John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Kanj, S. S., A. I. Sharara, P. A. Clavien, and J. D. Hamilton. “Cytomegalovirus infection following liver transplantation: review of the literature..” Clinical Infectious Diseases : An Official Publication of the Infectious Diseases Society of America 22, no. 3 (March 1996): 537–49. https://doi.org/10.1093/clinids/22.3.537.

PMID
8852975
Full Text

Simberkoff, M. S., P. M. Hartigan, J. D. Hamilton, P. L. Day, G. R. Diamond, G. M. Dickinson, G. L. Drusano, et al. “Long-term follow-up of symptomatic HIV-infected patients originally randomized to early versus later zidovudine treatment; report of a Veterans Affairs Cooperative Study. VA Cooperative Study Group on AIDS Treatment..” Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association 11, no. 2 (February 1996): 142–50. https://doi.org/10.1097/00042560-199602010-00005.

PMID
8556396
Full Text

O’Brien, W. A., P. M. Hartigan, D. Martin, J. Esinhart, A. Hill, S. Benoit, M. Rubin, M. S. Simberkoff, and J. D. Hamilton. “Changes in plasma HIV-1 RNA and CD4+ lymphocyte counts and the risk of progression to AIDS. Veterans Affairs Cooperative Study Group on AIDS..” The New England Journal of Medicine 334, no. 7 (February 1996): 426–31. https://doi.org/10.1056/nejm199602153340703.

PMID
8552144
Full Text

Ignatowski, A. J., J. D. Hamilton, and E. D. Weiler. “Review of international criteria and mixture rules for health hazard classification..” Regulatory Toxicology and Pharmacology : Rtp 22, no. 3 (December 1995): 231–42. https://doi.org/10.1006/rtph.1995.0005.

PMID
8837847
Full Text

Wilson, R. M., W. B. Runciman, R. W. Gibberd, B. T. Harrison, L. Newby, and J. D. Hamilton. “The Quality in Australian Health Care Study..” The Medical Journal of Australia 163, no. 9 (November 1995): 458–71.

PMID
7476634
Scholars@Duke

Schmader, K., S. C. Henry, R. J. Rahija, Y. Yu, G. G. Daley, and J. D. Hamilton. “Mouse cytomegalovirus reactivation in severe combined immune deficient mice after implantation of latently infected salivary gland..” The Journal of Infectious Diseases 172, no. 2 (August 1995): 531–34. https://doi.org/10.1093/infdis/172.2.531.

PMID
7622898
Full Text

Yu, Y., S. C. Henry, F. Xu, and J. D. Hamilton. “Expression of a murine cytomegalovirus early-late protein in "latently" infected mice..” The Journal of Infectious Diseases 172, no. 2 (August 1995): 371–79. https://doi.org/10.1093/infdis/172.2.371.

PMID
7622880
Full Text

Hamilton, J. D., and E. J. O’Flaherty. “Influence of lead on mineralization during bone growth..” Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology 26, no. 2 (July 1995): 265–71. https://doi.org/10.1006/faat.1995.1097.

PMID
7589915
Full Text

Walters, W. A., V. Sivanesaratnam, and J. D. Hamilton. “External examiners..” Lancet (London, England) 345, no. 8957 (April 1995): 1093–95. https://doi.org/10.1016/s0140-6736(95)90823-4.

PMID
7715344
Full Text

Pages