S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Huang, Jinghe, Gilad Ofek, Leo Laub, Mark K. Louder, Nicole A. Doria-Rose, Nancy S. Longo, Hiromi Imamichi, et al. “Broad and potent neutralization of HIV-1 by a gp41-specific human antibody..” Nature 491, no. 7424 (November 15, 2012): 406–12. https://doi.org/10.1038/nature11544.

PMID
23151583
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Bonsignori, Mattia, S Munir Alam, Hua-Xin Liao, Laurent Verkoczy, Georgia D. Tomaras, Barton F. Haynes, and M Anthony Moody. “HIV-1 antibodies from infection and vaccination: insights for guiding vaccine design..” Trends Microbiol 20, no. 11 (November 2012): 532–39. https://doi.org/10.1016/j.tim.2012.08.011.

PMID
22981828
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Petrovas, Constantinos, Takuya Yamamoto, Michael Y. Gerner, Kristin L. Boswell, Kaska Wloka, Emily C. Smith, David R. Ambrozak, et al. “CD4 T follicular helper cell dynamics during SIV infection..” J Clin Invest 122, no. 9 (September 2012): 3281–94. https://doi.org/10.1172/JCI63039.

PMID
22922258
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Moody, M Anthony, Nicole L. Yates, Joshua D. Amos, Mark S. Drinker, Joshua A. Eudailey, Thaddeus C. Gurley, Dawn J. Marshall, et al. “HIV-1 gp120 vaccine induces affinity maturation in both new and persistent antibody clonal lineages..” J Virol 86, no. 14 (July 2012): 7496–7507. https://doi.org/10.1128/JVI.00426-12.

PMID
22553329
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Haynes, Barton F., Peter B. Gilbert, M Juliana McElrath, Susan Zolla-Pazner, Georgia D. Tomaras, S Munir Alam, David T. Evans, et al. “Immune-correlates analysis of an HIV-1 vaccine efficacy trial..” N Engl J Med 366, no. 14 (April 5, 2012): 1275–86. https://doi.org/10.1056/NEJMoa1113425.

PMID
22475592
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Hardy, Gregory J., Yee Lam, Shelley M. Stewart, Kara Anasti, S Munir Alam, and Stefan Zauscher. “Screening the interactions between HIV-1 neutralizing antibodies and model lipid surfaces..” J Immunol Methods 376, no. 1–2 (February 28, 2012): 13–19. https://doi.org/10.1016/j.jim.2011.10.005.

PMID
22033342
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Friedman, James, S Munir Alam, Xiaoying Shen, Shi-Mao Xia, Shelley Stewart, Kara Anasti, Justin Pollara, et al. “Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum..” Plos One 7, no. 5 (2012). https://doi.org/10.1371/journal.pone.0037648.

PMID
22624058
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Tomaras, Georgia D., James M. Binley, Elin S. Gray, Emma T. Crooks, Keiko Osawa, Penny L. Moore, Nancy Tumba, et al. “Polyclonal B cell responses to conserved neutralization epitopes in a subset of HIV-1-infected individuals..” J Virol 85, no. 21 (November 2011): 11502–19. https://doi.org/10.1128/JVI.05363-11.

PMID
21849452
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Liu, Pinghuang, R Glenn Overman, Nicole L. Yates, S Munir Alam, Nathan Vandergrift, Yue Chen, Frederik Graw, et al. “Dynamic antibody specificities and virion concentrations in circulating immune complexes in acute to chronic HIV-1 infection..” J Virol 85, no. 21 (November 2011): 11196–207. https://doi.org/10.1128/JVI.05601-11.

PMID
21865397
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Alam, S Munir, Hua-Xin Liao, S Moses Dennison, Frederick Jaeger, Robert Parks, Kara Anasti, Andrew Foulger, et al. “Differential reactivity of germ line allelic variants of a broadly neutralizing HIV-1 antibody to a gp41 fusion intermediate conformation..” J Virol 85, no. 22 (November 2011): 11725–31. https://doi.org/10.1128/JVI.05680-11.

PMID
21917975
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