John David Hamilton, MD

Professor Emeritus of Medicine
Campus mail Division of Infectious Diseases, Room 185 Hanes House, Durham, NC 27710
Phone (919) 668-3368
Email address hamil008@mc.duke.edu

1) My laboratory is engaged in studies of viral latency using murine cytomegalovirus, a member of the family of beta-Herpes viruses. Of particular interest in our laboratory, has been the mechanisms of control of latent virus. This question is, we believe, a biologically very important one since it has direct and indirect implications for viral transmission, prevention and treatment of disease.

2) In experiments using reverse transcriptase PCR, we were able to detect immediate-early I transcripts at a level roughly 10-fold higher than that of viral genome, indicating that a significant fraction of latent virus is transcriptionally active. These findings may be indicative of an abortive infection in which transcription is limited to the immediate-early genes, or the result of a very low level persistent infection, or a transient reactivation of latent virus. To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. We have further established the gene map location encoding one of these unique proteins and is found to be distinct from the immediate early genes. Further studies using immunohistochemistry indicate that this viral gene is active during latency although not exclusively and therefore not likely to be a regulatory gene for latency. Presently using a recombinant virus containing the gene for green fluorescent protein we will attempt to localize the cellular reservoir latent virus by confocal microscopy.

3) In addition, I have been very active in the conduct of clinical trials relevant to HIV/AIDS. These include a study of early versus late AZT of which I was co-principal investigator in this VA Cooperative Study and I am principal investigator for a pending application to the NIH for an Adult AIDS Clinical Trials Network.

4) I am both a frequent reviewer of manuscripts submitted for publication in the general area of basic and clinical virology and I have been appointed to several national advisory committees including White House Task Force on HIV Vaccine (FCCSET), the Medical Research Advisory Committee of the Department of Veterans Affairs, and the FDA Antiviral Advisory Committee.

5) Key Words
Virus, latency, molecular, cytomegalovirus, immunocompromised, HIV, hepatitis

Education and Training

  • M.D., University of Colorado at Colorado Springs, 1964

Publications

Hamilton, J. D., P. M. Hartigan, M. S. Simberkoff, P. L. Day, G. R. Diamond, G. M. Dickinson, G. L. Drusano, M. J. Egorin, W. L. George, and F. M. Gordin. “A controlled trial of early versus late treatment with zidovudine in symptomatic human immunodeficiency virus infection. Results of the Veterans Affairs Cooperative Study..” N Engl J Med 326, no. 7 (February 13, 1992): 437–43. https://doi.org/10.1056/NEJM199202133260703.

PMID
1346337
Full Text

Schmader, K. E., R. J. Rahija, K. R. Porter, and J. D. Hamilton. “Murine cytomegalovirus gene amplification and culture after submaxillary salivary gland biopsy..” Lab Anim Sci 41, no. 5 (October 1991): 396–400.

PMID
1666135
Scholars@Duke

Hamilton, J. D., and O. Ogunbode. “Medical education in the community: a Nigerian experience..” Lancet 338, no. 8759 (July 13, 1991): 99–102. https://doi.org/10.1016/0140-6736(91)90087-6.

PMID
1676480
Full Text

Simberkoff, M. S., P. M. Hartigan, and J. D. Hamilton. “Ethical dilemma in continuing a zidovudine-placebo trial in symptomatic human immunodeficiency virus infection..” Trans Assoc Am Physicians 104 (1991): 92–96.

PMID
1845159
Scholars@Duke

Hamilton, J. D., and J. M. Vandewerdt. “The accreditation of undergraduate medical education in Australia..” Med J Aust 153, no. 9 (November 5, 1990): 541–45.

PMID
2233477
Scholars@Duke

Hamilton, J. D., M. S. Simberkoff, and P. Hartigan. “Treatment of mildly symptomatic human immunodeficiency virus (HIV) infection..” Ann Intern Med 113, no. 8 (October 15, 1990): 635–36. https://doi.org/10.7326/0003-4819-113-8-635.

PMID
2400172
Full Text

Street, A. C., T. Z. Weddle, W. R. Thomann, E. W. Lundberg, G. W. Jackson, and J. D. Hamilton. “Persistence of antibody in healthcare workers vaccinated against hepatitis B..” Infect Control Hosp Epidemiol 11, no. 10 (October 1990): 525–30. https://doi.org/10.1086/646086.

PMID
2146315
Full Text

Shaunak, S., R. E. Albright, M. E. Klotman, S. C. Henry, J. A. Bartlett, and J. D. Hamilton. “Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease..” J Infect Dis 161, no. 6 (June 1990): 1068–72. https://doi.org/10.1093/infdis/161.6.1068.

PMID
2189004
Full Text

Klotman, M. E., S. C. Henry, R. C. Greene, P. C. Brazy, P. E. Klotman, and J. D. Hamilton. “Detection of mouse cytomegalovirus nucleic acid in latently infected mice by in vitro enzymatic amplification..” J Infect Dis 161, no. 2 (February 1990): 220–25. https://doi.org/10.1093/infdis/161.2.220.

PMID
2153738
Full Text

Pages