John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Paul DiMondi, V, Townsend, ML, Johnson, M, and Durkin, M. "Antifungal catheter lock therapy for the management of a persistent Candida albicans bloodstream infection in an adult receiving hemodialysis." Pharmacotherapy 34, no. 7 (July 2014): e120-e127.

PMID
24782326
Full Text

Kung, H-C, Johnson, MD, Drew, RH, Saha-Chaudhuri, P, and Perfect, JR. "Clinical effectiveness of posaconazole versus fluconazole as antifungal prophylaxis in hematology-oncology patients: a retrospective cohort study." Cancer Medicine 3, no. 3 (June 2014): 667-673.

PMID
24644249
Full Text

Tseng, HK, Cho, WL, Liu, CP, Jong, JY, and Perfect, JR. "Verification of Cryptococcus neoformans Gene deletion mutants related to traverse the blood-brain barrier." May 2014.

Scholars@Duke

Perfect, JR. "2010 IDSA cryptococcal guidelines: a dynamic document; does it need updating?." May 2014.

Scholars@Duke

Smeekens, SP, Malireddi, RK, Plantinga, TS, Buffen, K, Oosting, M, Joosten, LAB, Kullberg, BJ, Perfect, JR, Scott, WK, van de Veerdonk, FL, Xavier, RJ, van de Vosse, E, Kanneganti, T-D, Johnson, MD, and Netea, MG. "Autophagy is redundant for the host defense against systemic Candida albicans infections." Eur J Clin Microbiol Infect Dis 33, no. 5 (May 2014): 711-722.

PMID
24202731
Full Text

Rosentul, DC, Plantinga, TS, Farcas, M, Oosting, M, Hamza, OJM, Scott, WK, Alexander, BD, Yang, JC, Laird, GM, Joosten, LAB, van der Meer, JWM, Perfect, JR, Kullberg, B-J, van der Ven, AJAM, Johnson, MD, and Netea, MG. "Role of autophagy genetic variants for the risk of Candida infections." Medical Mycology 52, no. 4 (May 2014): 333-341.

PMID
24713404
Full Text

Perfect, JR. "Cryptococcosis: a model for the understanding of infectious diseases." The Journal of Clinical Investigation 124, no. 5 (May 2014): 1893-1895.

PMID
24743152
Full Text

Horan, JL, and Perfect, JR. "Fungal meningitis." In Infections of the Central Nervous System: Fourth Edition. April 21, 2014.

Scholars@Duke

Janbon, G, Ormerod, KL, Paulet, D, Byrnes, EJ, Yadav, V, Chatterjee, G, Mullapudi, N, Hon, C-C, Billmyre, RB, Brunel, F, Bahn, Y-S, Chen, W, Chen, Y, Chow, EWL, Coppée, J-Y, Floyd-Averette, A, Gaillardin, C, Gerik, KJ, Goldberg, J, Gonzalez-Hilarion, S, Gujja, S, Hamlin, JL, Hsueh, Y-P, Ianiri, G, Jones, S, Kodira, CD, Kozubowski, L, Lam, W, Marra, M, Mesner, LD, Mieczkowski, PA, Moyrand, F, Nielsen, K, Proux, C, Rossignol, T, Schein, JE, Sun, S, Wollschlaeger, C, Wood, IA, Zeng, Q, Neuvéglise, C, Newlon, CS, Perfect, JR, Lodge, JK, Idnurm, A, Stajich, JE, Kronstad, JW, Sanyal, K, Heitman, J, Fraser, JA, Cuomo, CA, and Dietrich, FS. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation." Plos Genetics 10, no. 4 (April 17, 2014): e1004261-null.

PMID
24743168
Full Text

Al Thaqafi, AHO, Farahat, FM, Al Harbi, MI, Al Amri, AFW, and Perfect, JR. "Predictors and outcomes of Candida bloodstream infection: eight-year surveillance, western Saudi Arabia." International Journal of Infectious Diseases : Ijid : Official Publication of the International Society for Infectious Diseases 21 (April 2014): 5-9.

PMID
24468816
Full Text

Pages