John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Kwon-Chung, Kyung J., John E. Bennett, Brian L. Wickes, Wieland Meyer, Christina A. Cuomo, Kurt R. Wollenburg, Tihana A. Bicanic, et al. “The Case for Adopting the "Species Complex" Nomenclature for the Etiologic Agents of Cryptococcosis.” Msphere 2, no. 2 (February 2017). https://doi.org/10.1128/mSphere.00357-16.

Full Text

Messina, Julia A., Eileen K. Maziarz, Andrej Spec, Dimitrios P. Kontoyiannis, and John R. Perfect. “Disseminated Cryptococcosis With Brain Involvement in Patients With Chronic Lymphoid Malignancies on Ibrutinib..” Open Forum Infect Dis 4, no. 1 (2017). https://doi.org/10.1093/ofid/ofw261.

PMID
28480254
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Matzaraki, Vasiliki, Mark S. Gresnigt, Martin Jaeger, Isis Ricaño-Ponce, Melissa D. Johnson, Marije Oosting, Lude Franke, et al. “An integrative genomics approach identifies novel pathways that influence candidaemia susceptibility..” Plos One 12, no. 7 (2017). https://doi.org/10.1371/journal.pone.0180824.

PMID
28727728
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Messina, J. A., E. K. Maziarz, A. Spec, D. P. Kontoyiannis, and J. R. Perfect. “Disseminated cryptococcosis with brain involvement in patients with chronic lymphoid malignancies on ibrutinib.” Open Forum Infectious Diseases 4, no. 1 (January 1, 2017). https://doi.org/10.1093/oid/ofw261.

Full Text

Kwon-Chung, Kyung J., John E. Bennett, Brian L. Wickes, Wieland Meyer, Christina A. Cuomo, Kurt R. Wollenburg, Tihana A. Bicanic, et al. “The Case for Adopting the "Species Complex" Nomenclature for the Etiologic Agents of Cryptococcosis..” Msphere 2, no. 1 (January 2017). https://doi.org/10.1128/mSphere.00357-16.

PMID
28101535
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Chen, Yuan, and John R. Perfect. “Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ..” Methods Mol Biol 1492 (2017): 197–202. https://doi.org/10.1007/978-1-4939-6442-0_14.

PMID
27822866
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Yu, Yuming, Mary J. Sabulski, Wiley A. Schell, Marcos M. Pires, John R. Perfect, and Steven L. Regen. “Simple Strategy for Taming Membrane-Disrupting Antibiotics..” Bioconjug Chem 27, no. 12 (December 21, 2016): 2850–53. https://doi.org/10.1021/acs.bioconjchem.6b00629.

PMID
27801580
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Roth, Susanne, Hanna Bergmann, Martin Jaeger, Assa Yeroslaviz, Konstantin Neumann, Paul-Albert Koenig, Clarissa Prazeres da Costa, et al. “Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity..” Cell Rep 17, no. 10 (December 6, 2016): 2572–83. https://doi.org/10.1016/j.celrep.2016.11.018.

PMID
27926862
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Shorman, M., D. Evans, C. Gibson, and J. Perfect. “Cases of disseminated cryptococcosis in intravenous drug abusers without HIV infection: A new risk factor?.” Medical Mycology Case Reports 14 (December 1, 2016): 17–19. https://doi.org/10.1016/j.mmcr.2016.12.003.

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