John Robert Perfect, MD

Professor of Medicine
James B. Duke Distinguished Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan, Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., The University of Toledo, 1974

Publications

Driemeyer, Cândida, Diego R. Falci, Martin Hoenigl, Oliver A. Cornely, Arunaloke Chakrabarti, Jean Pierre Gangneux, Esther Segal, et al. “The current state of Clinical Mycology in Eastern and South-Eastern Europe.” Med Mycol 60, no. 4 (March 22, 2022). https://doi.org/10.1093/mmy/myac017.

PMID
35195256
Full Text

Drew, Richard H., and John R. Perfect. “Conventional Antifungals for Invasive Infections Delivered by Unconventional Methods; Aerosols, Irrigants, Directed Injections and Impregnated Cement.” J Fungi (Basel) 8, no. 2 (February 21, 2022). https://doi.org/10.3390/jof8020212.

PMID
35205966
Full Text

Perfect, John R., Damian J. Krysan, Maurizio Del Poeta, Anna M. Selmecki, Jessica C. S. Brown, and Leah E. Cowen. “Editorial: Antifungal Pipeline: Build It Strong; Build It Better!.” Front Cell Infect Microbiol 12 (2022): 881272. https://doi.org/10.3389/fcimb.2022.881272.

PMID
35372097
Full Text

Wang, Yina, Maggie Wear, Gurkirat Kohli, Raghav Vij, Charles Giamberardino, Arpun Shah, Dena L. Toffaletti, et al. “Inositol Metabolism Regulates Capsule Structure and Virulence in the Human Pathogen Cryptococcus neoformans.” Mbio 12, no. 6 (December 21, 2021): e0279021. https://doi.org/10.1128/mBio.02790-21.

PMID
34724824
Full Text

Yu, Chen-Hsin, Poppy Sephton-Clark, Jennifer L. Tenor, Dena L. Toffaletti, Charles Giamberardino, Miriam Haverkamp, Christina A. Cuomo, and John R. Perfect. “Gene Expression of Diverse Cryptococcus Isolates during Infection of the Human Central Nervous System.” Mbio 12, no. 6 (December 21, 2021): e0231321. https://doi.org/10.1128/mBio.02313-21.

PMID
34724829
Full Text

Messina, Julia A., Eileen K. Maziarz, John Galgiani, Jonathan T. Truong, Aung K. Htoo, Arash Heidari, Royce H. Johnson, et al. “A randomized, double-blind, placebo-controlled clinical trial of fluconazole as early empiric treatment of coccidioidomycosis pneumonia (Valley Fever) in adults presenting with community-acquired pneumonia in endemic areas (FLEET-Valley Fever).” Contemp Clin Trials Commun 24 (December 2021): 100851. https://doi.org/10.1016/j.conctc.2021.100851.

PMID
34712863
Full Text

Chen, Sharon C-A, John Perfect, Arnaldo L. Colombo, Oliver A. Cornely, Andreas H. Groll, Danila Seidel, Kerstin Albus, et al. “Global guideline for the diagnosis and management of rare yeast infections: an initiative of the ECMM in cooperation with ISHAM and ASM.” Lancet Infect Dis 21, no. 12 (December 2021): e375–86. https://doi.org/10.1016/S1473-3099(21)00203-6.

PMID
34419208
Full Text

Johnson, Melissa D., Russell E. Lewis, Elizabeth S. Dodds Ashley, John R. Perfect, and Dimitrios P. Kontoyiannis. “Reply to Day et al.” J Infect Dis 224, no. 9 (November 16, 2021): 1627–28. https://doi.org/10.1093/infdis/jiab183.

PMID
33822103
Full Text

Narayanasamy, Shanti, Vu Quoc Dat, Nguyen Tat Thanh, Vo Trieu Ly, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Chuanyi Ning, et al. “A global call for talaromycosis to be recognised as a neglected tropical disease.” Lancet Glob Health 9, no. 11 (November 2021): e1618–22. https://doi.org/10.1016/S2214-109X(21)00350-8.

PMID
34678201
Full Text

Yang, Dong-Hoon, Matthew R. England, Helene Salvator, Seher Anjum, Yoon-Dong Park, Kieren A. Marr, Laurie A. Chu, et al. “Cryptococcus gattii Species Complex as an Opportunistic Pathogen: Underlying Medical Conditions Associated with the Infection.” Mbio 12, no. 5 (October 26, 2021): e0270821. https://doi.org/10.1128/mBio.02708-21.

PMID
34700378
Full Text

Pages