Bryan R. Cullen, PhD

Professor of Molecular Genetics and Microbiology
James B. Duke Professor of Molecular Genetics and Microbiology
Director, Center for Virology
Professor in Medicine
Member of the Duke Cancer Institute
Campus mail 0424 Clin & Res Labs, Durham, NC 27710
Phone (919) 684-3369
Email address bryan.cullen@duke.edu

My laboratory has for sometime been interested in understanding the molecular biology of the replication cycle of the pathogenic retrovirus HIV-1. Because HIV-1 gene expression is primarily regulated by specific RNA:protein interactions, my laboratory has also become interested in the more general area of RNA sequence mediated gene regulation, including nuclear mRNA export and the phenomenon of RNA interference.

In the past, my laboratory has worked extensively on Tat, the transcriptional regulator encoded by HIV-1, and on Rev, a virally encoded nuclear mRNA export factor. Our major focus at present is a third HIV-1 regulatory protein termed Vif. In the absence of Vif, HIV-1 virions are produced normally but are largely non-infectious. It has now been demonstrated that Vif functions to block an innate human antiretroviral defense pathway that relies on a factor called APOBEC3G or CEM15. In the absence of Vif, APOBEC3G is packaged into virions and induces degradation of the HIV-1 genome during reverse transcription in target cells. Vif directly interacts with APOBEC3G and thereby allows reverse transcription to proceed unimpeded. Among other issues, we are currently interested in how APOBEC3G is packaged into virions and in how Vif blocks APOBEC3G function. The role of APOBEC3G in cellular defense against other retroviruses and retrotransposons is also an area of interest.

A second major research area in my group relates to how microRNA precursors are processed to yield mature microRNAs and how microRNAs, and the closely related small interfering RNAs, function in human cells. We were the first group to demonstrate overexpression of human microRNAs and therefore have a system in place which should allow us to make rapid progress in this area. We also remain interested in using RNA interference to determine the role of specific cellular factors in different processes, including HIV-1 replication and nuclear mRNA export.

Education and Training

  • Ph.D., University of Medicine and Dentistry of New Jersey, 1984

Publications

Gottwein, E, Corcoran, DL, Mukherjee, N, Skalsky, RL, Hafner, M, Nusbaum, JD, Shamulailatpam, P, Love, CL, Dave, SS, Tuschl, T, Ohler, U, and Cullen, BR. "Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines." Cell Host & Microbe 10, no. 5 (November 2011): 515-526.

PMID
22100165
Full Text

Feederle, R, Haar, J, Bernhardt, K, Linnstaedt, SD, Bannert, H, Lips, H, Cullen, BR, and Delecluse, H-J. "The members of an Epstein-Barr virus microRNA cluster cooperate to transform B lymphocytes." Journal of Virology 85, no. 19 (October 2011): 9801-9810.

PMID
21752900
Full Text

Cullen, BR. "Viruses and microRNAs: RISCy interactions with serious consequences." Genes & Development 25, no. 18 (September 6, 2011): 1881-1894. (Review)

PMID
21896651
Full Text

Cullen, BR. "Herpesvirus microRNAs: phenotypes and functions." Curr Opin Virol 1, no. 3 (September 2011): 211-215. (Review)

PMID
21927637
Full Text

Cullen, BR. "Viruses and microRNAs." IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47 (June 2011): S2-S2.

Scholars@Duke

Feederle, R, Linnstaedt, SD, Bannert, H, Lips, H, Bencun, M, Cullen, BR, and Delecluse, H-J. "A viral microRNA cluster strongly potentiates the transforming properties of a human herpesvirus." Plos Pathogens 7, no. 2 (February 17, 2011): e1001294-null.

PMID
21379335
Full Text

Bogerd, HP, Zhang, F, Bieniasz, PD, and Cullen, BR. "Human APOBEC3 proteins can inhibit xenotropic murine leukemia virus-related virus infectivity." Virology 410, no. 1 (February 2011): 234-239.

PMID
21131013
Full Text

Skalsky, RL, and Cullen, BR. "Reduced expression of brain-enriched microRNAs in glioblastomas permits targeted regulation of a cell death gene." Plos One 6, no. 9 (January 2011): e24248-null.

PMID
21912681
Full Text

Linnstaedt, SD, Gottwein, E, Skalsky, RL, Luftig, MA, and Cullen, BR. "Virally induced cellular microRNA miR-155 plays a key role in B-cell immortalization by Epstein-Barr virus." Journal of Virology 84, no. 22 (November 2010): 11670-11678.

PMID
20844043
Full Text

Umbach, JL, Yen, H-L, Poon, LLM, and Cullen, BR. "Influenza A virus expresses high levels of an unusual class of small viral leader RNAs in infected cells." Mbio 1, no. 4 (September 14, 2010).

PMID
20842206
Full Text

Pages