John Robert Perfect, MD

Professor of Medicine
James B. Duke Professor of Medicine
Chief, Division of Infectious Diseases
Professor in Molecular Genetics and Microbiology
Campus mail Duke Box 102359, Durham, NC 27710
Phone (919) 684-4016
Email address perfe001@mc.duke.edu

Research in my laboratory focuses around several aspects of medical mycology. We are investigating antifungal agents (new and old) in animal models of candida and cryptococcal infections. We have examined clinical correlation of in vitro antifungal susceptibility testing and with in vivo outcome. Our basic science project examines the molecular pathogenesis of cryptococcal infections. We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes. The goal of this work is to use C. neoformans as a model yeast system to identify molecular targets for antifungal drug development. There are a series of clinical trials in fungal infections which are being coordinated through this laboratory and my work also includes a series of antibiotic trials in various aspects of infections. Finally, we have now been awarded a NIH sponsored Mycology Unit for 5 years with 6 senior investigators which is focused on C. neoformans as a pathogenic model system, but will include multiple areas of medical mycology from diagnosis to treatment.

Education and Training

  • Fellow, Infectious Diseases, Duke University School of Medicine, 1977 - 1980
  • Resident, University of Michigan at Ann Arbor, 1975 - 1977
  • Intern, Riverside Methodist Hospital, 1974 - 1975
  • M.D., University of Toledo, 1974

Publications

Homer, CM, Summers, DK, Goranov, AI, Clarke, SC, Wiesner, DL, Diedrich, JK, Moresco, JJ, Toffaletti, D, Upadhya, R, Caradonna, I, Petnic, S, Pessino, V, Cuomo, CA, Lodge, JK, Perfect, J, Yates, JR, Nielsen, K, Craik, CS, and Madhani, HD. "Intracellular Action of a Secreted Peptide Required for Fungal Virulence." Cell host & microbe 19, no. 6 (June 2016): 849-864.

PMID
27212659
Full Text

Bahr, NC, Marais, S, Caws, M, van Crevel, R, Wilkinson, RJ, Tyagi, JS, Thwaites, GE, Boulware, DR, and Tuberculous Meningitis International Research Consortium, . "GeneXpert MTB/Rif to Diagnose Tuberculous Meningitis: Perhaps the First Test but not the Last." May 2016.

PMID
26966284
Full Text

Maziarz, EK, and Perfect, JR. "Cryptococcosis." Infectious Disease Clinics of North America 30, no. 1 (March 2016): 179-206. (Review)

PMID
26897067
Full Text

Berg, RD, Levitte, S, O'Sullivan, MP, O'Leary, SM, Cambier, CJ, Cameron, J, Takaki, KK, Moens, CB, Tobin, DM, Keane, J, and Ramakrishnan, L. "Lysosomal Disorders Drive Susceptibility to Tuberculosis by Compromising Macrophage Migration." Cell 165, no. 1 (March 2016): 139-152.

PMID
27015311
Full Text

Caza, M, Hu, G, Price, M, Perfect, JR, and Kronstad, JW. "The Zinc Finger Protein Mig1 Regulates Mitochondrial Function and Azole Drug Susceptibility in the Pathogenic Fungus Cryptococcus neoformans." mSphere 1, no. 1 (January 13, 2016).

PMID
27303693
Full Text

Swamydas, M, Gao, J-L, Break, TJ, Johnson, MD, Jaeger, M, Rodriguez, CA, Lim, JK, Green, NM, Collar, AL, Fischer, BG, Lee, C-CR, Perfect, JR, Alexander, BD, Kullberg, B-J, Netea, MG, Murphy, PM, and Lionakis, MS. "CXCR1-mediated neutrophil degranulation and fungal killing promote Candida clearance and host survival." Science Translational Medicine 8, no. 322 (January 2016): 322ra10-null.

PMID
26791948
Full Text

Matty, MA, Oehlers, SH, and Tobin, DM. "Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae." Methods in molecular biology (Clifton, N.J.) 1451 (January 2016): 207-223.

PMID
27464810
Full Text

Bazemore, TC, Maskarinec, SA, Zietlow, K, Hendershot, EF, and Perfect, JR. "Familial Adenomatous Polyposis Manifesting as Lactococcus Endocarditis: A Case Report and Review of the Association of Lactococcus with Underlying Gastrointestinal Disease." Case reports in infectious diseases 2016 (January 2016): 5805326-.

PMID
27818810
Full Text

Beerman, RW, Matty, MA, Au, GG, Looger, LL, Choudhury, KR, Keller, PJ, and Tobin, DM. "Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux." Cell Rep 13, no. 10 (December 15, 2015): 2107-2117.

PMID
26673320
Full Text

Saelens, JW, Lau-Bonilla, D, Moller, A, Medina, N, Guzmán, B, Calderón, M, Herrera, R, Sisk, DM, Xet-Mull, AM, Stout, JE, Arathoon, E, Samayoa, B, and Tobin, DM. "Whole genome sequencing identifies circulating Beijing-lineage Mycobacterium tuberculosis strains in Guatemala and an associated urban outbreak." Tuberculosis (Edinburgh, Scotland) 95, no. 6 (December 2015): 810-816.

PMID
26542222
Full Text

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