S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Trama, Ashley M., M Anthony Moody, S Munir Alam, Frederick H. Jaeger, Bradley Lockwood, Robert Parks, Krissey E. Lloyd, et al. “HIV-1 envelope gp41 antibodies can originate from terminal ileum B cells that share cross-reactivity with commensal bacteria..” Cell Host Microbe 16, no. 2 (August 13, 2014): 215–26. https://doi.org/10.1016/j.chom.2014.07.003.

PMID
25121750
Full Text

Dennison, S Moses, Kara M. Anasti, Frederick H. Jaeger, Shelley M. Stewart, Justin Pollara, Pinghuang Liu, Erika L. Kunz, et al. “Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide..” J Virol 88, no. 16 (August 2014): 9406–17. https://doi.org/10.1128/JVI.01031-14.

PMID
24920809
Full Text

Gao, Feng, Mattia Bonsignori, Hua-Xin Liao, Amit Kumar, Shi-Mao Xia, Xiaozhi Lu, Fangping Cai, et al. “Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies..” Cell 158, no. 3 (July 31, 2014): 481–91. https://doi.org/10.1016/j.cell.2014.06.022.

PMID
25065977
Full Text

Pollara, Justin, Mattia Bonsignori, M Anthony Moody, Pinghuang Liu, S Munir Alam, Kwan-Ki Hwang, Thaddeus C. Gurley, et al. “HIV-1 vaccine-induced C1 and V2 Env-specific antibodies synergize for increased antiviral activities..” J Virol 88, no. 14 (July 2014): 7715–26. https://doi.org/10.1128/JVI.00156-14.

PMID
24807721
Full Text

Haynes, Barton F., M Anthony Moody, Munir Alam, Mattia Bonsignori, Laurent Verkoczy, Guido Ferrari, Feng Gao, Georgia D. Tomaras, Hua-Xin Liao, and Garnett Kelsoe. “Progress in HIV-1 vaccine development..” J Allergy Clin Immunol 134, no. 1 (July 2014): 3–10. https://doi.org/10.1016/j.jaci.2014.04.025.

PMID
25117798
Full Text

Holl, T Matt, Guang Yang, Masayuki Kuraoka, Laurent Verkoczy, S Munir Alam, M Anthony Moody, Barton F. Haynes, and Garnett Kelsoe. “Enhanced antibody responses to an HIV-1 membrane-proximal external region antigen in mice reconstituted with cultured lymphocytes..” J Immunol 192, no. 7 (April 1, 2014): 3269–79. https://doi.org/10.4049/jimmunol.1302829.

PMID
24591365
Full Text

Osuna, Christa E., Ana Maria Gonzalez, Hsun-Hsien Chang, Amy Shi Hung, Elizabeth Ehlinger, Kara Anasti, S Munir Alam, and Norman L. Letvin. “TCR affinity associated with functional differences between dominant and subdominant SIV epitope-specific CD8+ T cells in Mamu-A*01+ rhesus monkeys..” Plos Pathog 10, no. 4 (April 2014). https://doi.org/10.1371/journal.ppat.1004069.

PMID
24743648
Full Text

Yates, Nicole L., Hua-Xin Liao, Youyi Fong, Allan deCamp, Nathan A. Vandergrift, William T. Williams, S Munir Alam, et al. “Vaccine-induced Env V1-V2 IgG3 correlates with lower HIV-1 infection risk and declines soon after vaccination..” Sci Transl Med 6, no. 228 (March 19, 2014). https://doi.org/10.1126/scitranslmed.3007730.

PMID
24648342
Full Text

Moody, M Anthony, Sampa Santra, Nathan A. Vandergrift, Laura L. Sutherland, Thaddeus C. Gurley, Mark S. Drinker, Ashley A. Allen, et al. “Toll-like receptor 7/8 (TLR7/8) and TLR9 agonists cooperate to enhance HIV-1 envelope antibody responses in rhesus macaques..” J Virol 88, no. 6 (March 2014): 3329–39. https://doi.org/10.1128/JVI.03309-13.

PMID
24390332
Full Text

Zhang, Jinsong, S Munir Alam, Hilary Bouton-Verville, Yao Chen, Amanda Newman, Shelley Stewart, Frederick H. Jaeger, et al. “Modulation of nonneutralizing HIV-1 gp41 responses by an MHC-restricted TH epitope overlapping those of membrane proximal external region broadly neutralizing antibodies..” J Immunol 192, no. 4 (February 15, 2014): 1693–1706. https://doi.org/10.4049/jimmunol.1302511.

PMID
24465011
Full Text

Pages