S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Fouda, GG, Jaeger, FH, Amos, JD, Ho, C, Kunz, EL, Anasti, K, Stamper, LW, Liebl, BE, Barbas, KH, Ohashi, T, Moseley, MA, Liao, H-X, Erickson, HP, Alam, SM, and Permar, SR. "Tenascin-C is an innate broad-spectrum, HIV-1-neutralizing protein in breast milk." Proc Natl Acad Sci U S A 110, no. 45 (November 5, 2013): 18220-18225.

PMID
24145401
Full Text

Alam, SM, Dennison, SM, Aussedat, B, Vohra, Y, Park, PK, Fernández-Tejada, A, Stewart, S, Jaeger, FH, Anasti, K, Blinn, JH, Kepler, TB, Bonsignori, M, Liao, H-X, Sodroski, JG, Danishefsky, SJ, and Haynes, BF. "Recognition of synthetic glycopeptides by HIV-1 broadly neutralizing antibodies and their unmutated ancestors." Proceedings of the National Academy of Sciences of the United States of America 110, no. 45 (November 2013): 18214-18219.

PMID
24145434
Full Text

Gao, F, Bonsignori, M, Liao, H, Kumar, A, Xia, S, Cai, F, Lu, X, Kozink, DM, Kwong, P, Zhou, T, Lynch, R, Alam, SM, Ferrari, G, Kelsoe, G, Sandrasegaram, G, Shaw, GM, Hahn, BH, Montefiori, DC, Kamanga, G, Cohen, M, Braber, P, Korber, BT, Mascola, JR, Kepler, TB, and Haynes, BF. "Multiple Pathways of HIV-1 Autologous Neutralizing Antibodies Cooperate to Drive CD4 Binding Site Broadly Neutralizing Antibody Responses." November 1, 2013.

Scholars@Duke

Chen, Y, Zhang, J, Bouton-Verville, H, Newman, A, Xia, S, Liao, H, Montefiori, DC, Moody, MA, Armand, L, Hwang, K, Lockwood, B, Dennison, SM, Alam, SM, Haynes, BF, and Verkoczy, L. "Lipid Components in MPER-Based Immunization Regimens Are Critical for Inducing Broadly Neutralizing Antibody Responses in 2F5 and 4E10 Knockin Mice." November 1, 2013.

Scholars@Duke

Nicely, NI, Wiehe, K, Kepler, TB, Jaeger, FH, Dennison, SM, Liao, H, Alam, SM, Rerks-Ngarm, S, Nitayaphan, S, Pitisuttithum, P, Kaewkungwal, J, Michael, NL, Kim, JH, and Haynes, BF. "Structural Analysis of the Unmutated Common Ancestor Antibodies of the HIV Envelope V2 Antibodies CH58 and CH59 Derived from RV144 Vaccinees." November 1, 2013.

Scholars@Duke

Trama, AM, Liao, H, Jaeger, FH, Alam, SM, Wiehe, K, Jr, JTL, Foulger, A, Williams, WT, Marshall, DJ, Stolarchuk, C, Lloyd, KE, Parks, R, Soderberg, K, Kepler, TB, Vandergrift, N, Jackson, KJ, Fire, AZ, Boyd, SD, Whitesides, JF, Tomaras, GD, Moody, MA, and Haynes, BF. "Intestinal Commensal Bacteria Shape the Pre-transmission Antibody Repertoire to HIV-1 Infection." November 1, 2013.

Scholars@Duke

Verkoczy, L, Chen, Y, Zhang, J, Bouton-Verville, H, Newman, A, Lockwood, B, Scearce, RM, Montefiori, DC, Dennison, SM, Xia, S-M, Hwang, K-K, Liao, H-X, Alam, SM, and Haynes, BF. "Induction of HIV-1 broad neutralizing antibodies in 2F5 knock-in mice: selection against membrane proximal external region-associated autoreactivity limits T-dependent responses." Journal of Immunology (Baltimore, Md. : 1950) 191, no. 5 (September 2013): 2538-2550.

PMID
23918977
Full Text

Aussedat, B, Vohra, Y, Park, PK, Fernández-Tejada, A, Alam, SM, Dennison, SM, Jaeger, FH, Anasti, K, Stewart, S, Blinn, JH, Liao, H-X, Sodroski, JG, Haynes, BF, and Danishefsky, SJ. "Chemical synthesis of highly congested gp120 V1V2 N-glycopeptide antigens for potential HIV-1-directed vaccines." Journal of the American Chemical Society 135, no. 35 (September 2013): 13113-13120.

PMID
23915436
Full Text

Liu, P, Yates, NL, Shen, X, Bonsignori, M, Moody, MA, Liao, H-X, Fong, Y, Alam, SM, Overman, RG, Denny, T, Ferrari, G, Ochsenbauer, C, Kappes, JC, Polonis, VR, Pitisuttithum, P, Kaewkungwal, J, Nitayaphan, S, Rerks-Ngarm, S, Montefiori, DC, Gilbert, P, Michael, NL, Kim, JH, Haynes, BF, and Tomaras, GD. "Infectious virion capture by HIV-1 gp120-specific IgG from RV144 vaccinees." J Virol 87, no. 14 (July 2013): 7828-7836.

PMID
23658446
Full Text

Pancera, M, Shahzad-Ul-Hussan, S, Doria-Rose, NA, McLellan, JS, Bailer, RT, Dai, K, Loesgen, S, Louder, MK, Staupe, RP, Yang, Y, Zhang, B, Parks, R, Eudailey, J, Lloyd, KE, Blinn, J, Alam, SM, Haynes, BF, Amin, MN, Wang, L-X, Burton, DR, Koff, WC, Nabel, GJ, Mascola, JR, Bewley, CA, and Kwong, PD. "Structural basis for diverse N-glycan recognition by HIV-1-neutralizing V1-V2-directed antibody PG16." Nat Struct Mol Biol 20, no. 7 (July 2013): 804-813.

PMID
23708607
Full Text

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