S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Lam, GK, Liao, H-X, Xue, Y, Alam, SM, Scearce, RM, Kaufman, RE, Sempowski, GD, and Haynes, BF. "Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma." J Clin Immunol 25, no. 1 (January 2005): 41-49.

PMID
15742156
Full Text

Gao, F, Weaver, EA, Lu, Z, Li, Y, Liao, H-X, Ma, B, Alam, SM, Scearce, RM, Sutherland, LL, Yu, J-S, Decker, JM, Shaw, GM, Montefiori, DC, Korber, BT, Hahn, BH, and Haynes, BF. "Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein." J Virol 79, no. 2 (January 2005): 1154-1163.

PMID
15613343
Full Text

Haynes, BF, Moody, MA, Verkoczy, L, Kelsoe, G, and Alam, SM. "Antibody polyspecificity and neutralization of HIV-1: a hypothesis." Hum Antibodies 14, no. 3-4 (2005): 59-67. (Review)

PMID
16720975
Scholars@Duke

Alam, SM, Paleos, CA, Liao, H-X, Scearce, R, Robinson, J, and Haynes, BF. "An inducible HIV type 1 gp41 HR-2 peptide-binding site on HIV type 1 envelope gp120." AIDS Res Hum Retroviruses 20, no. 8 (August 2004): 836-845.

PMID
15320988
Full Text

Jones, JC, Phatnani, HP, Haystead, TA, MacDonald, JA, Alam, SM, and Greenleaf, AL. "C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats." J Biol Chem 279, no. 24 (June 11, 2004): 24957-24964.

PMID
15047695
Full Text

Liao, H-X, Alam, SM, Mascola, JR, Robinson, J, Ma, B, Montefiori, DC, Rhein, M, Sutherland, LL, Scearce, R, and Haynes, BF. "Immunogenicity of constrained monoclonal antibody A32-human immunodeficiency virus (HIV) Env gp120 complexes compared to that of recombinant HIV type 1 gp120 envelope glycoproteins." J Virol 78, no. 10 (May 2004): 5270-5278.

PMID
15113908
Scholars@Duke

Tran, TT, Reich, CF, Alam, M, and Pisetsky, DS. "Specificity and immunochemical properties of anti-DNA antibodies induced in normal mice by immunization with mammalian DNA with a CpG oligonucleotide as adjuvant." Clin Immunol 109, no. 3 (December 2003): 278-287.

PMID
14697742
Scholars@Duke

Niederberger, N, Holmberg, K, Alam, SM, Sakati, W, Naramura, M, Gu, H, and Gascoigne, NRJ. "Allelic exclusion of the TCR alpha-chain is an active process requiring TCR-mediated signaling and c-Cbl." J Immunol 170, no. 9 (May 1, 2003): 4557-4563.

PMID
12707333
Scholars@Duke

Nickle, DC, Jensen, MA, Gottlieb, GS, Shriner, D, Learn, GH, Rodrigo, AG, and Mullins, JI. "Consensus and ancestral state HIV vaccines." Science 299, no. 5612 (March 7, 2003): 1515-1518. (Letter)

PMID
12624248
Full Text

Alam, SM, and Gascoigne, NRJ. "Binding kinetics of superantigen with TCR and MHC class II." Methods Mol Biol 214 (2003): 65-85.

PMID
12489455
Scholars@Duke

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