S. Munir Alam, PhD

Professor in Medicine
Professor of Pathology
Member of the Duke Human Vaccine Institute
Campus mail 2 Genome Ct, MSRB II - Room 4004, Durham, NC 27710
Phone (919) 668-6372
Email address alam0004@mc.duke.edu

Research Interests.
Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.

Education and Training

  • Ph.D., University of Glasgow (Scotland), 1992

Publications

Shen, X, Basu, R, Sawant, S, Beaumont, D, Kwa, SF, LaBranche, C, Seaton, KE, Yates, NL, Montefiori, DC, Ferrari, G, Wyatt, LS, Moss, B, Alam, SM, Haynes, BF, Tomaras, GD, and Robinson, HL. "HIV-1 gp120 and Modified Vaccinia Virus Ankara (MVA) gp140 Boost Immunogens Increase Immunogenicity of a DNA/MVA HIV-1 Vaccine." Journal of Virology 91, no. 24 (December 2017).

PMID
29021394
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Saunders, KO, Verkoczy, LK, Jiang, C, Zhang, J, Parks, R, Chen, H, Housman, M, Bouton-Verville, H, Shen, X, Trama, AM, Scearce, R, Sutherland, L, Santra, S, Newman, A, Eaton, A, Xu, K, Georgiev, IS, Joyce, MG, Tomaras, GD, Bonsignori, M, Reed, SG, Salazar, A, Mascola, JR, Moody, MA, Cain, DW, Centlivre, M, Zurawski, S, Zurawski, G, Erickson, HP, Kwong, PD, Alam, SM, Levy, Y, Montefiori, DC, and Haynes, BF. "Vaccine Induction of Heterologous Tier 2 HIV-1 Neutralizing Antibodies in Animal Models." Cell Reports 21, no. 13 (December 2017): 3681-3690.

PMID
29281818
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Williams, WB, Zhang, J, Jiang, C, Nicely, NI, Fera, D, Luo, K, Moody, MA, Liao, H-X, Alam, SM, Kepler, TB, Ramesh, A, Wiehe, K, Holland, JA, Bradley, T, Vandergrift, N, Saunders, KO, Parks, R, Foulger, A, Xia, S-M, Bonsignori, M, Montefiori, DC, Louder, M, Eaton, A, Santra, S, Scearce, R, Sutherland, L, Newman, A, Bouton-Verville, H, Bowman, C, Bomze, H, Gao, F, Marshall, DJ, Whitesides, JF, Nie, X, Kelsoe, G, Reed, SG, Fox, CB, Clary, K, Koutsoukos, M, Franco, D, Mascola, JR, Harrison, SC, Haynes, BF, and Verkoczy, L. "Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations." Nature Communications 8, no. 1 (November 23, 2017): 1732-null.

PMID
29170366
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Shen, X, Bogers, WM, Yates, NL, Ferrari, G, Dey, AK, Williams, WT, Jaeger, FH, Wiehe, K, Sawant, S, Alam, SM, LaBranche, CC, Montefiori, DC, Martin, L, Srivastava, I, Heeney, J, Barnett, SW, and Tomaras, GD. "Cross-Linking of a CD4-Mimetic Miniprotein with HIV-1 Env gp140 Alters Kinetics and Specificities of Antibody Responses against HIV-1 Env in Macaques." Journal of Virology 91, no. 19 (October 2017).

PMID
28490585
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Meyerhoff, RR, Scearce, RM, Ogburn, DF, Lockwood, B, Pickeral, J, Kuraoka, M, Anasti, K, Eudailey, J, Eaton, A, Cooper, M, Wiehe, K, Montefiori, DC, Tomaras, G, Ferrari, G, Alam, SM, Liao, H-X, Korber, B, Gao, F, and Haynes, BF. "HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies." AIDS research and human retroviruses 33, no. 8 (August 2017): 859-868.

PMID
28314374
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Bonsignori, M, Kreider, EF, Fera, D, Meyerhoff, RR, Bradley, T, Wiehe, K, Alam, SM, Aussedat, B, Walkowicz, WE, Hwang, K-K, Saunders, KO, Zhang, R, Gladden, MA, Monroe, A, Kumar, A, Xia, S-M, Cooper, M, Louder, MK, McKee, K, Bailer, RT, Pier, BW, Jette, CA, Kelsoe, G, Williams, WB, Morris, L, Kappes, J, Wagh, K, Kamanga, G, Cohen, MS, Hraber, PT, Montefiori, DC, Trama, A, Liao, H-X, Kepler, TB, Moody, MA, Gao, F, Danishefsky, SJ, Mascola, JR, Shaw, GM, Hahn, BH, Harrison, SC, Korber, BT, and Haynes, BF. "Staged induction of HIV-1 glycan-dependent broadly neutralizing antibodies." Science Translational Medicine 9, no. 381 (March 2017).

PMID
28298420
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Alam, SM, Aussedat, B, Vohra, Y, Meyerhoff, RR, Cale, EM, Walkowicz, WE, Radakovich, NA, Anasti, K, Armand, L, Parks, R, Sutherland, L, Scearce, R, Joyce, MG, Pancera, M, Druz, A, Georgiev, IS, Von Holle, T, Eaton, A, Fox, C, Reed, SG, Louder, M, Bailer, RT, Morris, L, Abdool-Karim, SS, Cohen, M, Liao, H-X, Montefiori, DC, Park, PK, Fernández-Tejada, A, Wiehe, K, Santra, S, Kepler, TB, Saunders, KO, Sodroski, J, Kwong, PD, Mascola, JR, Bonsignori, M, Moody, MA, Danishefsky, S, and Haynes, BF. "Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide." Science Translational Medicine 9, no. 381 (March 2017).

PMID
28298421
Full Text

Easterhoff, D, Moody, MA, Fera, D, Cheng, H, Ackerman, M, Wiehe, K, Saunders, KO, Pollara, J, Vandergrift, N, Parks, R, Kim, J, Michael, NL, O'Connell, RJ, Excler, J-L, Robb, ML, Vasan, S, Rerks-Ngarm, S, Kaewkungwal, J, Pitisuttithum, P, Nitayaphan, S, Sinangil, F, Tartaglia, J, Phogat, S, Kepler, TB, Alam, SM, Liao, H-X, Ferrari, G, Seaman, MS, Montefiori, DC, Tomaras, GD, Harrison, SC, and Haynes, BF. "Boosting of HIV envelope CD4 binding site antibodies with long variable heavy third complementarity determining region in the randomized double blind RV305 HIV-1 vaccine trial." Plos Pathogens 13, no. 2 (February 24, 2017): e1006182-null.

PMID
28235027
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Wiehe, K, Nicely, NI, Lockwood, B, Kuraoka, M, Anasti, K, Arora, S, Bowman, CM, Stolarchuk, C, Parks, R, Lloyd, KE, Xia, S-M, Duffy, R, Shen, X, Kyratsous, CA, Macdonald, LE, Murphy, AJ, Scearce, RM, Moody, MA, Alam, SM, Verkoczy, L, Tomaras, GD, Kelsoe, G, and Haynes, BF. "Immunodominance of Antibody Recognition of the HIV Envelope V2 Region in Ig-Humanized Mice." Journal of Immunology (Baltimore, Md. : 1950) 198, no. 3 (February 2017): 1047-1055.

PMID
28011932
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Saunders, KO, Nicely, NI, Wiehe, K, Bonsignori, M, Meyerhoff, RR, Parks, R, Walkowicz, WE, Aussedat, B, Wu, NR, Cai, F, Vohra, Y, Park, PK, Eaton, A, Go, EP, Sutherland, LL, Scearce, RM, Barouch, DH, Zhang, R, Von Holle, T, Overman, RG, Anasti, K, Sanders, RW, Moody, MA, Kepler, TB, Korber, B, Desaire, H, Santra, S, Letvin, NL, Nabel, GJ, Montefiori, DC, Tomaras, GD, Liao, H-X, Alam, SM, Danishefsky, SJ, and Haynes, BF. "Vaccine Elicitation of High Mannose-Dependent Neutralizing Antibodies against the V3-Glycan Broadly Neutralizing Epitope in Nonhuman Primates." Cell Reports 18, no. 9 (February 2017): 2175-2188.

PMID
28249163
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