S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Gascoigne, N. R., S. M. Alam, C. M. Lin, M. V. McGuire, S. Marine, N. Niederberger, S. Redpath, et al. “T cell receptor binding kinetics and special role of Valpha in T cell development and activation..” Immunol Res 21, no. 2–3 (2000): 225–31. https://doi.org/10.1385/IR:21:2-3:225.

PMID
10852121
Full Text

Haynes, B. F., H. X. Liao, H. F. Staats, M. S. Alam, K. J. Weinhold, and D. C. Montefiori. “HIV vaccine development at Duke University Medical Center..” Immunol Res 22, no. 2–3 (2000): 263–69.

PMID
11339361
Scholars@Duke

Gascoigne, N. R., and S. M. Alam. “Allelic exclusion of the T cell receptor alpha-chain: developmental regulation of a post-translational event..” Semin Immunol 11, no. 5 (October 1999): 337–47. https://doi.org/10.1006/smim.1999.0190.

PMID
10497088
Full Text

Redpath, S., S. M. Alam, C. M. Lin, A. M. O’Rourke, and N. R. Gascoigne. “Cutting edge: trimolecular interaction of TCR with MHC class II and bacterial superantigen shows a similar affinity to MHC:peptide ligands..” J Immunol 163, no. 1 (July 1, 1999): 6–10.

PMID
10384091
Scholars@Duke

Alam, S. M., G. M. Davies, C. M. Lin, T. Zal, W. Nasholds, S. C. Jameson, K. A. Hogquist, N. R. Gascoigne, and P. J. Travers. “Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands..” Immunity 10, no. 2 (February 1999): 227–37. https://doi.org/10.1016/s1074-7613(00)80023-0.

PMID
10072075
Full Text

Alam, S. M., and N. R. Gascoigne. “Posttranslational regulation of TCR Valpha allelic exclusion during T cell differentiation..” J Immunol 160, no. 8 (April 15, 1998): 3883–90.

PMID
9558094
Scholars@Duke

Alam, S. M., I. Crispe, and N. Gascoigne. “Allelic exclusion of alpha-chain TCR: Analysis of dual alpha-chain expression in immature and mature thymocytes..” Journal of Allergy and Clinical Immunology 99, no. 1 (January 1997): 798–798.

Scholars@Duke

Alam, S. M., P. J. Travers, J. L. Wung, W. Nasholds, S. Redpath, S. C. Jameson, and N. R. Gascoigne. “T-cell-receptor affinity and thymocyte positive selection..” Nature 381, no. 6583 (June 13, 1996): 616–20. https://doi.org/10.1038/381616a0.

PMID
8637599
Full Text

ALAM, S. M. “T-cell-receptor affinity and thymocyte positive selection.” Nature 381 (1996).

Scholars@Duke

Alam, S. M., I. N. Crispe, and N. R. Gascoigne. “Allelic exclusion of mouse T cell receptor alpha chains occurs at the time of thymocyte TCR up-regulation..” Immunity 3, no. 4 (October 1995): 449–58. https://doi.org/10.1016/1074-7613(95)90174-4.

PMID
7584136
Full Text

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