John Robert Perfect, MD

Professor of Medicine
James B. Duke Distinguished Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan, Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., The University of Toledo, 1974

Publications

Baddley, John W., Sharon C-A Chen, Carrie Huisingh, Kaitlin Benedict, Emilio E. DeBess, Eleni Galanis, Brendan R. Jackson, et al. “MSG07: An International Cohort Study Comparing Epidemiology and Outcomes of Patients With Cryptococcus neoformans or Cryptococcus gattii Infections.” Clin Infect Dis 73, no. 7 (October 5, 2021): 1133–41. https://doi.org/10.1093/cid/ciab268.

PMID
33772538
Full Text

Stott, Katharine Elizabeth, Angela Loyse, Joe N. Jarvis, Melanie Alufandika, Thomas Stephen Harrison, Henry C. Mwandumba, Jeremy N. Day, et al. “Cryptococcal meningoencephalitis: time for action.” Lancet Infect Dis 21, no. 9 (September 2021): e259–71. https://doi.org/10.1016/S1473-3099(20)30771-4.

PMID
33872594
Full Text

Toplis, Barbra, Caylin Bosch, Marietjie Stander, Malcolm Taylor, John R. Perfect, and Alfred Botha. “A link between urease and polyamine metabolism in Cryptococcus neoformans.” Microb Pathog 158 (September 2021): 105076. https://doi.org/10.1016/j.micpath.2021.105076.

PMID
34216740
Full Text

Rudramurthy, Shivaprakash M., Martin Hoenigl, Jacques F. Meis, Oliver A. Cornely, Valliappan Muthu, Jean Pierre Gangneux, John Perfect, Arunaloke Chakrabarti, and Arunaloke ECMM and ISHAM. “ECMM/ISHAM recommendations for clinical management of COVID-19 associated mucormycosis in low- and middle-income countries.” Mycoses 64, no. 9 (September 2021): 1028–37. https://doi.org/10.1111/myc.13335.

PMID
34133816
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Thu, Nguyen T. M., Jasper F. W. Chan, Vo Trieu Ly, Hoa T. Ngo, Ha T. A. Hien, Nguyen P. H. Lan, Nguyen V. V. Chau, et al. “Superiority of a Novel Mp1p Antigen Detection Enzyme Immunoassay Compared to Standard BACTEC Blood Culture in the Diagnosis of Talaromycosis.” Clin Infect Dis 73, no. 2 (July 15, 2021): e330–36. https://doi.org/10.1093/cid/ciaa826.

PMID
32564074
Full Text

Koehler, Philipp, Matteo Bassetti, Arunaloke Chakrabarti, Sharon C. A. Chen, Arnaldo Lopes Colombo, Martin Hoenigl, Nikolay Klimko, et al. “Defining and managing COVID-19-associated pulmonary aspergillosis: the 2020 ECMM/ISHAM consensus criteria for research and clinical guidance.” Lancet Infect Dis 21, no. 6 (June 2021): e149–62. https://doi.org/10.1016/S1473-3099(20)30847-1.

PMID
33333012
Full Text

Williams, Wilton B., R Ryan Meyerhoff, R. J. Edwards, Hui Li, Kartik Manne, Nathan I. Nicely, Rory Henderson, et al. “Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.” Cell 184, no. 11 (May 27, 2021): 2955-2972.e25. https://doi.org/10.1016/j.cell.2021.04.042.

PMID
34019795
Full Text

Fu, Man Shun, Livia C. Liporagi-Lopes, Samuel R. Dos Santos, Jennifer L. Tenor, John R. Perfect, Christina A. Cuomo, and Arturo Casadevall. “Amoeba Predation of Cryptococcus neoformans Results in Pleiotropic Changes to Traits Associated with Virulence.” Mbio 12, no. 2 (April 27, 2021). https://doi.org/10.1128/mbio.00567-21.

PMID
33906924
Full Text

Temfack, Elvis, Jean Joel Bigna Rim, Rene Spijker, Angela Loyse, Tom Chiller, Peter G. Pappas, John Perfect, et al. “Cryptococcal Antigen in Serum and Cerebrospinal Fluid for Detecting Cryptococcal Meningitis in Adults Living With Human Immunodeficiency Virus: Systematic Review and Meta-Analysis of Diagnostic Test Accuracy Studies.” Clin Infect Dis 72, no. 7 (April 8, 2021): 1268–78. https://doi.org/10.1093/cid/ciaa1243.

PMID
32829406
Full Text

Montoya, Marhiah C., Paul M. Magwene, and John R. Perfect. “Associations between Cryptococcus Genotypes, Phenotypes, and Clinical Parameters of Human Disease: A Review.” J Fungi (Basel) 7, no. 4 (March 30, 2021). https://doi.org/10.3390/jof7040260.

PMID
33808500
Full Text

Pages