Alem W Kahsai, PhD

Assistant Professor in Medicine
Email address ak159@duke.edu

Education and Training

  • Ph.D., University of Connecticut, 2008

Publications

Liu, X, Ahn, S, Kahsai, AW, Meng, K-C, Latorraca, NR, Pani, B, Venkatakrishnan, AJ, Masoudi, A, Weis, WI, Dror, RO, Chen, X, Lefkowitz, RJ, and Kobilka, BK. "Mechanism of intracellular allosteric β2AR antagonist revealed by X-ray crystal structure." Nature 548, no. 7668 (August 16, 2017): 480-484.

PMID
28813418
Full Text

Dong, J-H, Wang, Y-J, Cui, M, Wang, X-J, Zheng, W-S, Ma, M-L, Yang, F, He, D-F, Hu, Q-X, Zhang, D-L, Ning, S-L, Liu, C-H, Wang, C, Wang, Y, Li, X-Y, Yi, F, Lin, A, Kahsai, AW, Cahill, TJ, Chen, Z-Y, Yu, X, and Sun, J-P. "Adaptive Activation of a Stress Response Pathway Improves Learning and Memory Through Gs and β-Arrestin-1-Regulated Lactate Metabolism." Biological psychiatry 81, no. 8 (April 2017): 654-670.

PMID
27916196
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Cahill, TJ, Thomsen, ARB, Tarrasch, JT, Plouffe, B, Nguyen, AH, Yang, F, Huang, L-Y, Kahsai, AW, Bassoni, DL, Gavino, BJ, Lamerdin, JE, Triest, S, Shukla, AK, Berger, B, Little, J, Antar, A, Blanc, A, Qu, C-X, Chen, X, Kawakami, K, Inoue, A, Aoki, J, Steyaert, J, Sun, J-P, Bouvier, M, Skiniotis, G, and Lefkowitz, RJ. "Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis." Proceedings of the National Academy of Sciences of the United States of America 114, no. 10 (March 2017): 2562-2567.

PMID
28223524
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Liu, C-H, Gong, Z, Liang, Z-L, Liu, Z-X, Yang, F, Sun, Y-J, Ma, M-L, Wang, Y-J, Ji, C-R, Wang, Y-H, Wang, M-J, Cui, F-A, Lin, A, Zheng, W-S, He, D-F, Qu, C-X, Xiao, P, Liu, C-Y, Thomsen, ARB, Joseph Cahill, T, Kahsai, AW, Yi, F, Xiao, K-H, Xue, T, Zhou, Z, Yu, X, and Sun, J-P. "Arrestin-biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling." Nature communications 8 (February 9, 2017): 14335-.

PMID
28181498
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Ahn, S, Kahsai, AW, Pani, B, Wang, Q-T, Zhao, S, Wall, AL, Strachan, RT, Staus, DP, Wingler, LM, Sun, LD, Sinnaeve, J, Choi, M, Cho, T, Xu, TT, Hansen, GM, Burnett, MB, Lamerdin, JE, Bassoni, DL, Gavino, BJ, Husemoen, G, Olsen, EK, Franch, T, Costanzi, S, Chen, X, and Lefkowitz, RJ. "Allosteric "beta-blocker" isolated from a DNA-encoded small molecule library." Proceedings of the National Academy of Sciences of the United States of America 114, no. 7 (February 2017): 1708-1713.

PMID
28130548
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Kahsai, AW, Wisler, JW, Lee, J, Ahn, S, Cahill Iii, TJ, Dennison, SM, Staus, DP, Thomsen, ARB, Anasti, KM, Pani, B, Wingler, LM, Desai, H, Bompiani, KM, Strachan, RT, Qin, X, Alam, SM, Sullenger, BA, and Lefkowitz, RJ. "Conformationally selective RNA aptamers allosterically modulate the β2-adrenoceptor." Nature chemical biology 12, no. 9 (September 2016): 709-716.

PMID
27398998
Full Text

Thomsen, ARB, Plouffe, B, Cahill, TJ, Shukla, AK, Tarrasch, JT, Dosey, AM, Kahsai, AW, Strachan, RT, Pani, B, Mahoney, JP, Huang, L, Breton, B, Heydenreich, FM, Sunahara, RK, Skiniotis, G, Bouvier, M, and Lefkowitz, RJ. "GPCR-G Protein-β-Arrestin Super-Complex Mediates Sustained G Protein Signaling." Cell 166, no. 4 (August 3, 2016): 907-919.

PMID
27499021
Full Text

Staus, DP, Strachan, RT, Manglik, A, Pani, B, Kahsai, AW, Kim, TH, Wingler, LM, Ahn, S, Chatterjee, A, Masoudi, A, Kruse, AC, Pardon, E, Steyaert, J, Weis, WI, Prosser, RS, Kobilka, BK, Costa, T, and Lefkowitz, RJ. "Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation." Nature 535, no. 7612 (July 13, 2016): 448-452.

PMID
27409812
Full Text

Shukla, AK, Westfield, GH, Xiao, K, Reis, RI, Huang, L-Y, Tripathi-Shukla, P, Qian, J, Li, S, Blanc, A, Oleskie, AN, Dosey, AM, Su, M, Liang, C-R, Gu, L-L, Shan, J-M, Chen, X, Hanna, R, Choi, M, Yao, XJ, Klink, BU, Kahsai, AW, Sidhu, SS, Koide, S, Penczek, PA, Kossiakoff, AA, Woods, VL, Kobilka, BK, Skiniotis, G, and Lefkowitz, RJ. "Visualization of arrestin recruitment by a G-protein-coupled receptor." Nature 512, no. 7513 (August 2014): 218-222.

PMID
25043026
Full Text

Kahsai, AW, Rajagopal, S, Sun, J, and Xiao, K. "Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry." Nature protocols 9, no. 6 (January 2014): 1301-1319.

PMID
24810039
Full Text

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