Christopher Lee Holley, MD, PhD

Associate Professor of Medicine
Assistant Research Professor of Molecular Genetics and Microbiology
Campus mail 203 Research Drive, Msrb1 Rm 321, Durham, NC 27710
Phone (919) 668-2688

The Holley Laboratory is focused on the role of non-coding RNA (ncRNA) in cardiovascular health and disease, with a special emphasis on snoRNA (small nucleolar RNA).  snoRNAs are canonically known to guide the chemical modification of other RNAs, with ribosomal RNA being the primary target.  Dr. Holley’s research has helped to uncover a novel biologic role for the Rpl13a snoRNAs in the regulation of reactive oxygen species (ROS) and oxidative stress.  These four snoRNAs (U32a, U33, U34, and U35a) have a critical role in the oxidative stress response to a variety of stimuli, including saturated fatty acids, lipopolysaccharide, doxorubicin, and hydrogen peroxide.  The role of these snoRNAs in cardiovascular diseases such as hypertension, aortic stenosis, and heart failure is an active area of research.

Most recently, Dr. Holley’s work has shown that snoRNAs are themselves regulated by ROS and oxidative stress, with dynamic accumulation of snoRNAs in the cytoplasm in response to oxidative stress.  This raises the possibility that snoRNAs, which were thought to be exclusively nuclear, may also have cytoplasmic targets that mediate their novel biologic roles.

Education and Training

  • Cardiovascular Research Fellowship, Washington University School of Medicine, 2009 - 2012
  • Chief Fellow, Cardiovascular Division, Washington University School of Medicine, 2009 - 2010
  • Chief Resident, Internal Medicine, Washington University School of Medicine, 2007 - 2008
  • Cardiovascular Clinical Fellowship, Washington University School of Medicine, 2006 - 2009
  • Internal Medicine Residency, Washington University School of Medicine, 2004 - 2006
  • M.D., Duke University School of Medicine, 2004
  • Ph.D., Duke University School of Medicine, 2003


Truby, Lauren K., Sarah Casalinova, Chetan B. Patel, Richa Agarwal, Christopher L. Holley, Robert J. Mentz, Carmelo Milano, Benjamin Bryner, Jacob N. Schroder, and Adam D. DeVore. “Donation After Circulatory Death in Heart Transplantation: History, Outcomes, Clinical Challenges, and Opportunities to Expand the Donor Pool.” J Card Fail, April 18, 2022.

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Paolillo, Roberta, Giacomo Gabriele Schiattarella, Stefania D’Apice, Christopher Holley, Alessandro Della Corte, Ciro Bancone, Cinzia Perrino, and Giovanni Esposito. “183 Small nucleolar RNA SNORD3A: a potential new biomarker and molecular player in heart failure.” In European Heart Journal Supplements, Vol. 23. Oxford University Press (OUP), 2021.

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Truby, Lauren K., Lydia Coulter Kwee, Richa Agarwal, Elizabeth Grass, Adam D. DeVore, Chetan B. Patel, Dongfeng Chen, et al. “Proteomic profiling identifies CLEC4C expression as a novel biomarker of primary graft dysfunction after heart transplantation.” J Heart Lung Transplant 40, no. 12 (December 2021): 1589–98.

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Martinez Campos, Cecilia, Kevin Tsai, David G. Courtney, Hal P. Bogerd, Christopher L. Holley, and Bryan R. Cullen. “Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique.” Rna 27, no. 11 (November 2021): 1400–1411.

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Paolillo, R., S. D’Apice, G. G. Schiattarella, C. L. Holley, A. Della Corte, C. L. Bancone, G. G. Esposito, and C. L. Perrino. “Small nucleolar RNA SNORD3A: a potential new biomarker and molecular player in heart failure.” In European Heart Journal, 42:3281–3281, 2021.


Truby, L. K., L. C. Kwee, R. Agarwal, E. Grass, A. D. Devore, C. B. Patel, G. Rogers, et al. “Proteomic Profiling of Heart Transplant Recipients Identifies CLE4C Expression as a Novel Biomarker of Primary Graft Dysfunction.” In Journal of Heart and Lung Transplantation, 40:S49–S49, 2021.


Chao, Yvonne, Yinzhou Zhu, Hannah J. Wiedner, Yi-Hsuan Tsai, Lily Wilkinson, Alessandro Porrello, Amanda E. D. Van Swearingen, et al. “Snord67 promotes lymph node metastasis through U6-mediated alternative splicing in breast cancer.” In Cancer Research, Vol. 81, 2021.


Abou Assi, Hala, Atul K. Rangadurai, Honglue Shi, Bei Liu, Mary C. Clay, Kevin Erharter, Christoph Kreutz, Christopher L. Holley, and Hashim M. Al-Hashimi. “2'-O-Methylation can increase the abundance and lifetime of alternative RNA conformational states.” Nucleic Acids Res 48, no. 21 (December 2, 2020): 12365–79.

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