S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Liu, P, Yates, NL, Shen, X, Bonsignori, M, Moody, MA, Liao, H-X, Fong, Y, Alam, SM, Overman, RG, Denny, T, Ferrari, G, Ochsenbauer, C, Kappes, JC, Polonis, VR, Pitisuttithum, P, Kaewkungwal, J, Nitayaphan, S, Rerks-Ngarm, S, Montefiori, DC, Gilbert, P, Michael, NL, Kim, JH, Haynes, BF, and Tomaras, GD. "Infectious virion capture by HIV-1 gp120-specific IgG from RV144 vaccinees." J Virol 87, no. 14 (July 2013): 7828-7836.

PMID
23658446
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Pancera, M, Shahzad-Ul-Hussan, S, Doria-Rose, NA, McLellan, JS, Bailer, RT, Dai, K, Loesgen, S, Louder, MK, Staupe, RP, Yang, Y, Zhang, B, Parks, R, Eudailey, J, Lloyd, KE, Blinn, J, Alam, SM, Haynes, BF, Amin, MN, Wang, L-X, Burton, DR, Koff, WC, Nabel, GJ, Mascola, JR, Bewley, CA, and Kwong, PD. "Structural basis for diverse N-glycan recognition by HIV-1-neutralizing V1-V2-directed antibody PG16." Nat Struct Mol Biol 20, no. 7 (July 2013): 804-813.

PMID
23708607
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Tomaras, GD, Ferrari, G, Shen, X, Alam, SM, Liao, H-X, Pollara, J, Bonsignori, M, Moody, MA, Fong, Y, Chen, X, Poling, B, Nicholson, CO, Zhang, R, Lu, X, Parks, R, Kaewkungwal, J, Nitayaphan, S, Pitisuttithum, P, Rerks-Ngarm, S, Gilbert, PB, Kim, JH, Michael, NL, Montefiori, DC, and Haynes, BF. "Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG." Proc Natl Acad Sci U S A 110, no. 22 (May 28, 2013): 9019-9024.

PMID
23661056
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Liao, H-X, Lynch, R, Zhou, T, Gao, F, Alam, SM, Boyd, SD, Fire, AZ, Roskin, KM, Schramm, CA, Zhang, Z, Zhu, J, Shapiro, L, NISC Comparative Sequencing Program, , Mullikin, JC, Gnanakaran, S, Hraber, P, Wiehe, K, Kelsoe, G, Yang, G, Xia, S-M, Montefiori, DC, Parks, R, Lloyd, KE, Scearce, RM, Soderberg, KA, Cohen, M, Kamanga, G, Louder, MK, Tran, LM, Chen, Y, Cai, F, Chen, S, Moquin, S, Du, X, Joyce, MG, Srivatsan, S, Zhang, B, Zheng, A, Shaw, GM, Hahn, BH, Kepler, TB, Korber, BTM, Kwong, PD, Mascola, JR, and Haynes, BF. "Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus." Nature 496, no. 7446 (April 25, 2013): 469-476.

PMID
23552890
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Zhu, J, Ofek, G, Yang, Y, Zhang, B, Louder, MK, Lu, G, McKee, K, Pancera, M, Skinner, J, Zhang, Z, Parks, R, Eudailey, J, Lloyd, KE, Blinn, J, Alam, SM, Haynes, BF, Simek, M, Burton, DR, Koff, WC, NISC Comparative Sequencing Program, , Mullikin, JC, Mascola, JR, Shapiro, L, and Kwong, PD. "Mining the antibodyome for HIV-1-neutralizing antibodies with next-generation sequencing and phylogenetic pairing of heavy/light chains." Proc Natl Acad Sci U S A 110, no. 16 (April 16, 2013): 6470-6475.

PMID
23536288
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Liao, H-X, Tsao, C-Y, Alam, SM, Muldoon, M, Vandergrift, N, Ma, B-J, Lu, X, Sutherland, LL, Scearce, RM, Bowman, C, Parks, R, Chen, H, Blinn, JH, Lapedes, A, Watson, S, Xia, S-M, Foulger, A, Hahn, BH, Shaw, GM, Swanstrom, R, Montefiori, DC, Gao, F, Haynes, BF, and Korber, B. "Antigenicity and immunogenicity of transmitted/founder, consensus, and chronic envelope glycoproteins of human immunodeficiency virus type 1." J Virol 87, no. 8 (April 2013): 4185-4201.

PMID
23365441
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Go, EP, Liao, H-X, Alam, SM, Hua, D, Haynes, BF, and Desaire, H. "Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins." J Proteome Res 12, no. 3 (March 1, 2013): 1223-1234.

PMID
23339644
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Go, EP, Liao, H-X, Alam, SM, Hua, D, Haynes, BF, and Desaire, H. "Characterization of Host-Cell Line Specific Glycosylation Profiles of Early Transmitted/Founder HIV-1 gp120 Envelope Proteins." Journal of Proteome Research 12, no. 3 (March 2013): 1223-1234.

Full Text

Yang, G, Holl, TM, Liu, Y, Li, Y, Lu, X, Nicely, NI, Kepler, TB, Alam, SM, Liao, H-X, Cain, DW, Spicer, L, VandeBerg, JL, Haynes, BF, and Kelsoe, G. "Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies." J Exp Med 210, no. 2 (February 11, 2013): 241-256.

PMID
23359068
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Alam, SM, Liao, H-X, Tomaras, GD, Bonsignori, M, Tsao, C-Y, Hwang, K-K, Chen, H, Lloyd, KE, Bowman, C, Sutherland, L, Jeffries, TL, Kozink, DM, Stewart, S, Anasti, K, Jaeger, FH, Parks, R, Yates, NL, Overman, RG, Sinangil, F, Berman, PW, Pitisuttithum, P, Kaewkungwal, J, Nitayaphan, S, Karasavva, N, Rerks-Ngarm, S, Kim, JH, Michael, NL, Zolla-Pazner, S, Santra, S, Letvin, NL, Harrison, SC, and Haynes, BF. "Antigenicity and immunogenicity of RV144 vaccine AIDSVAX clade E envelope immunogen is enhanced by a gp120 N-terminal deletion." J Virol 87, no. 3 (February 2013): 1554-1568.

PMID
23175357
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