John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Vasquez, A, Zavasky, D, Chow, NA, Gade, L, Zlatanic, E, Elkind, S, Litvintseva, AP, Pappas, PG, Perfect, JR, Revankar, S, Lockhart, SR, Chiller, T, Ackelsberg, J, and Vallabhaneni, S. "Management of an Outbreak of Exophiala dermatitidis Bloodstream Infections at an Outpatient Oncology Clinic." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 66, no. 6 (March 2018): 959-962.

PMID
29121235
Full Text

Saelens, JW, Lau-Bonilla, D, Moller, A, Xet-Mull, AM, Medina, N, Guzmán, B, Calderón, M, Herrera, R, Stout, JE, Arathoon, E, Samayoa, B, and Tobin, DM. "Annotated Genome Sequences of 16 Lineage 4 Mycobacterium tuberculosis Strains from Guatemala." Genome announcements 6, no. 7 (February 15, 2018).

PMID
29449380
Full Text

Charalambous, LT, Premji, A, Tybout, C, Hunt, A, Cutshaw, D, Elsamadicy, AA, Yang, S, Xie, J, Giamberardino, C, Pagadala, P, Perfect, JR, and Lad, SP. "Prevalence, healthcare resource utilization and overall burden of fungal meningitis in the United States." Journal of medical microbiology 67, no. 2 (February 2018): 215-227.

PMID
29244019
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Mourad, A, and Perfect, JR. "Tolerability profile of the current antifungal armoury." The Journal of antimicrobial chemotherapy 73, no. suppl_1 (January 2018): i26-i32.

PMID
29304209
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Wilkinson, RJ, Rohlwink, U, Misra, UK, van Crevel, R, Mai, NTH, Dooley, KE, Caws, M, Figaji, A, Savic, R, Solomons, R, and Thwaites, GE. "Tuberculous meningitis." Nature Reviews Neurology 13, no. 10 (September 8, 2017): 581-598.

Full Text

Arif, S, and Perfect, JR. "Emergence of the Molds Other than Aspergillus in Immunocompromised Patients." Clinics in chest medicine 38, no. 3 (September 2017): 555-573. (Review)

PMID
28797495
Full Text

Rhodes, J, Desjardins, CA, Sykes, SM, Beale, MA, Vanhove, M, Sakthikumar, S, Chen, Y, Gujja, S, Saif, S, Chowdhary, A, Lawson, DJ, Ponzio, V, Colombo, AL, Meyer, W, Engelthaler, DM, Hagen, F, Illnait-Zaragozi, MT, Alanio, A, Vreulink, J-M, Heitman, J, Perfect, JR, Litvintseva, AP, Bicanic, T, Harrison, TS, Fisher, MC, and Cuomo, CA. "Tracing Genetic Exchange and Biogeography of Cryptococcus neoformans var. grubii at the Global Population Level." Genetics 207, no. 1 (September 2017): 327-346.

PMID
28679543
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Alvarez, MI, Glover, LC, Luo, P, Wang, L, Theusch, E, Oehlers, SH, Walton, EM, Tram, TTB, Kuang, Y-L, Rotter, JI, McClean, CM, Chinh, NT, Medina, MW, Tobin, DM, Dunstan, SJ, and Ko, DC. "Human genetic variation in VAC14 regulates Salmonella invasion and typhoid fever through modulation of cholesterol." Proceedings of the National Academy of Sciences of the United States of America 114, no. 37 (September 2017): E7746-E7755.

PMID
28827342
Full Text

Perfect, JR. "The antifungal pipeline: a reality check." Nature reviews. Drug discovery 16, no. 9 (September 2017): 603-616. (Review)

PMID
28496146
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McCarthy, MW, Kontoyiannis, DP, Cornely, OA, Perfect, JR, and Walsh, TJ. "Novel Agents and Drug Targets to Meet the Challenges of Resistant Fungi." The Journal of infectious diseases 216, no. suppl_3 (August 2017): S474-S483. (Review)

PMID
28911042
Full Text

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