John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Webb, J. P., J. D. Hamilton, and A. M. Dawson. “A physicochemical study of fat absorption in rats. Limitation of methods in vitro..” Biochim Biophys Acta 187, no. 1 (July 29, 1969): 42–52. https://doi.org/10.1016/0005-2760(69)90131-3.

PMID
5811213
Full Text

Hamilton, J. D., J. P. Webb, and A. M. Dawson. “The absorption of tristearin and stearic acid and tripalmitin and palmitic acid. Studies on the rate-limiting steps in rats..” Biochim Biophys Acta 176, no. 1 (January 21, 1969): 27–36. https://doi.org/10.1016/0005-2760(69)90069-1.

PMID
5766024
Full Text

Hamilton, J. D., and H. B. McMichael. “Role of the microvillus in the absorption of disaccharides..” Lancet 2, no. 7560 (July 20, 1968): 154–57. https://doi.org/10.1016/s0140-6736(68)90429-7.

PMID
4173271
Full Text

Hamilton, J. D., A. M. Dawson, and P. W. Webb. “Observations upon small gut "mucosal" pO2 and pCO2 in anesthetized dogs..” Gastroenterology 55, no. 1 (July 1968): 52–60.

PMID
5663505
Scholars@Duke

Hamilton, J. D., A. M. Dawson, and J. Webb. “Limitation of the use of inert gases in the measurement of small gut mucosal blood flow..” Gut 8, no. 5 (October 1967): 509–21. https://doi.org/10.1136/gut.8.5.509.

PMID
6057782
Full Text

GROSS, N. J., and J. D. HAMILTON. “CORRELATION BETWEEN THE PHYSICAL SIGNS OF HYPERCAPNIA AND THE MIXED VENOUS PCO2..” Br Med J 2, no. 5365 (November 2, 1963): 1096–97. https://doi.org/10.1136/bmj.2.5365.1096.

PMID
14057711
Full Text

HAMILTON, J. D. “The pathology of primary biliary cirrhosis..” Lab Invest 8, no. 3 (May 1959): 701–22.

PMID
13655597
Scholars@Duke

HAMILTON, J. D. “[Coronary disease]..” Strasb Med 9, no. 11 (December 1958): 905–6.

PMID
13624929
Scholars@Duke

Pages