John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Kovanda, Laura L., Charles Giamberardino, Laura McEntee, Dena L. Toffaletti, Kelly S. Franke, Andrew Bartuska, Gordon Smilnak, et al. “Pharmacodynamics of Isavuconazole in a Rabbit Model of Cryptococcal Meningoencephalitis..” Antimicrob Agents Chemother 63, no. 9 (September 2019). https://doi.org/10.1128/AAC.00546-19.

PMID
31209006
Full Text

Hommel, Benjamin, Aude Sturny-Leclère, Stevenn Volant, Nathanaël Veluppillai, Magalie Duchateau, Chen-Hsin Yu, Véronique Hourdel, et al. “Correction: Cryptococcus neoformans resists to drastic conditions by switching to viable but non-culturable cell phenotype..” Plos Pathog 15, no. 9 (September 2019). https://doi.org/10.1371/journal.ppat.1008070.

PMID
31527911
Full Text

Jaeger, Martin, Vasiliki Matzaraki, Raúl Aguirre-Gamboa, Mark S. Gresnigt, Xiaojing Chu, Melissa D. Johnson, Marije Oosting, et al. “A Genome-Wide Functional Genomics Approach Identifies Susceptibility Pathways to Fungal Bloodstream Infection in Humans..” J Infect Dis 220, no. 5 (July 31, 2019): 862–72. https://doi.org/10.1093/infdis/jiz206.

PMID
31241743
Full Text

Hommel, Benjamin, Aude Sturny-Leclère, Stevenn Volant, Nathanaël Veluppillai, Magalie Duchateau, Chen-Hsin Yu, Véronique Hourdel, et al. “Cryptococcus neoformans resists to drastic conditions by switching to viable but non-culturable cell phenotype..” Plos Pathog 15, no. 7 (July 2019). https://doi.org/10.1371/journal.ppat.1007945.

PMID
31356623
Full Text

Nguyen, M Hong, Cornelius J. Clancy, A William Pasculle, Peter G. Pappas, George Alangaden, George A. Pankey, Bryan H. Schmitt, et al. “Performance of the T2Bacteria Panel for Diagnosing Bloodstream Infections: A Diagnostic Accuracy Study..” Ann Intern Med 170, no. 12 (June 18, 2019): 845–52. https://doi.org/10.7326/M18-2772.

PMID
31083728
Full Text

Ashton, P. M., L. T. Thanh, P. H. Trieu, D. Van Anh, N. M. Trinh, J. Beardsley, F. Kibengo, et al. “Three phylogenetic groups have driven the recent population expansion of Cryptococcus neoformans..” Nat Commun 10, no. 1 (May 2, 2019). https://doi.org/10.1038/s41467-019-10092-5.

PMID
31048698
Full Text

Sundermann, Alexander J., Cornelius J. Clancy, A William Pasculle, Guojun Liu, Richard B. Cumbie, Eileen Driscoll, Ashley Ayres, et al. “How Clean Is the Linen at My Hospital? The Mucorales on Unclean Linen Discovery Study of Large United States Transplant and Cancer Centers..” Clin Infect Dis 68, no. 5 (February 15, 2019): 850–53. https://doi.org/10.1093/cid/ciy669.

PMID
30299481
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Le, Thuy, Vo Trieu Ly, Nguyen Thi Mai Thu, Ashley Nguyen, Nguyen Tat Thanh, Nguyen Van Vinh Chau, Guy Thwaites, John Perfect, Ruwanthi Kolamunnage-Dona, and William Hope. “Population Pharmacodynamics of Amphotericin B Deoxycholate for Disseminated Infection Caused by Talaromyces marneffei..” Antimicrob Agents Chemother 63, no. 2 (February 2019). https://doi.org/10.1128/AAC.01739-18.

PMID
30420478
Full Text

Mourad, Ahmad, Jonathan A. Stiber, John R. Perfect, and Melissa D. Johnson. “Real-world implications of QT prolongation in patients receiving voriconazole and amiodarone..” J Antimicrob Chemother 74, no. 1 (January 1, 2019): 228–33. https://doi.org/10.1093/jac/dky392.

PMID
30295798
Full Text

Ponzio, Vinicius, Yuan Chen, Anderson Messias Rodrigues, Jennifer L. Tenor, Dena L. Toffaletti, José Osmar Medina-Pestana, Arnaldo Lopes Colombo, and John R. Perfect. “Genotypic diversity and clinical outcome of cryptococcosis in renal transplant recipients in Brazil..” Emerg Microbes Infect 8, no. 1 (2019): 119–29. https://doi.org/10.1080/22221751.2018.1562849.

PMID
30866766
Full Text

Pages