S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Go, EP, Liao, H-X, Alam, SM, Hua, D, Haynes, BF, and Desaire, H. "Characterization of Host-Cell Line Specific Glycosylation Profiles of Early Transmitted/Founder HIV-1 gp120 Envelope Proteins." JOURNAL OF PROTEOME RESEARCH 12, no. 3 (March 2013): 1223-1234.

Full Text

Yang, G, Holl, TM, Liu, Y, Li, Y, Lu, X, Nicely, NI, Kepler, TB, Alam, SM, Liao, H-X, Cain, DW, Spicer, L, VandeBerg, JL, Haynes, BF, and Kelsoe, G. "Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies." The Journal of experimental medicine 210, no. 2 (February 2013): 241-256.

PMID
23359068
Full Text

Alam, SM, Liao, H-X, Tomaras, GD, Bonsignori, M, Tsao, C-Y, Hwang, K-K, Chen, H, Lloyd, KE, Bowman, C, Sutherland, L, Jeffries, TL, Kozink, DM, Stewart, S, Anasti, K, Jaeger, FH, Parks, R, Yates, NL, Overman, RG, Sinangil, F, Berman, PW, Pitisuttithum, P, Kaewkungwal, J, Nitayaphan, S, Karasavva, N, Rerks-Ngarm, S, Kim, JH, Michael, NL, Zolla-Pazner, S, Santra, S, Letvin, NL, Harrison, SC, and Haynes, BF. "Antigenicity and immunogenicity of RV144 vaccine AIDSVAX clade E envelope immunogen is enhanced by a gp120 N-terminal deletion." J Virol 87, no. 3 (February 2013): 1554-1568.

PMID
23175357
Full Text

Pegu, P, Vaccari, M, Gordon, S, Keele, BF, Doster, M, Guan, Y, Ferrari, G, Pal, R, Ferrari, MG, Whitney, S, Hudacik, L, Billings, E, Rao, M, Montefiori, D, Tomaras, G, Alam, SM, Fenizia, C, Lifson, JD, Stablein, D, Tartaglia, J, Michael, N, Kim, J, Venzon, D, and Franchini, G. "Antibodies with high avidity to the gp120 envelope protein in protection from simian immunodeficiency virus SIV(mac251) acquisition in an immunization regimen that mimics the RV-144 Thai trial." J Virol 87, no. 3 (February 2013): 1708-1719.

PMID
23175374
Full Text

Liao, H-X, Bonsignori, M, Alam, SM, McLellan, JS, Tomaras, GD, Moody, MA, Kozink, DM, Hwang, K-K, Chen, X, Tsao, C-Y, Liu, P, Lu, X, Parks, RJ, Montefiori, DC, Ferrari, G, Pollara, J, Rao, M, Peachman, KK, Santra, S, Letvin, NL, Karasavvas, N, Yang, Z-Y, Dai, K, Pancera, M, Gorman, J, Wiehe, K, Nicely, NI, Rerks-Ngarm, S, Nitayaphan, S, Kaewkungwal, J, Pitisuttithum, P, Tartaglia, J, Sinangil, F, Kim, JH, Michael, NL, Kepler, TB, Kwong, PD, Mascola, JR, Nabel, GJ, Pinter, A, and Zolla-Pazner, S et al. "Vaccine induction of antibodies against a structurally heterogeneous site of immune pressure within HIV-1 envelope protein variable regions 1 and 2." Immunity 38, no. 1 (January 11, 2013): 176-186.

PMID
23313589
Full Text

Fouda, GG, Mahlokozera, T, Salazar-Gonzalez, JF, Salazar, MG, Learn, G, Kumar, SB, Dennison, SM, Russell, E, Rizzolo, K, Jaeger, F, Cai, F, Vandergrift, NA, Gao, F, Hahn, B, Shaw, GM, Ochsenbauer, C, Swanstrom, R, Meshnick, S, Mwapasa, V, Kalilani, L, Fiscus, S, Montefiori, D, Haynes, B, Kwiek, J, Alam, SM, and Permar, SR. "Postnatally-transmitted HIV-1 Envelope variants have similar neutralization-sensitivity and function to that of nontransmitted breast milk variants." Retrovirology 10 (January 10, 2013): 3-.

PMID
23305422
Full Text

Pancera, M, Shahzad-Ul-Hussan, S, Doria-Rose, NA, McLellan, JS, Bailer, RT, Dai, K, Loesgen, S, Louder, MK, Staupe, RP, Yang, Y, Zhang, B, Parks, R, Eudailey, J, Lloyd, KE, Blinn, J, Alam, SM, Haynes, BF, Amin, MN, Wang, L-X, Burton, DR, Koff, WC, Nabel, GJ, Mascola, JR, Bewley, CA, and Kwong, PD. "Structural basis for diverse N-glycan recognition by HIV-1-neutralizing V1-V2-directed antibody PG16." Nature Structural and Molecular Biology 20, no. 7 (2013): 804-813.

Full Text

Huang, J, Ofek, G, Laub, L, Louder, MK, Doria-Rose, NA, Longo, NS, Imamichi, H, Bailer, RT, Chakrabarti, B, Sharma, SK, Alam, SM, Wang, T, Yang, Y, Zhang, B, Migueles, SA, Wyatt, R, Haynes, BF, Kwong, PD, Mascola, JR, and Connors, M. "Broad and potent neutralization of HIV-1 by a gp41-specific human antibody." Nature 491, no. 7424 (November 15, 2012): 406-412.

PMID
23151583
Full Text

Bonsignori, M, Alam, SM, Liao, H-X, Verkoczy, L, Tomaras, GD, Haynes, BF, and Moody, MA. "HIV-1 antibodies from infection and vaccination: insights for guiding vaccine design." Trends Microbiol 20, no. 11 (November 2012): 532-539. (Review)

PMID
22981828
Full Text

Petrovas, C, Yamamoto, T, Gerner, MY, Boswell, KL, Wloka, K, Smith, EC, Ambrozak, DR, Sandler, NG, Timmer, KJ, Sun, X, Pan, L, Poholek, A, Rao, SS, Brenchley, JM, Alam, SM, Tomaras, GD, Roederer, M, Douek, DC, Seder, RA, Germain, RN, Haddad, EK, and Koup, RA. "CD4 T follicular helper cell dynamics during SIV infection." J Clin Invest 122, no. 9 (September 2012): 3281-3294.

PMID
22922258
Full Text

Pages