John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Del Poeta, M, Schell, WA, Dykstra, CC, Jones, SK, Tidwell, RR, Kumar, A, Boykin, DW, and Perfect, JR. "In vitro antifungal activities of a series of dication-substituted carbazoles, furans, and benzimidazoles." Antimicrob Agents Chemother 42, no. 10 (October 1998): 2503-2510.

PMID
9756748
Scholars@Duke

Johnson, MD, Drew, RH, and Perfect, JR. "Chest discomfort associated with liposomal amphotericin B: report of three cases and review of the literature." Pharmacotherapy 18, no. 5 (September 1998): 1053-1061. (Review)

PMID
9758315
Scholars@Duke

Schell, WA, Liu, K, Howell, DN, and Perfect, JR. "The phenomenon of adventitious sporulation." AMERICAN JOURNAL OF CLINICAL PATHOLOGY 110, no. 1 (July 1998): 129-129.

Scholars@Duke

Walsh, TJ, Hiemenz, JW, Seibel, NL, Perfect, JR, Horwith, G, Lee, L, Silber, JL, DiNubile, MJ, Reboli, A, Bow, E, Lister, J, and Anaissie, EJ. "Amphotericin B lipid complex for invasive fungal infections: analysis of safety and efficacy in 556 cases." Clin Infect Dis 26, no. 6 (June 1998): 1383-1396.

PMID
9636868
Scholars@Duke

Firestine, SM, Misialek, S, Toffaletti, DL, Klem, TJ, Perfect, JR, and Davisson, VJ. "Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis." Arch Biochem Biophys 351, no. 1 (March 1, 1998): 123-134.

PMID
9500840
Full Text

Perfect, JR, Wong, B, Chang, YC, Kwon-Chung, KJ, and Williamson, PR. "Cryptococcus neoformans: virulence and host defences." Med Mycol 36 Suppl 1 (1998): 79-86. (Review)

PMID
9988495
Scholars@Duke

Perfect, JR, Wong, B, Chang, YC, Kwon-Chung, KJ, and Williamson, PR. "Cryptococcus neoformans: Virulence and host defences." Medical Mycology, Supplement 36, no. 1 (1998): 79-86.

Scholars@Duke

Liu, K, Howell, DN, Perfect, JR, and Schell, WA. "Morphologic criteria for the preliminary identification of Fusarium, Paecilomyces, and Acremonium species by histopathology." Am J Clin Pathol 109, no. 1 (January 1998): 45-54.

PMID
9426517
Scholars@Duke

Alspaugh, JA, Perfect, JR, and Heitman, J. "Cryptococcus neoformans mating and virulence are regulated by the G-protein alpha subunit GPA1 and cAMP." Genes Dev 11, no. 23 (December 1, 1997): 3206-3217.

PMID
9389652
Scholars@Duke

Alexander, BD, and Perfect, JR. "Antifungal resistance trends towards the year 2000. Implications for therapy and new approaches." Drugs 54, no. 5 (November 1997): 657-678. (Review)

PMID
9360056
Scholars@Duke

Pages