John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Marty, FM, Ostrosky-Zeichner, L, Cornely, OA, Mullane, KM, Perfect, JR, Thompson, GR, Alangaden, GJ, Brown, JM, Fredricks, DN, Heinz, WJ, Herbrecht, R, Klimko, N, Klyasova, G, Maertens, JA, Melinkeri, SR, Oren, I, Pappas, PG, Ráčil, Z, Rahav, G, Santos, R, Schwartz, S, Vehreschild, JJ, Young, J-AH, Chetchotisakd, P, Jaruratanasirikul, S, Kanj, SS, Engelhardt, M, Kaufhold, A, Ito, M, Lee, M, Sasse, C, Maher, RM, Zeiher, B, Vehreschild, MJGT, and VITAL and FungiScope Mucormycosis Investigators, . "Isavuconazole treatment for mucormycosis: a single-arm open-label trial and case-control analysis." The Lancet. Infectious Diseases 16, no. 7 (July 2016): 828-837.

PMID
26969258
Full Text

Miao, Y, Tenor, JL, Toffaletti, DL, Washington, EJ, Liu, J, Shadrick, WR, Schumacher, MA, Lee, RE, Perfect, JR, and Brennan, RG. "Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis." Proceedings of the National Academy of Sciences of the United States of America 113, no. 26 (June 15, 2016): 7148-7153.

PMID
27307435
Full Text

Perfect, JR. ""Is there an emerging need for new antifungals?"." Expert Opinion on Emerging Drugs 21, no. 2 (June 2016): 129-131.

PMID
26883732
Full Text

Perfect, JR. "Editorial Commentary: Life-saving Antimicrobial Drugs: What Are We Doing to Pricing and Availability?." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America 62, no. 12 (June 2016): 1569-1570.

PMID
27009252
Full Text

Maskarinec, SA, Johnson, MD, and Perfect, JR. "Genetic Susceptibility to Fungal Infections: What is in the Genes?." Current Clinical Microbiology Reports 3, no. 2 (June 2016): 81-91.

PMID
27547700
Full Text

Homer, CM, Summers, DK, Goranov, AI, Clarke, SC, Wiesner, DL, Diedrich, JK, Moresco, JJ, Toffaletti, D, Upadhya, R, Caradonna, I, Petnic, S, Pessino, V, Cuomo, CA, Lodge, JK, Perfect, J, Yates, JR, Nielsen, K, Craik, CS, and Madhani, HD. "Intracellular Action of a Secreted Peptide Required for Fungal Virulence." Cell host & microbe 19, no. 6 (June 2016): 849-864.

PMID
27212659
Full Text

Bahr, NC, Marais, S, Caws, M, van Crevel, R, Wilkinson, RJ, Tyagi, JS, Thwaites, GE, Boulware, DR, and Tuberculous Meningitis International Research Consortium, . "GeneXpert MTB/Rif to Diagnose Tuberculous Meningitis: Perhaps the First Test but not the Last." May 2016.

PMID
26966284
Full Text

Berg, RD, Levitte, S, O'Sullivan, MP, O'Leary, SM, Cambier, CJ, Cameron, J, Takaki, KK, Moens, CB, Tobin, DM, Keane, J, and Ramakrishnan, L. "Lysosomal Disorders Drive Susceptibility to Tuberculosis by Compromising Macrophage Migration." Cell 165, no. 1 (March 2016): 139-152.

PMID
27015311
Full Text

Maziarz, EK, and Perfect, JR. "Cryptococcosis." Infectious Disease Clinics of North America 30, no. 1 (March 2016): 179-206. (Review)

PMID
26897067
Full Text

Caza, M, Hu, G, Price, M, Perfect, JR, and Kronstad, JW. "The Zinc Finger Protein Mig1 Regulates Mitochondrial Function and Azole Drug Susceptibility in the Pathogenic Fungus Cryptococcus neoformans." Msphere 1, no. 1 (January 13, 2016).

PMID
27303693
Full Text

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