John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • M.D., University of Toledo, 1974

Publications

Inoue, M, Arikawa, T, Chen, Y-H, Moriwaki, Y, Price, M, Brown, M, Perfect, JR, and Shinohara, ML. "T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation." Proceedings of the National Academy of Sciences of the United States of America 111, no. 14 (April 2014): 5295-5300.

PMID
24706909
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Al Thaqafi, AHO, Farahat, FM, Al Harbi, MI, Al Amri, AFW, and Perfect, JR. "Predictors and outcomes of Candida bloodstream infection: eight-year surveillance, western Saudi Arabia." International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 21 (April 2014): 5-9.

PMID
24468816
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Spivey, JR, Drew, RH, and Perfect, JR. "Future strategies for the treatment of cryptococcal meningoencephalitis in pediatric patients." Expert Opinion on Orphan Drugs 2, no. 3 (March 2014): 245-257.

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Chen, Y, Toffaletti, DL, Tenor, JL, Litvintseva, AP, Fang, C, Mitchell, TG, McDonald, TR, Nielsen, K, Boulware, DR, Bicanic, T, and Perfect, JR. "The Cryptococcus neoformans transcriptome at the site of human meningitis." mBio 5, no. 1 (February 4, 2014): e01087-e01013.

PMID
24496797
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Chen, Y, Toffaletti, DL, Tenor, JL, Litvintseva, AP, Fang, C, Mitchell, TG, McDonald, TR, Nielsen, K, Boulware, DR, Bicanic, T, and Perfect, JR. "The Cryptococcus neoformans transcriptome at the site of human meningitis." mBio 5, no. 1 (February 4, 2014).

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Holmer, SM, Evans, KS, Asfaw, YG, Saini, D, Schell, WA, Ledford, JG, Frothingham, R, Wright, JR, Sempowski, GD, Perfect, JR, and Pirofski, L. "Impact of surfactant protein d, interleukin-5, and eosinophilia on cryptococcosis." Infection and immunity 82, no. 2 (February 2014): 683-693.

PMID
24277798
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Livermore, J, Howard, SJ, Sharp, AD, Goodwin, J, Gregson, L, Felton, T, Schwartz, JA, Walker, C, Moser, B, Müller, W, Harrison, TS, Perfect, JR, and Hope, WW. "Efficacy of an abbreviated induction regimen of amphotericin B deoxycholate for cryptococcal meningoencephalitis: 3 days of therapy is equivalent to 14 days." mBio 5, no. 1 (January 28, 2014): e00725-e00713.

PMID
24473125
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Livermore, J, Howard, SJ, Sharp, AD, Goodwin, J, Gregson, L, Felton, T, Schwartz, JA, Walker, C, Moser, B, Müller, W, Harrison, TS, Perfect, JR, and Hope, WW. "Efficacy of an abbreviated induction regimen of amphotericin B deoxycholate for cryptococcal meningoencephalitis: 3 days of therapy is equivalent to 14 days." mBio 5, no. 1 (January 28, 2014).

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Smeekens, SP, Malireddi, RK, Plantinga, TS, Buffen, K, Oosting, M, Joosten, LAB, Kullberg, BJ, Perfect, JR, Scott, WK, Van De Veerdonk, FL, Xavier, RJ, Van De Vosse, E, Kanneganti, TD, Johnson, MD, and Netea, MG. "Autophagy is redundant for the host defense against systemic Candida albicans infections." European Journal of Clinical Microbiology and Infectious Diseases 33, no. 5 (January 1, 2014): 711-722.

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Perfect, JR. "Fungal Infections of the Central Nervous System." (January 1, 2014): 925-945. (Chapter)

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