John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division Of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Kanj, SS, Sharara, AI, Clavien, PA, and Hamilton, JD. "Cytomegalovirus infection following liver transplantation: review of the literature." Clin Infect Dis 22, no. 3 (March 1996): 537-549. (Review)

PMID
8852975
Scholars@Duke

Simberkoff, MS, Hartigan, PM, Hamilton, JD, Day, PL, Diamond, GR, Dickinson, GM, Drusano, GL, Egorin, MJ, George, WL, Gordin, FM, Hawkes, CA, Jensen, PC, Kilmas, NG, Labriola, AM, O'Brien, WA, Oster, CN, Weinhold, KJ, Wray, NP, and Pazner, SB. "Long-term follow-up of symptomatic HIV-infected patients originally randomized to early versus later zidovudine treatment; report of a Veterans Affairs Cooperative Study. VA Cooperative Study Group on AIDS Treatment." J Acquir Immune Defic Syndr Hum Retrovirol 11, no. 2 (February 1, 1996): 142-150.

PMID
8556396
Scholars@Duke

O'Brien, WA, Hartigan, PM, Martin, D, Esinhart, J, Hill, A, Benoit, S, Rubin, M, Simberkoff, MS, and Hamilton, JD. "Changes in plasma HIV-1 RNA and CD4+ lymphocyte counts and the risk of progression to AIDS. Veterans Affairs Cooperative Study Group on AIDS." The New England Journal of Medicine 334, no. 7 (February 1996): 426-431.

PMID
8552144
Full Text

Ignatowski, AJ, Hamilton, JD, and Weiler, ED. "Review of international criteria and mixture rules for health hazard classification." Regul Toxicol Pharmacol 22, no. 3 (December 1995): 231-242.

PMID
8837847
Full Text

Wilson, RM, Runciman, WB, Gibberd, RW, Harrison, BT, Newby, L, and Hamilton, JD. "The Quality in Australian Health Care Study." Med J Aust 163, no. 9 (November 6, 1995): 458-471.

PMID
7476634
Scholars@Duke

Schmader, K, Henry, SC, Rahija, RJ, Yu, Y, Daley, GG, and Hamilton, JD. "Mouse cytomegalovirus reactivation in severe combined immune deficient mice after implantation of latently infected salivary gland." J Infect Dis 172, no. 2 (August 1995): 531-534.

PMID
7622898
Scholars@Duke

Yu, Y, Henry, SC, Xu, F, and Hamilton, JD. "Expression of a murine cytomegalovirus early-late protein in "latently" infected mice." J Infect Dis 172, no. 2 (August 1995): 371-379.

PMID
7622880
Scholars@Duke

Hamilton, JD, and O'Flaherty, EJ. "Influence of lead on mineralization during bone growth." Fundam Appl Toxicol 26, no. 2 (July 1995): 265-271.

PMID
7589915
Scholars@Duke

Hamilton, JD. "Establishing standards and measurement methods for medical education." Acad Med 70, no. 7 Suppl (July 1995): S51-S56.

PMID
7626160
Scholars@Duke

Walters, WA, Sivanesaratnam, V, and Hamilton, JD. "External examiners." Lancet 345, no. 8957 (April 29, 1995): 1093-1095.

PMID
7715344
Scholars@Duke

Pages