John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Lestner, Jodi, Laura McEntee, Adam Johnson, Joanne Livermore, Sarah Whalley, Julie Schwartz, John R. Perfect, Thomas Harrison, and William Hope. “Experimental Models of Short Courses of Liposomal Amphotericin B for Induction Therapy for Cryptococcal Meningitis..” Antimicrob Agents Chemother 61, no. 6 (June 2017). https://doi.org/10.1128/AAC.00090-17.

PMID
28320715
Full Text

Chen, Yuan, Rhys A. Farrer, Charles Giamberardino, Sharadha Sakthikumar, Alexander Jones, Timothy Yang, Jennifer L. Tenor, et al. “Microevolution of Serial Clinical Isolates of Cryptococcus neoformans var. grubii and C. gattii..” Mbio 8, no. 2 (March 7, 2017). https://doi.org/10.1128/mBio.00166-17.

PMID
28270580
Full Text

Farber, S Harrison, Kelly R. Murphy, Carter M. Suryadevara, Ranjith Babu, Siyun Yang, Liqi Feng, Jichun Xie, John R. Perfect, and Shivanand P. Lad. “Comparing outcomes of early, late, and non-surgical management of intraspinal abscess..” J Clin Neurosci 36 (February 2017): 64–71. https://doi.org/10.1016/j.jocn.2016.10.035.

PMID
27836393
Full Text

Parente, V., R. H. Clark, L. Ku, C. Fennell, M. Johnson, E. Morris, A. Romaine, et al. “Risk factors for group B streptococcal disease in neonates of mothers with negative antenatal testing..” J Perinatol 37, no. 2 (February 2017): 157–61. https://doi.org/10.1038/jp.2016.201.

PMID
27853322
Full Text

Kwon-Chung, Kyung J., John E. Bennett, Brian L. Wickes, Wieland Meyer, Christina A. Cuomo, Kurt R. Wollenburg, Tihana A. Bicanic, et al. “The Case for Adopting the "Species Complex" Nomenclature for the Etiologic Agents of Cryptococcosis.” Msphere 2, no. 2 (February 2017). https://doi.org/10.1128/mSphere.00357-16.

Full Text

Kwon-Chung, Kyung J., John E. Bennett, Brian L. Wickes, Wieland Meyer, Christina A. Cuomo, Kurt R. Wollenburg, Tihana A. Bicanic, et al. “The Case for Adopting the "Species Complex" Nomenclature for the Etiologic Agents of Cryptococcosis..” Msphere 2, no. 1 (January 2017). https://doi.org/10.1128/mSphere.00357-16.

PMID
28101535
Full Text

Chen, Yuan, and John R. Perfect. “Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ..” Methods Mol Biol 1492 (2017): 197–202. https://doi.org/10.1007/978-1-4939-6442-0_14.

PMID
27822866
Full Text

Pages