S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Zhang, J, Alam, SM, Bouton-Verville, H, Chen, Y, Newman, A, Stewart, S, Jaeger, FH, Montefiori, DC, Dennison, SM, Haynes, BF, and Verkoczy, L. "Modulation of nonneutralizing HIV-1 gp41 responses by an MHC-restricted TH epitope overlapping those of membrane proximal external region broadly neutralizing antibodies." Journal of Immunology 192, no. 4 (February 15, 2014): 1693-1706.

Full Text

Lynch, HE, Stewart, SM, Kepler, TB, Sempowski, GD, and Alam, SM. "Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen." Journal of Immunological Methods 404, no. 1 (February 1, 2014): 1-12.

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Lynch, HE, Stewart, SM, Kepler, TB, Sempowski, GD, and Alam, SM. "Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen." J Immunol Methods 404 (February 2014): 1-12.

PMID
24316020
Full Text

Zhang, J, Alam, SM, Bouton-Verville, H, Chen, Y, Newman, A, Stewart, S, Jaeger, FH, Montefiori, DC, Dennison, SM, Haynes, BF, and Verkoczy, L. "Modulation of nonneutralizing HIV-1 gp41 responses by an MHC-restricted TH epitope overlapping those of membrane proximal external region broadly neutralizing antibodies." Journal of immunology (Baltimore, Md. : 1950) 192, no. 4 (February 2014): 1693-1706.

PMID
24465011
Full Text

Hu, B, Liao, H-X, Alam, SM, and Goldstein, B. "Estimating the probability of polyreactive antibodies 4E10 and 2F5 disabling a gp41 trimer after T cell-HIV adhesion." PLoS computational biology 10, no. 1 (January 30, 2014): e1003431-.

PMID
24499928
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Reardon, PN, Sage, H, Dennison, SM, Martin, JW, Donald, BR, Alam, SM, Haynes, BF, and Spicer, LD. "Structure of an HIV-1-neutralizing antibody target, the lipid-bound gp41 envelope membrane proximal region trimer." Proceedings of the National Academy of Sciences of the United States of America 111, no. 4 (January 13, 2014): 1391-1396.

PMID
24474763
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Kepler, TB, Munshaw, S, Wiehe, K, Zhang, R, Yu, J-S, Woods, CW, Denny, TN, Tomaras, GD, Alam, SM, Moody, MA, Kelsoe, G, Liao, H-X, and Haynes, BF. "Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation." Frontiers in Immunology 5 (January 2014): 170-null.

PMID
24795717
Full Text

Lynch, HE, Stewart, SM, Kepler, TB, Sempowski, GD, and Alam, SM. "Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen." Journal of Immunological Methods 404, no. 1 (2014): 1-12.

Scholars@Duke

Roederer, M, Keele, BF, Schmidt, SD, Mason, RD, Welles, HC, Fischer, W, Labranche, C, Foulds, KE, Louder, MK, Yang, ZY, Todd, JPM, Buzby, AP, Mach, LV, Shen, L, Seaton, KE, Ward, BM, Bailer, RT, Gottardo, R, Gu, W, Ferrari, G, Alam, SM, Denny, TN, Montefiori, DC, Tomaras, GD, Korber, BT, Nason, MC, Seder, RA, Koup, RA, Letvin, NL, Rao, SS, Nabel, GJ, and Mascola, JR. "Immunological and virological mechanisms of vaccine-mediated protection against SIV and HIV." Nature 505, no. 7484 (January 1, 2014): 502-508.

Full Text

Hu, B, Liao, HX, Alam, SM, and Goldstein, B. "Estimating the Probability of Polyreactive Antibodies 4E10 and 2F5 Disabling a gp41 Trimer after T Cell-HIV Adhesion." Plos Computational Biology 10, no. 1 (January 1, 2014).

Full Text

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