Infectious Diseases

John Robert Perfect, MD

Professor of Medicine
James B. Duke Distinguished Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan, Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., The University of Toledo, 1974

Grants

Publications

Rolfe, Robert, Wiley A. Schell, Becky Smith, Jacob Klapper, John R. Perfect, and Julia A. Messina. “Black mold takes hold and story told.” Med Mycol Case Rep 29 (September 2020): 12–14. https://doi.org/10.1016/j.mmcr.2020.05.005.

PMID
32528839
Full Text

Johnson, Melissa D., Russell E. Lewis, Elizabeth S. Dodds Ashley, Luis Ostrosky-Zeichner, Theoklis Zaoutis, George R. Thompson, David R. Andes, et al. “Core Recommendations for Antifungal Stewardship: A Statement of the Mycoses Study Group Education and Research Consortium.” J Infect Dis 222, no. Supplement_3 (August 5, 2020): S175–98. https://doi.org/10.1093/infdis/jiaa394.

PMID
32756879
Full Text

Pappas, Peter G., David R. Boulware, Dimitrios P. Kontoyiannis, Marisa H. Miceli, Luis Ostrosky-Zeichner, Andrej Spec, George R. Thompson, Sharon Chen, John R. Perfect, and John R. MSGERC investigators. “Clinical mycology today: A synopsis of the mycoses study group education and research consortium (MSGERC) second biennial meeting, September 27-30, 2018, Big Sky, Montana, a proposed global research agenda.” Med Mycol 58, no. 5 (July 1, 2020): 569–78. https://doi.org/10.1093/mmy/myaa034.

PMID
32407483
Full Text

Thu, Nguyen T. M., Jasper F. W. Chan, Vo Trieu Ly, Hoa T. Ngo, Ha T. A. Hien, Nguyen P. H. Lan, Nguyen V. V. Chau, et al. “Superiority of a novel Mp1p antigen detection enzyme immunoassay compared to standard BACTEC blood culture in the diagnosis of talaromycosis.” Clin Infect Dis, June 21, 2020. https://doi.org/10.1093/cid/ciaa826.

PMID
32564074
Full Text

Toplis, Barbra, Caylin Bosch, Ilan S. Schwartz, Chris Kenyon, Teun Boekhout, John R. Perfect, and Alfred Botha. “The virulence factor urease and its unexplored role in the metabolism of Cryptococcus neoformans.” Fems Yeast Res 20, no. 4 (June 1, 2020). https://doi.org/10.1093/femsyr/foaa031.

PMID
32490521
Full Text

Eichenberger, Emily M., Jennifer Saullo, Danielle Brander, Shih-Hsiu Wang, John R. Perfect, and Julia A. Messina. “A case of CNS aspergillosis in a patient with chronic lymphocytic leukemia on first-line ibrutinib therapy.” Med Mycol Case Rep 27 (March 2020): 17–21. https://doi.org/10.1016/j.mmcr.2019.12.007.

PMID
31879587
Full Text

Kiyani, Musa, Beiyu Liu, Lefko T. Charalambous, Syed M. Adil, Sarah E. Hodges, Siyun Yang, Promila Pagadala, John R. Perfect, and Shivanand P. Lad. “The longitudinal health economic impact of viral encephalitis in the United States.” J Med Microbiol 69, no. 2 (February 2020): 270–79. https://doi.org/10.1099/jmm.0.001153.

PMID
32040394
Full Text

Baker, Arthur W., Eileen K. Maziarz, Christopher J. Arnold, Melissa D. Johnson, Adrienne D. Workman, John M. Reynolds, John R. Perfect, and Barbara D. Alexander. “Invasive Fungal Infection After Lung Transplantation: Epidemiology in the Setting of Antifungal Prophylaxis.” Clin Infect Dis 70, no. 1 (January 1, 2020): 30–39. https://doi.org/10.1093/cid/ciz156.

PMID
30801642
Full Text

Hope, William, Neil R. H. Stone, Adam Johnson, Laura McEntee, Nicola Farrington, Anahi Santoro-Castelazo, Xuan Liu, et al. “Fluconazole Monotherapy Is a Suboptimal Option for Initial Treatment of Cryptococcal Meningitis Because of Emergence of Resistance.” Mbio 10, no. 6 (December 3, 2019). https://doi.org/10.1128/mBio.02575-19.

PMID
31796539
Full Text

So, Yee-Seul, Shinae Maeng, Dong-Hoon Yang, Hyelim Kim, Kyung-Tae Lee, Seong-Ryong Yu, Jennifer L. Tenor, et al. “Regulatory Mechanism of the Atypical AP-1-Like Transcription Factor Yap1 in Cryptococcus neoformans.” Msphere 4, no. 6 (November 20, 2019). https://doi.org/10.1128/mSphere.00785-19.

PMID
31748248
Full Text

Pages