S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Richard, E, Alam, SM, Arredondo-Vega, FX, Patel, DD, and Hershfield, MS. "Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV." J Biol Chem 277, no. 22 (May 31, 2002): 19720-19726.

PMID
11901152
Full Text

Fong, AM, Alam, SM, Imai, T, Haribabu, B, and Patel, DD. "CX3CR1 tyrosine sulfation enhances fractalkine-induced cell adhesion." The Journal of Biological Chemistry 277, no. 22 (May 2002): 19418-19423.

PMID
11909868
Full Text

Rosette, C, Werlen, G, Daniels, MA, Holman, PO, Alam, SM, Travers, PJ, Gascoigne, NR, Palmer, E, and Jameson, SC. "The impact of duration versus extent of TCR occupancy on T cell activation: a revision of the kinetic proofreading model." Immunity 15, no. 1 (July 2001): 59-70.

PMID
11485738
Scholars@Duke

Lam, GK, Liao, HX, Xue, Y, Alam, SM, Thomasch, JR, Sempowski, GD, Stauffer, SH, Kaufman, R, and Haynes, BF. "Characterisation and expression of CD7 ligand, K-12, in human thymic epithelial cells." FASEB JOURNAL 15, no. 5 (March 8, 2001): A1033-A1033.

Scholars@Duke

Alam, SM, Paleos, C, Holmberg, K, and Gascoigne, N. "Influence of CD8 on TCR binding kinetics for agonist and antagonist ligands." FASEB JOURNAL 15, no. 5 (March 8, 2001): A1037-A1037.

Scholars@Duke

Gascoigne, NR, Zal, T, and Alam, SM. "T-cell receptor binding kinetics in T-cell development and activation. (Published online)" Expert Rev Mol Med 2001 (February 12, 2001): 1-17.

PMID
14987373
Full Text

Gascoigne, NR, Alam, SM, Lin, CM, McGuire, MV, Marine, S, Niederberger, N, Redpath, S, Sim, BC, Travers, PJ, Yachi, P, Zal, MA, and Zal, T. "T cell receptor binding kinetics and special role of Valpha in T cell development and activation." Immunol Res 21, no. 2-3 (2000): 225-231. (Review)

PMID
10852121
Full Text

Haynes, BF, Liao, HX, Staats, HF, Alam, MS, Weinhold, KJ, and Montefiori, DC. "HIV vaccine development at Duke University Medical Center." Immunologic Research 22, no. 2-3 (January 2000): 263-269. (Review)

PMID
11339361
Full Text

Gascoigne, NR, and Alam, SM. "Allelic exclusion of the T cell receptor alpha-chain: developmental regulation of a post-translational event." Semin Immunol 11, no. 5 (October 1999): 337-347. (Review)

PMID
10497088
Full Text

Redpath, S, Alam, SM, Lin, CM, O'Rourke, AM, and Gascoigne, NR. "Cutting edge: trimolecular interaction of TCR with MHC class II and bacterial superantigen shows a similar affinity to MHC:peptide ligands." J Immunol 163, no. 1 (July 1, 1999): 6-10.

PMID
10384091
Scholars@Duke

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