John Robert Perfect, MD

Professor of Medicine
James B. Duke Distinguished Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan, Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., The University of Toledo, 1974

Publications

Messina, Julia A., Cameron R. Wolfe, Marion Hemmersbach-Miller, Carmelo Milano, Jamie L. Todd, John Reynolds, Barbara D. Alexander, Wiley A. Schell, Christina A. Cuomo, and John R. Perfect. “Genomic characterization of recurrent mold infections in thoracic transplant recipients.” Transpl Infect Dis 20, no. 5 (October 2018): e12935. https://doi.org/10.1111/tid.12935.

PMID
29851203
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Smilnak, Gordon J., Lefko T. Charalambous, Drew Cutshaw, Alykhan M. Premji, Charles D. Giamberardino, Christi G. Ballard, Andrew P. Bartuska, et al. “Novel Treatment of Cryptococcal Meningitis via Neurapheresis Therapy.” J Infect Dis 218, no. 7 (August 24, 2018): 1147–54. https://doi.org/10.1093/infdis/jiy286.

PMID
29788431
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Shaw, Karen Joy, Wiley A. Schell, Jonathan Covel, Gisele Duboc, C. Giamberardino, Mili Kapoor, Molly Moloney, et al. “In Vitro and In Vivo Evaluation of APX001A/APX001 and Other Gwt1 Inhibitors against Cryptococcus.” Antimicrobial Agents and Chemotherapy 62, no. 8 (August 1, 2018). https://doi.org/10.1128/ACC00523;e00523-18.

Full Text

Cornely, Oliver A., Kathleen M. Mullane, Luis Ostrosky-Zeichner, Rochelle M. Maher, Rodney Croos-Dabrera, Qiaoyang Lu, Christopher Lademacher, et al. “Isavuconazole for treatment of rare invasive fungal diseases.” Mycoses 61, no. 8 (August 2018): 518–33. https://doi.org/10.1111/myc.12778.

PMID
29611246
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Shaw, Karen Joy, Wiley A. Schell, Jonathan Covel, Gisele Duboc, C. Giamberardino, Mili Kapoor, Molly Moloney, et al. “In Vitro and In Vivo Evaluation of APX001A/APX001 and Other Gwt1 Inhibitors against Cryptococcus.” Antimicrob Agents Chemother 62, no. 8 (August 2018). https://doi.org/10.1128/AAC.00523-18.

PMID
29891599
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Hussaini, Syed Mohammed Qasim, Deng Madut, Betty C. Tong, Elizabeth N. Pavlisko, Wiley A. Schell, John R. Perfect, and Nathan M. Thielman. “Pulmonary blastomycosis presenting as primary lung cancer.” Bmc Infect Dis 18, no. 1 (July 18, 2018): 336. https://doi.org/10.1186/s12879-018-3244-0.

PMID
30021526
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Maskarinec, Stacey A., Zehra Parlak, Qing Tu, Vrad Levering, Stefan Zauscher, Gabriel P. López, Vance G. Fowler, and John R. Perfect. “On-demand release of Candida albicans biofilms from urinary catheters by mechanical surface deformation.” Biofouling 34, no. 6 (July 2018): 595–604. https://doi.org/10.1080/08927014.2018.1474461.

PMID
29897277
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Mourad, A., melissa Johnson, and john Perfect. “Predictors of Mortality in Patients Receiving Voriconazole.” Atlanta, GA: American Society of Microbiology - Microbe, 2018.

Scholars@Duke

Garcia-Santamarina, Sarela, Richard A. Festa, Aaron D. Smith, Chen-Hsin Yu, Corinna Probst, Chen Ding, Christina M. Homer, et al. “Genome-wide analysis of the regulation of Cu metabolism in Cryptococcus neoformans.” Mol Microbiol 108, no. 5 (June 2018): 473–94. https://doi.org/10.1111/mmi.13960.

PMID
29608794
Full Text

Hommel, Benjamin, Liliane Mukaremera, Radames J. B. Cordero, Carolina Coelho, Christopher A. Desjardins, Aude Sturny-Leclère, Guilhem Janbon, et al. “Titan cells formation in Cryptococcus neoformans is finely tuned by environmental conditions and modulated by positive and negative genetic regulators.” Plos Pathog 14, no. 5 (May 2018): e1006982. https://doi.org/10.1371/journal.ppat.1006982.

PMID
29775480
Full Text

Pages