John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Schmader, K., L. K. George, B. M. Burchett, C. F. Pieper, and J. D. Hamilton. “Racial differences in the occurrence of herpes zoster..” J Infect Dis 171, no. 3 (March 1995): 701–4. https://doi.org/10.1093/infdis/171.3.701.

PMID
7876622
Full Text

Schmader, K., L. K. George, R. Newton, and J. D. Hamilton. “The accuracy of self-report of herpes zoster..” J Clin Epidemiol 47, no. 11 (November 1994): 1271–76. https://doi.org/10.1016/0895-4356(94)90132-5.

PMID
7722563
Full Text

Gordin, F. M., P. M. Hartigan, N. G. Klimas, S. B. Zolla-Pazner, M. S. Simberkoff, and J. D. Hamilton. “Delayed-type hypersensitivity skin tests are an independent predictor of human immunodeficiency virus disease progression. Department of Veterans Affairs Cooperative Study Group..” J Infect Dis 169, no. 4 (April 1994): 893–97. https://doi.org/10.1093/infdis/169.4.893.

PMID
7907646
Full Text

Hamilton, J. D., E. J. O’Flaherty, R. Ross, R. Shukla, and P. S. Gartside. “Structural equation modeling and nested ANOVA: effects of lead exposure on maternal and fetal growth in rats..” Environ Res 64, no. 1 (January 1994): 53–64. https://doi.org/10.1006/enrs.1994.1006.

PMID
8287842
Full Text

Hartigan, P. M., and J. D. Hamilton. “Seroprevalence and seroincidence of HIV-1 in injection drug users using the VA system..” Aids Res Hum Retroviruses 10 Suppl 2 (1994): S215–17.

PMID
7865304
Scholars@Duke

Oddone, E. Z., P. Cowper, J. D. Hamilton, D. B. Matchar, P. Hartigan, G. Samsa, M. Simberkoff, and J. R. Feussner. “Cost effectiveness analysis of early zidovudine treatment of HIV infected patients..” Bmj 307, no. 6915 (November 20, 1993): 1322–25. https://doi.org/10.1136/bmj.307.6915.1322.

PMID
8257887
Full Text

Dukes, C. S., A. C. Street, J. F. Starling, and J. D. Hamilton. “Hepatitis B vaccination and booster in predialysis patients: a 4-year analysis..” Vaccine 11, no. 12 (September 1993): 1229–32. https://doi.org/10.1016/0264-410x(93)90047-2.

PMID
8256504
Full Text

Pate, L. A., J. D. Hamilton, R. S. Park, and R. M. Strobel. “Evaluation of a saliva alcohol test stick as a therapeutic adjunct in an alcoholism treatment program..” J Stud Alcohol 54, no. 5 (September 1993): 520–21. https://doi.org/10.15288/jsa.1993.54.520.

PMID
8412140
Full Text

St Clair, M. H., P. M. Hartigan, J. C. Andrews, C. L. Vavro, M. S. Simberkoff, and J. D. Hamilton. “Zidovudine resistance, syncytium-inducing phenotype, and HIV disease progression in a case-control study. The VA Cooperative Study Group..” J Acquir Immune Defic Syndr 6, no. 8 (August 1993): 891–97.

PMID
8315574
Scholars@Duke

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