John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division Of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Abene, MV, and Hamilton, JD. "Resolution of fear of flying with fluoxetine treatment." J Anxiety Disord 12, no. 6 (November 1998): 599-603.

PMID
9879038
Scholars@Duke

Hamilton, JD, and Workman, RH. "Persistence of combat-related posttraumatic stress symptoms for 75 years." J Trauma Stress 11, no. 4 (October 1998): 763-768.

PMID
9870226
Full Text

Schmader, K, George, LK, Burchett, BM, Hamilton, JD, and Pieper, CF. "Race and stress in the incidence of herpes zoster in older adults." J Am Geriatr Soc 46, no. 8 (August 1998): 973-977.

PMID
9706885
Scholars@Duke

Hamilton, JD. "A deanship at Newcastle." Med J Aust 168, no. 1 (January 5, 1998): 34-37.

PMID
9451394
Scholars@Duke

O'Brien, WA, Hartigan, PM, Daar, ES, Simberkoff, MS, and Hamilton, JD. "Changes in plasma HIV RNA levels and CD4+ lymphocyte counts predict both response to antiretroviral therapy and therapeutic failure. VA Cooperative Study Group on AIDS." Annals of Internal Medicine 126, no. 12 (June 1997): 939-945.

PMID
9182470
Full Text

Bakey, AA, Kunik, ME, Orengo, CA, Molinari, VA, Workman, RH, and Hamilton, JD. "Outcome of psychiatric hospitalization for very low-functioning demented patients." Journal of Geriatric Psychiatry and Neurology 10, no. 2 (April 1997): 55-57.

PMID
9188019
Full Text

Ponce, H, Kunik, ME, Molinari, V, and Hamilton, JD. "Why elderly veterans choose VA services." Psychiatr Serv 48, no. 1 (January 1997): 106-107. (Letter)

PMID
9117491
Full Text

Sharara, AI, Hunt, CM, and Hamilton, JD. "Hepatitis C." Ann Intern Med 125, no. 8 (October 15, 1996): 658-668. (Review)

PMID
8849151
Scholars@Duke

Hamilton, JD, Freeman, MB, and Reinert, KH. "Aquatic risk assessment of a polycarboxylate dispersant polymer used in laundry detergents." J Toxicol Environ Health 49, no. 1 (September 1996): 67-82. (Review)

PMID
8816934
Scholars@Duke

Wilson, RM, Runciman, WB, Gibberd, RW, Harrison, BT, and Hamilton, JD. "Quality in Australian Health Care Study." Med J Aust 164, no. 12 (June 17, 1996): 754-. (Letter)

PMID
8668084
Scholars@Duke

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