John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

HAMILTON, J. D. “Tissue lesions in hypersensitivity..” Can Med Assoc J 78, no. 11 (June 1, 1958): 834–39.

PMID
13536937
Scholars@Duke

HAMILTON, J. D., A. SEPP, T. C. BROWN, and F. W. MACDONALD. “Morphological changes in smokers' lungs..” Can Med Assoc J 77, no. 3 (August 1, 1957): 177–82.

PMID
13446794
Scholars@Duke

WRENSHALL, G. A., and J. D. HAMILTON. “Sex differences in the amount of insulin extractable from diabetic human pancreas..” Ann N Y Acad Sci 71, no. 1 (July 10, 1957): 154–63.

PMID
13459212
Scholars@Duke

BAKER, S. M., and J. D. HAMILTON. “Capillary changes in myxedema..” Lab Invest 6, no. 3 (May 1957): 218–26.

PMID
13429842
Scholars@Duke

HAMILTON, J. D., and J. H. MOWBRAY. “The manner of death in coronary artery disease..” Can Med Assoc J 76, no. 1 (January 1, 1957): 9–15.

PMID
13374655
Scholars@Duke

HAMILTON, J. D. “Coronary artery disease..” J Ark Med Soc 52, no. 1 (June 1955): 4–10.

PMID
14381353
Scholars@Duke

ROY, T. E., J. D. HAMILTON, and L. GREENBERG. “Wound contamination and wound infection..” J R Army Med Corps 100, no. 4 (October 1954): 276–95.

PMID
13212785
Scholars@Duke

HAMILTON, J. D., and N. E. FREMES. “The natural history of experimental glomerulonephritis produced by foreign protein..” Am J Pathol 30, no. 1 (January 1954): 127–45.

PMID
13124463
Scholars@Duke

Pages