John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Hart, BE, Asrican, R, Lim, S-Y, Sixsmith, JD, Lukose, R, Souther, SJR, Rayasam, SDG, Saelens, JW, Chen, C-J, Seay, SA, Berney-Meyer, L, Magtanong, L, Vermeul, K, Pajanirassa, P, Jimenez, AE, Ng, TW, Tobin, DM, Porcelli, SA, Larsen, MH, Schmitz, JE, Haynes, BF, Jacobs, WR, Lee, S, and Frothingham, R. "Stable Expression of Lentiviral Antigens by Quality-Controlled Recombinant Mycobacterium bovis BCG Vectors." Clinical and vaccine immunology : CVI 22, no. 7 (July 2015): 726-741.

PMID
25924766
Full Text

Molefi, M, Chofle, AA, Molloy, SF, Kalluvya, S, Changalucha, JM, Cainelli, F, Leeme, T, Lekwape, N, Goldberg, DW, Haverkamp, M, Bisson, GP, Perfect, JR, Letang, E, Fenner, L, Meintjes, G, Burton, R, Makadzange, T, Ndhlovu, CE, Hope, W, Harrison, TS, and Jarvis, JN. "AMBITION-cm: intermittent high dose AmBisome on a high dose fluconazole backbone for cryptococcal meningitis induction therapy in sub-Saharan Africa: study protocol for a randomized controlled trial." Trials 16 (June 17, 2015): 276-.

PMID
26081985
Full Text

Allen, D, Wilson, D, Drew, R, and Perfect, J. "Azole antifungals: 35 years of invasive fungal infection management." Expert review of anti-infective therapy 13, no. 6 (June 2015): 787-798. (Review)

PMID
25843556
Full Text

Tobin, DM. "Host-Directed Therapies for Tuberculosis." Cold Spring Harbor perspectives in medicine 5, no. 10 (May 18, 2015). (Review)

PMID
25986592
Full Text

Perfect, JR, and Bicanic, T. "Cryptococcosis diagnosis and treatment: What do we know now." Fungal genetics and biology : FG & B 78 (May 2015): 49-54. (Review)

PMID
25312862
Full Text

Jaeger, M, van der Lee, R, Cheng, S-C, Johnson, MD, Kumar, V, Ng, A, Plantinga, TS, Smeekens, SP, Oosting, M, Wang, X, Barchet, W, Fitzgerald, K, Joosten, LAB, Perfect, JR, Wijmenga, C, van de Veerdonk, FL, Huynen, MA, Xavier, RJ, Kullberg, B-J, and Netea, MG. "The RIG-I-like helicase receptor MDA5 (IFIH1) is involved in the host defense against Candida infections." European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 34, no. 5 (May 2015): 963-974.

PMID
25579795
Full Text

American College of Clinical Pharmacy, , McBane, SE, Dopp, AL, Abe, A, Benavides, S, Chester, EA, Dixon, DL, Dunn, M, Johnson, MD, Nigro, SJ, Rothrock-Christian, T, Schwartz, AH, Thrasher, K, and Walker, S. "Collaborative drug therapy management and comprehensive medication management-2015." Pharmacotherapy 35, no. 4 (April 2015): e39-e50.

PMID
25884536
Full Text

Mandegari, E, Fu, L, Arambú, C, Montoya, S, Peña, A, Johnson, KM, Perfect, JR, and Caniza, MA. "Mucormycosis Rhinosinusitis at Diagnosis of Acute Lymphoblastic Leukemia: Diagnostics and Management Challenges in a Low-Middle-income Country." Journal of pediatric hematology/oncology 37, no. 3 (April 2015): e173-e177.

PMID
24942033
Full Text

Arnold, CJ, Johnson, M, Bayer, AS, Bradley, S, Giannitsioti, E, Miró, JM, Tornos, P, Tattevin, P, Strahilevitz, J, Spelman, D, Athan, E, Nacinovich, F, Fortes, CQ, Lamas, C, Barsic, B, Fernández-Hidalgo, N, Muñoz, P, and Chu, VH. "Candida infective endocarditis: an observational cohort study with a focus on therapy." Antimicrobial agents and chemotherapy 59, no. 4 (April 2015): 2365-2373.

PMID
25645855
Full Text

de Hoog, GS, Chaturvedi, V, Denning, DW, Dyer, PS, Frisvad, JC, Geiser, D, Gräser, Y, Guarro, J, Haase, G, Kwon-Chung, K-J, Meis, JF, Meyer, W, Pitt, JI, Samson, RA, Taylor, JW, Tintelnot, K, Vitale, RG, Walsh, TJ, Lackner, M, and ISHAM Working Group on Nomenclature of Medical Fungi, . "Name changes in medically important fungi and their implications for clinical practice." Journal of clinical microbiology 53, no. 4 (April 2015): 1056-1062.

PMID
25297326
Full Text

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