John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Janout, Vaclav, Celine Bienvenu, Wiley Schell, John R. Perfect, and Steven L. Regen. “Molecular umbrella-amphotericin B conjugates..” Bioconjug Chem 25, no. 8 (August 20, 2014): 1408–11. https://doi.org/10.1021/bc500277v.

PMID
25068916
Full Text

Jarvis, Joseph N., Tihana Bicanic, Angela Loyse, Graeme Meintjes, Louise Hogan, Chrissy H. Roberts, Shmuel Shoham, John R. Perfect, Nelesh P. Govender, and Thomas S. Harrison. “Very low levels of 25-hydroxyvitamin D are not associated with immunologic changes or clinical outcome in South African patients with HIV-associated cryptococcal meningitis..” Clin Infect Dis 59, no. 4 (August 15, 2014): 493–500. https://doi.org/10.1093/cid/ciu349.

PMID
24825871
Full Text

Paul DiMondi, V., Mary L. Townsend, Melissa Johnson, and Michael Durkin. “Antifungal catheter lock therapy for the management of a persistent Candida albicans bloodstream infection in an adult receiving hemodialysis..” Pharmacotherapy 34, no. 7 (July 2014): e120–27. https://doi.org/10.1002/phar.1433.

PMID
24782326
Full Text

Kung, Hsiang-Chi, Melissa D. Johnson, Richard H. Drew, Paramita Saha-Chaudhuri, and John R. Perfect. “Clinical effectiveness of posaconazole versus fluconazole as antifungal prophylaxis in hematology-oncology patients: a retrospective cohort study..” Cancer Med 3, no. 3 (June 2014): 667–73. https://doi.org/10.1002/cam4.225.

PMID
24644249
Full Text

Tseng, H. K., W. L. Cho, C. P. Liu, J. Y. Jong, and J. R. Perfect. “Verification of Cryptococcus neoformans Gene deletion mutants related to traverse the blood-brain barrier.” In Mycoses, 57:55–56. WILEY-BLACKWELL, 2014.

Scholars@Duke

Perfect, J. R. “2010 IDSA cryptococcal guidelines: a dynamic document; does it need updating?.” In Mycoses, 57:28–28. WILEY-BLACKWELL, 2014.

Scholars@Duke

Rosentul, Diana C., Theo S. Plantinga, Marius Farcas, Marije Oosting, Omar J. M. Hamza, William K. Scott, Barbara D. Alexander, et al. “Role of autophagy genetic variants for the risk of Candida infections..” Med Mycol 52, no. 4 (May 2014): 333–41. https://doi.org/10.1093/mmy/myt035.

PMID
24713404
Full Text

Smeekens, S. P., R. K. Malireddi, T. S. Plantinga, K. Buffen, M. Oosting, L. A. B. Joosten, B. J. Kullberg, et al. “Autophagy is redundant for the host defense against systemic Candida albicans infections..” Eur J Clin Microbiol Infect Dis 33, no. 5 (May 2014): 711–22. https://doi.org/10.1007/s10096-013-2002-x.

PMID
24202731
Full Text

Horan, J. L., and J. R. Perfect. “Fungal meningitis.” In Infections of the Central Nervous System: Fourth Edition, 2014.

Scholars@Duke

Pages