 2006): 268–82.

PMID
17039602
Scholars@Duke

Haynes, Barton F., Judith Fleming, E William St Clair, Herman Katinger, Gabriela Stiegler, Renate Kunert, James Robinson, et al. “Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies..” Science 308, no. 5730 (June 24, 2005): 1906–8. https://doi.org/10.1126/science.1111781.

PMID
15860590
Full Text

Gao, Feng, Eric A. Weaver, Zhongjing Lu, Yingying Li, Hua-Xin Liao, Benjiang Ma, S Munir Alam, et al. “Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein..” J Virol 79, no. 2 (January 2005): 1154–63. https://doi.org/10.1128/JVI.79.2.1154-1163.2005.

PMID
15613343
Full Text

Haynes, Barton F., M Anthony Moody, Laurent Verkoczy, Garnett Kelsoe, and S Munir Alam. “Antibody polyspecificity and neutralization of HIV-1: a hypothesis..” Hum Antibodies 14, no. 3–4 (2005): 59–67.

PMID
16720975
Scholars@Duke

Lam, Gordon K., Hua-Xin Liao, Yan Xue, S Munir Alam, Richard M. Scearce, Russel E. Kaufman, Gregory D. Sempowski, and Barton F. Haynes. “Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma..” J Clin Immunol 25, no. 1 (January 2005): 41–49. https://doi.org/10.1007/s10875-005-0356-5.

PMID
15742156
Full Text

Alam, S Munir, Casey A. Paleos, Hua-Xin Liao, Richard Scearce, James Robinson, and Barton F. Haynes. “An inducible HIV type 1 gp41 HR-2 peptide-binding site on HIV type 1 envelope gp120..” Aids Res Hum Retroviruses 20, no. 8 (August 2004): 836–45. https://doi.org/10.1089/0889222041725181.

PMID
15320988
Full Text

Jones, Janice C., Hemali P. Phatnani, Timothy A. Haystead, Justin A. MacDonald, S Munir Alam, and Arno L. Greenleaf. “C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats..” J Biol Chem 279, no. 24 (June 11, 2004): 24957–64. https://doi.org/10.1074/jbc.M402218200.

PMID
15047695
Full Text

Liao, Hua-Xin, S Munir Alam, John R. Mascola, James Robinson, Benjiang Ma, David C. Montefiori, Maria Rhein, Laura L. Sutherland, Richard Scearce, and Barton F. Haynes. “Immunogenicity of constrained monoclonal antibody A32-human immunodeficiency virus (HIV) Env gp120 complexes compared to that of recombinant HIV type 1 gp120 envelope glycoproteins..” J Virol 78, no. 10 (May 2004): 5270–78. https://doi.org/10.1128/jvi.78.10.5270-5278.2004.

PMID
15113908
Full Text

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