S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Haynes, BF, and Alam, SM. "HIV-1 hides an Achilles' heel in virion lipids." Immunity 28, no. 1 (January 2008): 10-12.

PMID
18199412
Full Text

Alam, SM, Scearce, RM, Parks, RJ, Plonk, K, Plonk, SG, Sutherland, LL, Gorny, MK, Zolla-Pazner, S, Vanleeuwen, S, Moody, MA, Xia, S-M, Montefiori, DC, Tomaras, GD, Weinhold, KJ, Karim, SA, Hicks, CB, Liao, H-X, Robinson, J, Shaw, GM, and Haynes, BF. "Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection." Journal of Virology 82, no. 1 (January 2008): 115-125.

PMID
17942537
Full Text

Lam, Y, Valiaev, A, Lim, DW, Chilkoti, A, Alam, SM, and Zauscher, S. "Single molecule nanomechanics of HIV-1 envelope glycoproteins and elastin-like polypeptides." Proceedings of the Sem Annual Conference and Exposition on Experimental and Applied Mechanics 2007 1 (November 19, 2007): 624-625.

Scholars@Duke

Staats, HF, Alam, SM, Scearce, RM, Kirwan, SM, Zhang, JX, Gwinn, WM, and Haynes, BF. "In vitro and in vivo characterization of anthrax anti-protective antigen and anti-lethal factor monoclonal antibodies after passive transfer in a mouse lethal toxin challenge model to define correlates of immunity." Infection and Immunity 75, no. 11 (November 2007): 5443-5452.

PMID
17709410
Full Text

Wang, B-Z, Liu, W, Kang, S-M, Alam, M, Huang, C, Ye, L, Sun, Y, Li, Y, Kothe, DL, Pushko, P, Dokland, T, Haynes, BF, Smith, G, Hahn, BH, and Compans, RW. "Incorporation of high levels of chimeric human immunodeficiency virus envelope glycoproteins into virus-like particles." Journal of Virology 81, no. 20 (October 2007): 10869-10878.

PMID
17670815
Full Text

Alam, SM, McAdams, M, Boren, D, Rak, M, Scearce, RM, Gao, F, Camacho, ZT, Gewirth, D, Kelsoe, G, Chen, P, and Haynes, BF. "The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes." Journal of Immunology (Baltimore, Md. : 1950) 178, no. 7 (April 2007): 4424-4435.

PMID
17372000
Full Text

Lam, Y, Abu-Lail, NI, Alam, MS, and Zauscher, S. "Using microcantilever deflection to detect HIV-1 envelope glycoprotein gp120." Nanomedicine 2, no. 4 (December 2006): 222-229.

PMID
17292147
Full Text

Yu, J-S, Liao, H-X, Gerdon, AE, Huffman, B, Scearce, RM, McAdams, M, Alam, SM, Popernack, PM, Sullivan, NJ, Wright, D, Cliffel, DE, Nabel, GJ, and Haynes, BF. "Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor." J Virol Methods 137, no. 2 (November 2006): 219-228.

PMID
16857271
Full Text

Liao, H-X, Sutherland, LL, Xia, S-M, Brock, ME, Scearce, RM, Vanleeuwen, S, Alam, SM, McAdams, M, Weaver, EA, Camacho, Z, Ma, B-J, Li, Y, Decker, JM, Nabel, GJ, Montefiori, DC, Hahn, BH, Korber, BT, Gao, F, and Haynes, BF. "A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses." Virology 353, no. 2 (September 2006): 268-282.

PMID
17039602
Full Text

Haynes, BF, Fleming, J, St Clair, EW, Katinger, H, Stiegler, G, Kunert, R, Robinson, J, Scearce, RM, Plonk, K, Staats, HF, Ortel, TL, Liao, H-X, and Alam, SM. "Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies." Science (New York, N.Y.) 308, no. 5730 (June 2005): 1906-1908.

PMID
15860590
Full Text

Pages